DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ furgason-2009-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Furgason ML., MacDonald C., Shanks SG., Ryder SP., Bryant NJ., Munson M. DIP original experiment: DIP-134445X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. Furgason ML., MacDonald C., Shanks SG., Ryder SP., Bryant NJ., Munson M. Only protein-protein interactions imex curation furgason-2009-2 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel tag fluorescence tag visualisation by fluorescence tag fluorescence Furgason ML., MacDonald C., Shanks SG., Ryder SP., Bryant NJ., Munson M. DIP original experiment: DIP-134446X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. Furgason ML., MacDonald C., Shanks SG., Ryder SP., Bryant NJ., Munson M. Only protein-protein interactions imex curation tlg2_yeast t-SNARE affecting a late Golgi compartment protein 2 YOL018C TLG2 Syntaxin TLG2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFRDRTNLFLSYRRTFPHNITFSSGKAPLGDDQDIEMGTYPMMNMSHDISARLTDERKNKHENHSDALPPIFIDIAQDVDDYLLEVRRLSEQLAKVYRKNSLPGFEDKSHDEALIEDLSFKVIQMLQKCYAVMKRLKTIYNSQFVDGKQLSREELIILDNLQKIYAEKIQTESNKFRVLQNNYLKFLNKDDLKPIRNKASAENTLLLDDEEEEAAREKREGLDIEDYSKRTLQRQQQLHDTSAEAYLRERDEEITQLARGVLEVSTIFREMQDLVVDQGTIVDRIDYNLENTVVELKSADKELNKATHYQKRTQKCKVILLLTLCVIALFFFVMLKPHGGGSGGRNNGSNKYNNDDNKTVNNSHDDGSNTHINDEESNLPSIVEVTESENDALDDLL CEBD6FE11278B1F0 vps45_yeast Vacuolar protein sorting-associated protein 45 VPS45 STT10 VPL28 YGL095C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNLFDVADFYINKIVTSQSKLSVANVNEHQRIKVLLLDKNTTPTISLCATQSELLKHEIYLVERIENEQREVSRHLRCLVYVKPTEETLQHLLRELRNPRYGEYQIFFSNIVSKSQLERLAESDDLEAVTKVEEIFQDFFILNQDLFSFDLQPREFLSNKLVWSEGGLTKCTNSLVSVLLSLKIKPDIRYEGASKICERLAKEVSYEIGKNERTFFDFPVMDSTPVLLILDRNTDPITPLLQPWTYQSMINEYIGIKRNIVDLSKVPRIDKDLEKVTLSSKQDAFFRDTMYLNFGELGDKVKQYVTTYKDKTQTNSQINSIEDIKNFIEKYPEFRKLSGNVAKHMAIVGELDRQLKIKNIWEISEIEQNLSAHDANEEDFSDLIKLLQNEAVDKYYKLKLACIYSLNNQTSSDKIRQLVEILSQQLPPEDVNFFHKFKSLFSRQDKMTQSNHDKDDILTELARRFNSRMNSKSNTAENVYMQHIPEISSLLTDLSKNALFRDRFKEIDTQGHRVIGNQQSKDIPQDVILFVIGGVTYEEARLVHDFNGTMNNRMRVVLGGTSILSTKEYMDSIRSAK 4AA50D568228228F tlg2p-vps45p-1 3195032 N/A 3195034 unspecified role unspecified role unspecified role unspecified role Region 37-318 Region 37-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3195035 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false tlg2p-vps45p-2 3195033 N/A 3195034 unspecified role unspecified role unspecified role unspecified role Region 37-318 Region 37-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Alexa488-label Alexa488-label alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3195035 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false