DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lin-2009-14 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lin G., Li D., de Carvalho LP., Deng H., Tao H., Vogt G., Wu K., Schneider J., Chidawanyika T., Warren JD., Li H., Nathan C. DIP original experiment: DIP-219558X Nature 2009 IMEx Inhibitors selective for mycobacterial versus human proteasomes. Lin G., Li D., de Carvalho LP., Deng H., Tao H., Vogt G., Wu K., Schneider J., Chidawanyika T., Warren JD., Li H., Nathan C. Only protein-protein interactions imex curation lin-2009-15 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lin G., Li D., de Carvalho LP., Deng H., Tao H., Vogt G., Wu K., Schneider J., Chidawanyika T., Warren JD., Li H., Nathan C. DIP original experiment: DIP-219559X Nature 2009 IMEx Inhibitors selective for mycobacterial versus human proteasomes. Lin G., Li D., de Carvalho LP., Deng H., Tao H., Vogt G., Wu K., Schneider J., Chidawanyika T., Warren JD., Li H., Nathan C. Only protein-protein interactions imex curation lin-2009-16 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Lin G., Li D., de Carvalho LP., Deng H., Tao H., Vogt G., Wu K., Schneider J., Chidawanyika T., Warren JD., Li H., Nathan C. DIP original experiment: DIP-219560X Nature 2009 IMEx Inhibitors selective for mycobacterial versus human proteasomes. Lin G., Li D., de Carvalho LP., Deng H., Tao H., Vogt G., Wu K., Schneider J., Chidawanyika T., Warren JD., Li H., Nathan C. Only protein-protein interactions imex curation psb_myctu Proteasome subunit beta Proteasome core protein PrcB Rv2110c prcB 20S proteasome beta subunit protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTWPLPDRLSINSLSGTPAVDLSSFTDFLRRQAPELLPASISGGAPLAGGDAQLPHGTTIVALKYPGGVVMAGDRRSTQGNMISGRDVRKVYITDDYTATGIAGTAAVAVEFARLYAVELEHYEKLEGVPLTFAGKINRLAIMVRGNLAAAMQGLLALPLLAGYDIHASDPQSAGRIVSFDAAGGWNIEEEGYQAVGSGSLFAKSSMKKLYSQVTDGDSGLRVAVEALYDAADDDSATGGPDLVRGIFPTAVIIDADGAVDVPESRIAELARAIIESRSGADTFGSDGGEK 3FC3FCD81231E129 mint psa_myctu Proteasome subunit alpha Proteasome core protein PrcA Rv2109c prcA 20S proteasome alpha subunit protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MSFPYFISPEQAMRERSELARKGIARAKSVVALAYAGGVLFVAENPSRSLQKISELYDRVGFAAAGKFNEFDNLRRGGIQFADTRGYAYDRRDVTGRQLANVYAQTLGTIFTEQAKPYEVELCVAEVAHYGETKRPELYRITYDGSIADEPHFVVMGGTTEPIANALKESYAENASLTDALRIAVAALRAGSADTSGGDQPTLGVASLEVAVLDANRPRRAFRRITGSALQALLVDQESPQSDGESSG 0356FED74869998A mint 20salpha-20sbeta-1 3196147 N/A 3196150 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3196151 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false 20salpha-20sbeta-2 3196148 N/A 3196150 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3196151 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false 20sbeta-20salpha-1 3196149 N/A 3196150 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3196151 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false