DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yu-2009-7 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260345X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-8 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260353X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-9 ecolx Escherichia coli sls static light scattering sls predetermined predetermined participant predetermined Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260343X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-10 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260347X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-11 ecolx Escherichia coli sls static light scattering sls predetermined predetermined participant predetermined Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260350X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-12 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260344X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx f.4 Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-13 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260346X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-14 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260351X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-15 human Homo sapiens Human comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel anti tag western anti tag western blot anti tag western Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260348X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-16 human Homo sapiens Human crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260349X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation yu-2009-17 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. DIP original experiment: DIP-260352X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y., Ulbrich MH., Li MH., Buraei Z., Chen XZ., Ong AC., Tong L., Isacoff EY., Yang J. Only protein-protein interactions imex curation q13563-1 Polycystin-2 PKD2 Autosomal dominant polycystic kidney disease type II protein Polycystic kidney disease 2 protein Polycystwin R48321 TRPP2 Transient receptor potential cation channel subfamily P member 2 protein protein human Homo sapiens Human MVNSSRVQPQQPGDAKRPPAPRAPDPGRLMAGCAAVGASLAAPGGLCEQRGLEIEMQRIRQAAARDPPAGAAASPSPPLSSCSRQAWSRDNPGFEAEEEEEEVEGEEGGMVVEMDVEWRPGSRRSAASSAVSSVGARSRGLGGYHGAGHPSGRRRRREDQGPPCPSPVGGGDPLHRHLPLEGQPPRVAWAERLVRGLRGLWGTRLMEESSTNREKYLKSVLRELVTYLLFLIVLCILTYGMMSSNVYYYTRMMSQLFLDTPVSKTEKTNFKTLSSMEDFWKFTEGSLLDGLYWKMQPSNQTEADNRSFIFYENLLLGVPRIRQLRVRNGSCSIPQDLRDEIKECYDVYSVSSEDRAPFGPRNGTAWIYTSEKDLNGSSHWGIIATYSGAGYYLDLSRTREETAAQVASLKKNVWLDRGTRATFIDFSVYNANINLFCVVRLLVEFPATGGVIPSWQFQPLKLIRYVTTFDFFLAACEIIFCFFIFYYVVEEILEIRIHKLHYFRSFWNCLDVVIVVLSVVAIGINIYRTSNVEVLLQFLEDQNTFPNFEHLAYWQIQFNNIAAVTVFFVWIKLFKFINFNRTMSQLSTTMSRCAKDLFGFAIMFFIIFLAYAQLAYLVFGTQVDDFSTFQECIFTQFRIILGDINFAEIEEANRVLGPIYFTTFVFFMFFILLNMFLAIINDTYSEVKSDLAQQKAEMELSDLIRKGYHKALVKLKLKKNTVDDISESLRQGGGKLNFDELRQDLKGKGHTDAEIEAIFTKYDQDGDQELTEHEHQQMRDDLEKEREDLDLDHSSLPRPMSSRSFPRSLDDSEEDDDEDSGHSSRRRGSISSGVSYEEFQVLVRRVDRMEHSIGSIVSKIDAVIVKLEIMERAKLKRREVLGRLLDGVAEDERLGRDSEIHREQMERLVREELERWESDDAASQISHGLGTPVGLNGQPRPRSSRPSSSQSTEGMEGAGGNGSSNVHV pkd1_human Polycystin-1 PKD1 Autosomal dominant polycystic kidney disease 1 protein protein protein human Homo sapiens Human MPPAAPARLALALGLGLWLGALAGGPGRGCGPCEPPCLCGPAPGAACRVNCSGRGLRTLGPALRIPADATALDVSHNLLRALDVGLLANLSALAELDISNNKISTLEEGIFANLFNLSEINLSGNPFECDCGLAWLPRWAEEQQVRVVQPEAATCAGPGSLAGQPLLGIPLLDSGCGEEYVACLPDNSSGTVAAVSFSAAHEGLLQPEACSAFCFSTGQGLAALSEQGWCLCGAAQPSSASFACLSLCSGPPPPPAPTCRGPTLLQHVFPASPGATLVGPHGPLASGQLAAFHIAAPLPVTATRWDFGDGSAEVDAAGPAASHRYVLPGRYHVTAVLALGAGSALLGTDVQVEAAPAALELVCPSSVQSDESLDLSIQNRGGSGLEAAYSIVALGEEPARAVHPLCPSDTEIFPGNGHCYRLVVEKAAWLQAQEQCQAWAGAALAMVDSPAVQRFLVSRVTRSLDVWIGFSTVQGVEVGPAPQGEAFSLESCQNWLPGEPHPATAEHCVRLGPTGWCNTDLCSAPHSYVCELQPGGPVQDAENLLVGAPSGDLQGPLTPLAQQDGLSAPHEPVEVMVFPGLRLSREAFLTTAEFGTQELRRPAQLRLQVYRLLSTAGTPENGSEPESRSPDNRTQLAPACMPGGRWCPGANICLPLDASCHPQACANGCTSGPGLPGAPYALWREFLFSVPAGPPAQYSVTLHGQDVLMLPGDLVGLQHDAGPGALLHCSPAPGHPGPRAPYLSANASSWLPHLPAQLEGTWACPACALRLLAATEQLTVLLGLRPNPGLRLPGRYEVRAEVGNGVSRHNLSCSFDVVSPVAGLRVIYPAPRDGRLYVPTNGSALVLQVDSGANATATARWPGGSVSARFENVCPALVATFVPGCPWETNDTLFSVVALPWLSEGEHVVDVVVENSASRANLSLRVTAEEPICGLRATPSPEARVLQGVLVRYSPVVEAGSDMVFRWTINDKQSLTFQNVVFNVIYQSAAVFKLSLTASNHVSNVTVNYNVTVERMNRMQGLQVSTVPAVLSPNATLALTAGVLVDSAVEVAFLWTFGDGEQALHQFQPPYNESFPVPDPSVAQVLVEHNVMHTYAAPGEYLLTVLASNAFENLTQQVPVSVRASLPSVAVGVSDGVLVAGRPVTFYPHPLPSPGGVLYTWDFGDGSPVLTQSQPAANHTYASRGTYHVRLEVNNTVSGAAAQADVRVFEELRGLSVDMSLAVEQGAPVVVSAAVQTGDNITWTFDMGDGTVLSGPEATVEHVYLRAQNCTVTVGAASPAGHLARSLHVLVFVLEVLRVEPAACIPTQPDARLTAYVTGNPAHYLFDWTFGDGSSNTTVRGCPTVTHNFTRSGTFPLALVLSSRVNRAHYFTSICVEPEVGNVTLQPERQFVQLGDEAWLVACAWPPFPYRYTWDFGTEEAAPTRARGPEVTFIYRDPGSYLVTVTASNNISAANDSALVEVQEPVLVTSIKVNGSLGLELQQPYLFSAVGRGRPASYLWDLGDGGWLEGPEVTHAYNSTGDFTVRVAGWNEVSRSEAWLNVTVKRRVRGLVVNASRTVVPLNGSVSFSTSLEAGSDVRYSWVLCDRCTPIPGGPTISYTFRSVGTFNIIVTAENEVGSAQDSIFVYVLQLIEGLQVVGGGRYFPTNHTVQLQAVVRDGTNVSYSWTAWRDRGPALAGSGKGFSLTVLEAGTYHVQLRATNMLGSAWADCTMDFVEPVGWLMVAASPNPAAVNTSVTLSAELAGGSGVVYTWSLEEGLSWETSEPFTTHSFPTPGLHLVTMTAGNPLGSANATVEVDVQVPVSGLSIRASEPGGSFVAAGSSVPFWGQLATGTNVSWCWAVPGGSSKRGPHVTMVFPDAGTFSIRLNASNAVSWVSATYNLTAEEPIVGLVLWASSKVVAPGQLVHFQILLAAGSAVTFRLQVGGANPEVLPGPRFSHSFPRVGDHVVSVRGKNHVSWAQAQVRIVVLEAVSGLQVPNCCEPGIATGTERNFTARVQRGSRVAYAWYFSLQKVQGDSLVILSGRDVTYTPVAAGLLEIQVRAFNALGSENRTLVLEVQDAVQYVALQSGPCFTNRSAQFEAATSPSPRRVAYHWDFGDGSPGQDTDEPRAEHSYLRPGDYRVQVNASNLVSFFVAQATVTVQVLACREPEVDVVLPLQVLMRRSQRNYLEAHVDLRDCVTYQTEYRWEVYRTASCQRPGRPARVALPGVDVSRPRLVLPRLALPVGHYCFVFVVSFGDTPLTQSIQANVTVAPERLVPIIEGGSYRVWSDTRDLVLDGSESYDPNLEDGDQTPLSFHWACVASTQREAGGCALNFGPRGSSTVTIPRERLAAGVEYTFSLTVWKAGRKEEATNQTVLIRSGRVPIVSLECVSCKAQAVYEVSRSSYVYLEGRCLNCSSGSKRGRWAARTFSNKTLVLDETTTSTGSAGMRLVLRRGVLRDGEGYTFTLTVLGRSGEEEGCASIRLSPNRPPLGGSCRLFPLGAVHALTTKVHFECTGWHDAEDAGAPLVYALLLRRCRQGHCEEFCVYKGSLSSYGAVLPPGFRPHFEVGLAVVVQDQLGAAVVALNRSLAITLPEPNGSATGLTVWLHGLTASVLPGLLRQADPQHVIEYSLALVTVLNEYERALDVAAEPKHERQHRAQIRKNITETLVSLRVHTVDDIQQIAAALAQCMGPSRELVCRSCLKQTLHKLEAMMLILQAETTAGTVTPTAIGDSILNITGDLIHLASSDVRAPQPSELGAESPSRMVASQAYNLTSALMRILMRSRVLNEEPLTLAGEEIVAQGKRSDPRSLLCYGGAPGPGCHFSIPEAFSGALANLSDVVQLIFLVDSNPFPFGYISNYTVSTKVASMAFQTQAGAQIPIERLASERAITVKVPNNSDWAARGHRSSANSANSVVVQPQASVGAVVTLDSSNPAAGLHLQLNYTLLDGHYLSEEPEPYLAVYLHSEPRPNEHNCSASRRIRPESLQGADHRPYTFFISPGSRDPAGSYHLNLSSHFRWSALQVSVGLYTSLCQYFSEEDMVWRTEGLLPLEETSPRQAVCLTRHLTAFGASLFVPPSHVRFVFPEPTADVNYIVMLTCAVCLVTYMVMAAILHKLDQLDASRGRAIPFCGQRGRFKYEILVKTGWGRGSGTTAHVGIMLYGVDSRSGHRHLDGDRAFHRNSLDIFRIATPHSLGSVWKIRVWHDNKGLSPAWFLQHVIVRDLQTARSAFFLVNDWLSVETEANGGLVEKEVLAASDAALLRFRRLLVAELQRGFFDKHIWLSIWDRPPRSRFTRIQRATCCVLLICLFLGANAVWYGAVGDSAYSTGHVSRLSPLSVDTVAVGLVSSVVVYPVYLAILFLFRMSRSKVAGSPSPTPAGQQVLDIDSCLDSSVLDSSFLTFSGLHAEQAFVGQMKSDLFLDDSKSLVCWPSGEGTLSWPDLLSDPSIVGSNLRQLARGQAGHGLGPEEDGFSLASPYSPAKSFSASDEDLIQQVLAEGVSSPAPTQDTHMETDLLSSLSSTPGEKTETLALQRLGELGPPSPGLNWEQPQAARLSRTGLVEGLRKRLLPAWCASLAHGLSLLLVAVAVAVSGWVGASFPPGVSVAWLLSSSASFLASFLGWEPLKVLLEALYFSLVAKRLHPDEDDTLVESPAVTPVSARVPRVRPPHGFALFLAKEEARKVKRLHGMLRSLLVYMLFLLVTLLASYGDASCHGHAYRLQSAIKQELHSRAFLAITRSEELWPWMAHVLLPYVHGNQSSPELGPPRLRQVRLQEALYPDPPGPRVHTCSAAGGFSTSDYDVGWESPHNGSGTWAYSAPDLLGAWSWGSCAVYDSGGYVQELGLSLEESRDRLRFLQLHNWLDNRSRAVFLELTRYSPAVGLHAAVTLRLEFPAAGRALAALSVRPFALRRLSAGLSLPLLTSVCLLLFAVHFAVAEARTWHREGRWRVLRLGAWARWLLVALTAATALVRLAQLGAADRQWTRFVRGRPRRFTSFDQVAQLSSAARGLAASLLFLLLVKAAQQLRFVRQWSVFGKTLCRALPELLGVTLGLVVLGVAYAQLAILLVSSCVDSLWSVAQALLVLCPGTGLSTLCPAESWHLSPLLCVGLWALRLWGALRLGAVILRWRYHALRGELYRPAWEPQDYEMVELFLRRLRLWMGLSKVKEFRHKVRFEGMEPLPSRSSRGSKVSPDVPPPSAGSDASHPSTSSSQLDGLSVSLGRLGTRCEPEPSRLQAVFEALLTQFDRLNQATEDVYQLEQQLHSLQGRRSSRAPAGSSRGPSPGLRPALPSRLARASRGVDLATGPSRTPLRAKNKVHPSST pkd1-pkd2-5 3193327 N/A 3193338 unspecified role unspecified role prey prey Region 833-895 Region 833-895 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q13563-1:p.[Leu842Ala;Val846Ala;Met849Ala;Ile860Ala;Val863Ala;Leu867Ala] L842A/V846A/M849A/I860A/V863A/L867A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3193339 unspecified role unspecified role bait bait Region 4213-4248 Region 4213-4248 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false pkd1-pkd2-6 3193328 N/A 3193338 unspecified role unspecified role prey prey Q13563-1:p.