DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ luik-2009-1 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Luik P., Chew C., Aittoniemi J., Chang J., Wentworth P., Dwek RA., Biggin PC., Vénien-Bryan C., Zitzmann N. DIP original experiment: DIP-134383X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy. Luik P., Chew C., Aittoniemi J., Chang J., Wentworth P., Dwek RA., Biggin PC., Vénien-Bryan C., Zitzmann N. Only protein-protein interactions imex curation luik-2009-2 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Luik P., Chew C., Aittoniemi J., Chang J., Wentworth P., Dwek RA., Biggin PC., Vénien-Bryan C., Zitzmann N. DIP original experiment: DIP-134380X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy. Luik P., Chew C., Aittoniemi J., Chang J., Wentworth P., Dwek RA., Biggin PC., Vénien-Bryan C., Zitzmann N. Only protein-protein interactions imex curation luik-2009-3 in vitro In vitro crosslink cross-linking study crosslink weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Luik P., Chew C., Aittoniemi J., Chang J., Wentworth P., Dwek RA., Biggin PC., Vénien-Bryan C., Zitzmann N. DIP original experiment: DIP-134381X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy. Luik P., Chew C., Aittoniemi J., Chang J., Wentworth P., Dwek RA., Biggin PC., Vénien-Bryan C., Zitzmann N. Only protein-protein interactions imex curation luik-2009-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Luik P., Chew C., Aittoniemi J., Chang J., Wentworth P., Dwek RA., Biggin PC., Vénien-Bryan C., Zitzmann N. DIP original experiment: DIP-134382X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy. Luik P., Chew C., Aittoniemi J., Chang J., Wentworth P., Dwek RA., Biggin PC., Vénien-Bryan C., Zitzmann N. Only protein-protein interactions imex curation polg_hcvjf Genome polyprotein protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV MSTNPKPQRKTKRNTNRRPEDVKFPGGGQIVGGVYLLPRRGPRLGVRTTRKTSERSQPRGRRQPIPKDRRSTGKAWGKPGRPWPLYGNEGLGWAGWLLSPRGSRPSWGPTDPRHRSRNVGKVIDTLTCGFADLMGYIPVVGAPLSGAARAVAHGVRVLEDGVNYATGNLPGFPFSIFLLALLSCITVPVSAAQVKNTSSSYMVTNDCSNDSITWQLEAAVLHVPGCVPCERVGNTSRCWVPVSPNMAVRQPGALTQGLRTHIDMVVMSATFCSALYVGDLCGGVMLAAQVFIVSPQYHWFVQECNCSIYPGTITGHRMAWDMMMNWSPTATMILAYVMRVPEVIIDIVSGAHWGVMFGLAYFSMQGAWAKVIVILLLAAGVDAGTTTVGGAVARSTNVIAGVFSHGPQQNIQLINTNGSWHINRTALNCNDSLNTGFLAALFYTNRFNSSGCPGRLSACRNIEAFRIGWGTLQYEDNVTNPEDMRPYCWHYPPKPCGVVPARSVCGPVYCFTPSPVVVGTTDRRGVPTYTWGENETDVFLLNSTRPPQGSWFGCTWMNSTGFTKTCGAPPCRTRADFNASTDLLCPTDCFRKHPDATYIKCGSGPWLTPKCLVHYPYRLWHYPCTVNFTIFKIRMYVGGVEHRLTAACNFTRGDRCDLEDRDRSQLSPLLHSTTEWAILPCTYSDLPALSTGLLHLHQNIVDVQYMYGLSPAITKYVVRWEWVVLLFLLLADARVCACLWMLILLGQAEAALEKLVVLHAASAANCHGLLYFAIFFVAAWHIRGRVVPLTTYCLTGLWPFCLLLMALPRQAYAYDAPVHGQIGVGLLILITLFTLTPGYKTLLGQCLWWLCYLLTLGEAMIQEWVPPMQVRGGRDGIAWAVTIFCPGVVFDITKWLLALLGPAYLLRAALTHVPYFVRAHALIRVCALVKQLAGGRYVQVALLALGRWTGTYIYDHLTPMSDWAASGLRDLAVAVEPIIFSPMEKKVIVWGAETAACGDILHGLPVSARLGQEILLGPADGYTSKGWKLLAPITAYAQQTRGLLGAIVVSMTGRDRTEQAGEVQILSTVSQSFLGTTISGVLWTVYHGAGNKTLAGLRGPVTQMYSSAEGDLVGWPSPPGTKSLEPCKCGAVDLYLVTRNADVIPARRRGDKRGALLSPRPISTLKGSSGGPVLCPRGHVVGLFRAAVCSRGVAKSIDFIPVETLDVVTRSPTFSDNSTPPAVPQTYQVGYLHAPTGSGKSTKVPVAYAAQGYKVLVLNPSVAATLGFGAYLSKAHGINPNIRTGVRTVMTGEAITYSTYGKFLADGGCASGAYDIIICDECHAVDATSILGIGTVLDQAETAGVRLTVLATATPPGSVTTPHPDIEEVGLGREGEIPFYGRAIPLSCIKGGRHLIFCHSKKKCDELAAALRGMGLNAVAYYRGLDVSIIPAQGDVVVVATDALMTGYTGDFDSVIDCNVAVTQAVDFSLDPTFTITTQTVPQDAVSRSQRRGRTGRGRQGTYRYVSTGERASGMFDSVVLCECYDAGAAWYDLTPAETTVRLRAYFNTPGLPVCQDHLEFWEAVFTGLTHIDAHFLSQTKQAGENFAYLVAYQATVCARAKAPPPSWDAMWKCLARLKPTLAGPTPLLYRLGPITNEVTLTHPGTKYIATCMQADLEVMTSTWVLAGGVLAAVAAYCLATGCVSIIGRLHVNQRVVVAPDKEVLYEAFDEMEECASRAALIEEGQRIAEMLKSKIQGLLQQASKQAQDIQPAMQASWPKVEQFWARHMWNFISGIQYLAGLSTLPGNPAVASMMAFSAALTSPLSTSTTILLNIMGGWLASQIAPPAGATGFVVSGLVGAAVGSIGLGKVLVDILAGYGAGISGALVAFKIMSGEKPSMEDVINLLPGILSPGALVVGVICAAILRRHVGPGEGAVQWMNRLIAFASRGNHVAPTHYVTESDASQRVTQLLGSLTITSLLRRLHNWITEDCPIPCSGSWLRDVWDWVCTILTDFKNWLTSKLFPKLPGLPFISCQKGYKGVWAGTGIMTTRCPCGANISGNVRLGSMRITGPKTCMNTWQGTFPINCYTEGQCAPKPPTNYKTAIWRVAASEYAEVTQHGSYSYVTGLTTDNLKIPCQLPSPEFFSWVDGVQIHRFAPTPKPFFRDEVSFCVGLNSYAVGSQLPCEPEPDADVLRSMLTDPPHITAETAARRLARGSPPSEASSSVSQLSAPSLRATCTTHSNTYDVDMVDANLLMEGGVAQTEPESRVPVLDFLEPMAEEESDLEPSIPSECMLPRSGFPRALPAWARPDYNPPLVESWRRPDYQPPTVAGCALPPPKKAPTPPPRRRRTVGLSESTISEALQQLAIKTFGQPPSSGDAGSSTGAGAAESGGPTSPGEPAPSETGSASSMPPLEGEPGDPDLESDQVELQPPPQGGGVAPGSGSGSWSTCSEEDDTTVCCSMSYSWTGALITPCSPEEEKLPINPLSNSLLRYHNKVYCTTSKSASQRAKKVTFDRTQVLDAHYDSVLKDIKLAASKVSARLLTLEEACQLTPPHSARSKYGFGAKEVRSLSGRAVNHIKSVWKDLLEDPQTPIPTTIMAKNEVFCVDPAKGGKKPARLIVYPDLGVRVCEKMALYDITQKLPQAVMGASYGFQYSPAQRVEYLLKAWAEKKDPMGFSYDTRCFDSTVTERDIRTEESIYQACSLPEEARTAIHSLTERLYVGGPMFNSKGQTCGYRRCRASGVLTTSMGNTITCYVKALAACKAAGIVAPTMLVCGDDLVVISESQGTEEDERNLRAFTEAMTRYSAPPGDPPRPEYDLELITSCSSNVSVALGPRGRRRYYLTRDPTTPLARAAWETVRHSPINSWLGNIIQYAPTIWVRMVLMTHFFSILMVQDTLDQNLNFEMYGSVYSVNPLDLPAIIERLHGLDAFSMHTYSHHELTRVASALRKLGAPPLRVWKSRARAVRASLISRGGKAAVCGRYLFNWAVKTKLKLTPLPEARLLDLSSWFTVGAGGGDIFHSVSRARPRSLLFGLLLLFVGVGLFLLPAR polg_hcvjf-1 3193883 N/A 3193887 unspecified role unspecified role unspecified role unspecified role Region 751-813 Region 751-813 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false polg_hcvjf-2 3193884 N/A 3193887 unspecified role unspecified role unspecified role unspecified role Region 751-813 Region 751-813 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false polg_hcvjf-3 3193885 N/A 3193887 unspecified role unspecified role unspecified role unspecified role Region 751-813 Region 751-813 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false polg_hcvjf-4 3193886 N/A 3193887 unspecified role unspecified role unspecified role unspecified role Region 751-813 Region 751-813 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false