IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mairhofer-2009-1 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. rainer.prohaska@meduniwien.ac.at The Journal of Biological Chemistry 2009 imex curation Only protein-protein interactions imex curation mairhofer-2009-2 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. rainer.prohaska@meduniwien.ac.at The Journal of Biological Chemistry 2009 imex curation Only protein-protein interactions imex curation mairhofer-2009-3 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. Isolation of DRMs (Detergent Resistant Rafts). rainer.prohaska@meduniwien.ac.at The Journal of Biological Chemistry 2009 imex curation Only protein-protein interactions imex curation mairhofer-2009-4 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. rainer.prohaska@meduniwien.ac.at The Journal of Biological Chemistry 2009 imex curation Only protein-protein interactions imex curation stom_human Stomatin STOM BND7 EPB72 Protein 7.2b Erythrocyte band 7 integral membrane protein Erythrocyte membrane protein band 7.2 protein protein human Homo sapiens Human MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGRILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNADSATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAEAEASREARAKVIAAEGEMNASRALKEASMVITESPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGIIGAKHSHLG stml1_human Stomatin-like protein 1 SLP1 UNC24 EPB72-like protein 1 Protein unc-24 homolog Stomatin-related protein MSTP019 STOML1 protein protein human Homo sapiens Human MLGRSGYRALPLGDFDRFQQSSFGFLGSQKGCLSPERGGVGTGADVPQSWPSCLCHGLISFLGFLLLLVTFPISGWFALKIVPTYERMIVFRLGRIRTPQGPGMVLLLPFIDSFQRVDLRTRAFNVPPCKLASKDGAVLSVGADVQFRIWDPVLSVMTVKDLNTATRMTAQNAMTKALLKRPLREIQMEKLKISDQLLLEINDVTRAWGLEVDRVELAVEAVLQPPQDSPAGPNLDSTLQQLALHFLGGSMNSMAGGAPSPGPADTVEMVSEVEPPAPQVGARSSPKQPLAEGLLTALQPFLSEALVSQVGACYQFNVVLPSGTQSAYFLDLTTGRGRVGHGVPDGIPDVVVEMAEADLRALLCRELRPLGAYMSGRLKVKGDLAMAMKLEAVLRALK stom-stoml1-1 3316121 N/A 3316125 unspecified role unspecified role neutral component neutral component fixed cell fixed cell naturally occurring naturally occurring endogenous endogenous N/A 3316126 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 4A stom-stoml1-2 3316122 N/A 3316125 unspecified role unspecified role bait bait naturally occurring naturally occurring endogenous endogenous N/A 3316126 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5A stom-stoml1-4 3316123 N/A 3316125 unspecified role unspecified role neutral component neutral component naturally occurring naturally occurring endogenous endogenous N/A 3316126 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Figure six, Lanes 1+2 and 6-9, Supp fig 3 stom-stoml1-3 3316124 N/A 3316125 unspecified role unspecified role prey prey naturally occurring naturally occurring endogenous endogenous N/A 3316126 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5A-2