DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jiang-2009-13 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. DIP original experiment: DIP-219534X Nature 2009 IMEx The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. Only protein-protein interactions imex curation jiang-2009-14 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. DIP original experiment: DIP-219537X Nature 2009 IMEx The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. Only protein-protein interactions imex curation jiang-2009-15 in vitro In vitro pull down pull down weight identificat confirmation by molecular weight weight identificat Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. DIP original experiment: DIP-219539X Nature 2009 IMEx The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. Only protein-protein interactions imex curation jiang-2009-16 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. DIP original experiment: DIP-219535X Nature 2009 IMEx The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. Only protein-protein interactions imex curation jiang-2009-17 in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. DIP original experiment: DIP-219538X Nature 2009 IMEx The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. Only protein-protein interactions imex curation jiang-2009-18 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. DIP original experiment: DIP-219536X Nature 2009 IMEx The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. Only protein-protein interactions imex curation jiang-2009-19 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight identificat confirmation by molecular weight weight identificat Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. DIP original experiment: DIP-219540X Nature 2009 IMEx The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Jiang Q., Karata K., Woodgate R., Cox MM., Goodman MF. Only protein-protein interactions imex curation umuc_ecoli Protein UmuC umuC DNA polymerase V JW1173 b1184 protein protein ecoli Escherichia coli (strain K12) MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEAKALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQTGGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK p0ag11-pro_0000027305 Protein UmuD' DNA polymerase V JW1172 b1183 umuD protein protein ecoli Escherichia coli (strain K12) GFPSPAADYVEQRIDLNQLLIQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLRPTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR 25 139 reca_ecoli Protein RecA recA lexB recH rnmB umuB tif zab b2699 Recombinase A JW2669 protein protein ecoli Escherichia coli (strain K12) MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEIGDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSVDDSEGVAETNEDF umuc-umud-1 3194003 N/A 3194010 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3194011 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false reca-umuc-1 3194004 N/A 3194012 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3194010 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3194011 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false umuc-reca-1 3194005 N/A 3194012 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3194010 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3194011 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false umuc-umud-2 3194006 N/A 3194010 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3194011 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false reca-umuc-2 3194007 N/A 3194012 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3194010 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3194011 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false umuc-umud-3 3194008 N/A 3194010 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3194011 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false reca-umuc-3 3194009 N/A 3194012 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3194010 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3194011 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false