[Leu842Ala;Val846Ala;Met849Ala;Ile860Ala;Val863Ala;Leu867Ala] L842A/V846A/M849A/I860A/V863A/L867A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3193339 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false pkd1-pkd2-7 3193329 N/A 3193339 unspecified role unspecified role unspecified role unspecified role Region 4191-4279 Region 4191-4279 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3193338 unspecified role unspecified role unspecified role unspecified role Region 821-926 Region 821-926 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pkd2-8 3193330 N/A 3193338 unspecified role unspecified role unspecified role unspecified role Region 833-895 Region 833-895 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pkd2-4 3193331 N/A 3193338 unspecified role unspecified role unspecified role unspecified role Region 821-926 Region 821-926 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pkd1-pkd2-8 3193332 N/A 3193339 unspecified role unspecified role unspecified role unspecified role Region 4191-4279 Region 4191-4279 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3193338 unspecified role unspecified role unspecified role unspecified role Region 821-926 Region 821-926 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pkd2-pkd1-3 3193333 N/A 3193338 unspecified role unspecified role bait bait Q13563-1:p.[Leu842Ala;Val846Ala;Met849Ala;Ile860Ala;Val863Ala;Leu867Ala] L842A/V846A/M849A/I860A/V863A/L867A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3193339 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false pkd2-5 3193334 N/A 3193338 unspecified role unspecified role unspecified role unspecified role Region 821-926 Region 821-926 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q13563-1:p.[Leu842Ala;Val846Ala;Met849Ala;Ile860Ala;Val863Ala;Leu867Ala] L842A/V846A/M849A/I860A/V863A/L867A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pkd2-6 3193335 N/A 3193338 unspecified role unspecified role unspecified role unspecified role HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false pkd2-11 3193336 N/A 3193338 unspecified role unspecified role unspecified role unspecified role HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13563-1:p.[Leu842Ala;Val846Ala;Met849Ala;Ile860Ala;Val863Ala;Leu867Ala] L842A/V846A/M849A/I860A/V863A/L867A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false pkd2-12 3193337 N/A 3193338 unspecified role unspecified role unspecified role unspecified role Region 833-872 Region 833-872 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false