DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ shen-2009-9 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shen R., Weng C., Yu J., Xie T. DIP original experiment: DIP-260356X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary. Shen R., Weng C., Yu J., Xie T. Only protein-protein interactions imex curation shen-2009-10 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Shen R., Weng C., Yu J., Xie T. DIP original experiment: DIP-260354X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary. Shen R., Weng C., Yu J., Xie T. Only protein-protein interactions imex curation shen-2009-11 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shen R., Weng C., Yu J., Xie T. DIP original experiment: DIP-260359X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary. Shen R., Weng C., Yu J., Xie T. Only protein-protein interactions imex curation shen-2009-12 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shen R., Weng C., Yu J., Xie T. DIP original experiment: DIP-260361X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary. Shen R., Weng C., Yu J., Xie T. Only protein-protein interactions imex curation shen-2009-13 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shen R., Weng C., Yu J., Xie T. DIP original experiment: DIP-260355X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx f.1, S5 Shen R., Weng C., Yu J., Xie T. Only protein-protein interactions imex curation shen-2009-14 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shen R., Weng C., Yu J., Xie T. DIP original experiment: DIP-260357X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary. Shen R., Weng C., Yu J., Xie T. Only protein-protein interactions imex curation shen-2009-15 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shen R., Weng C., Yu J., Xie T. DIP original experiment: DIP-260360X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary. Shen R., Weng C., Yu J., Xie T. Only protein-protein interactions imex curation shen-2009-16 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shen R., Weng C., Yu J., Xie T. DIP original experiment: DIP-260358X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary. Shen R., Weng C., Yu J., Xie T. Only protein-protein interactions imex curation bam_drome Protein bag-of-marbles bam CG10422 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLNARDVCPEGNDDQQLDHNFKQMEEHLALMVEGNENEDPRKATCEYEDTNEDGATCTSGVLSEIQENFGRLRLCDVTAPLLEFHGLDCLQQIQKRSRHFAFDGSPAKKSRSGGVLVTGPKQKQLQKENVWNRKSKGSASADNIEKLPITIEKLHMIGLHGDCLEHNAVLRLMNLFRSLHDHLTADLGFSRQNSMPSDYLFDMPVKSTMPKSLNVRYQLQVLCTKVERFLVQQRRTLEANRHFDFEKYDECDKLLKGFASYLDNFKLLLKPKMRNRNGNSGSNADKFHTQRMERLLIGLRDWIKAAHLSVHVFNWEMDLEHRYSGAMTESHKSLNERAILLSGAELRAAEARGISAEDLFIAQRYKLGGPIYCVLEQHEFLSALIANPETYFPPSVVAICGPQKLGAVSMEQPSASEEEFEETEEVPSSPPRHTGRVPRFRS if4a_drome Eukaryotic initiation factor 4A eIF4A ATP-dependent RNA helicase eIF4A eIF-4a l(2L)162 CG9075 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDDRNEIPQDGPASMEPEGVIESTWHEVYDNFDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVYDMINRKVLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLEVSRCFMRDPVSILVKKEELTLEGIKQFYVNVKQENWKLGTLCDLYDTLSITQSVIFCNTRRKVDQLTQEMSIHNFTVSAMHGDMEQRDREVIMKQFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPSNRENYIHRIGRGGRFGRKGVAINFITDDDRRILKDIEQFYHTTIEEMPANIADLI bgcn_drome Benign gonial cell neoplasm protein bgcn b(2)gcn CG30170 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNHIIQDKYIPQQLLYFLAGRRCCQQFPCTFRTSEHEAFANNARSLGLRSQVVHVNGNSCVKVYKQACRHYLEEPKTLVLSSGATLNMFTLLSRKSLMGKEDLELYADLVSMKANASDLPSLHLPLPAIRPPNLRFWTEAQLNFLTAFLGHSLSDEILQSLYASRVIVYNAALCWDKSVFLPLVILDDCRNKKSNVKIMCIERQAILATYNSQRTANFFGEQLGETVGIQLPYFSAVSSSTFIIYSTAQYFLRSLTSQQFRNISHLVVNDVHLHDPYTDILLSEIRMALSSHQNLRVVLLSQMGNPKKFTDFFGEGLQLNMIKQPEVAPRVSYLNELHSCIALAGIHKGPDIYKEIPEAFRANNPRNEQMDKCLQAYGELGTDAALRPFLYAVNYDLAPVNYRHSLTGKTAVHFASELNKANHLRLLLFMGADPYIVDLFQQNAISLAAMNGNHECIDVLNSYSLHGYVVKSAKPDFVDYDLIIDIMYLLRTKPEYSPGEYSPGNILIILPTYYHIVKLNYMILSHCLTGSLQECSIFLLYDNMRNDYLQALVNASDETVKVVLATDIIESLCLKVPFKYQIDTACRLNNVYDTTSCSGDDRFEWVAKDALLRRELILQPNKGDVQCFRLISKEAYEELSETSQPSLQTMQLDKICLAVKLLSPNTIISEYLGITISPPPLINVHHAVQFLKKIDVLDDAEDVTWLGCRLMDIPVSCQLGRMLIFGILLRCLDPILTIVSSLSTADPLGIPFTEDIDNLWDRFTIYIQNSIKKERTYLSDNQFSDHFIFVRLYKEWQNRMHNRTPPLYLKDEYEFMLNGLMEQLTSIRSEIVSSLRAANLIHSRGKLSMNNLNQMSCNWHMVKAALTGGMYPNIYAVDTRKSSLKSAFSGNVSMHPNTVLRDFLEPLNISAQSFRTPWIVCNRQKSHIVYATLVVPLAVAMFSGHPRIRLSPICDSEMSLTDRNVNVFIDEWIWMVMSKATAEMVMRTRYYFFKMYHDLLKHCSELDMWRRDCEPVSQYTVLTDTLSKIFESEDGFVGFFKPPPITFLPTPQLPSLYLLSVNAHFSWAREVEENMLSKPHHFNSHFIERQFFVLYAGGDCEEFHSRNTPAFIESVLGKFVRPIDTPNRHIFVILYRKDPDMMLSISRAKFVNGVFMLQEYFRNNIPVFEILDACVSLNVQTPVFDGRLMSALIDKRVGNLIMELFAFRHHWIHKR eif_4a-bam-1 3193389 N/A 3193397 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3193398 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false bam-eif_4a-1 3193390 N/A 3193398 unspecified role unspecified role prey prey Region 305-403 Region 305-403 sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3193397 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false bam-bgcn-4 3193391 N/A 3193399 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3193397 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false bam-bgcn-5 3193392 N/A 3193397 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3193399 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false bam-eif_4a-2 3193393 N/A 3193398 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3193397 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false bgcn-bam-4 3193394 N/A 3193397 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3193399 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false bam-eif_4a-3 3193395 N/A 3193398 unspecified role unspecified role prey prey Region 300-403 Region 300-403 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 150-300 Region 150-300 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3193397 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false bgcn-bam-5 3193396 N/A 3193397 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3193399 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false