DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ agarwal-2009-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. DIP original experiment: DIP-133090X Nature structural & molecular biology 2009 IMEx Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. Only protein-protein interactions imex curation agarwal-2009-2 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. DIP original experiment: DIP-133092X Nature structural & molecular biology 2009 IMEx Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. Only protein-protein interactions imex curation agarwal-2009-3 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. DIP original experiment: DIP-133091X Nature structural & molecular biology 2009 IMEx Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. Only protein-protein interactions imex curation vamp2_human Vesicle-associated membrane protein 2 Synaptobrevin-2 VAMP2 SYB2 protein protein human Homo sapiens Human MSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST q57236_clobo F bonT protein protein clobo Clostridium botulinum MPVVINSFNYNDPVNDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTDPSDFDPPASLENGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINSNPAGEVLLQEISYAKPYLGNEHTPINEFHPVTRTTSVNIKSSTNVKSSIILNLLVLGAGPDIFENSSYPVRKLMDSGGVYDPSNDGFGSINIVTFSPEYEYTFNDISGGYNSSTESFIADPAISLAHELIHALHGLYGARGVTYKETIKVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSRVNSAPPEYDINEYKDYFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNIGNLAVNNRGQNIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNRELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSETIPQISNQTLNTLVQDDSYVPRYDSNGTSEIEEHNVVDLNVFFYLHAQKVPEGETNISLTSSIDTALSEESQVYTFFSSEFINTINKPVHAALFISWINQVIRDFTTEATQKSTFDKIADISLVVPYVGLALNIGNEVQKENFKEAFELLGAGILLEFVPELLIPTILVFTIKSFIGSSENKNKIIKAINNSLMERETKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNNYTSDERNRLESEYNINNIREELNKKVSLAMENIERFITESSIFYLMKLINEAKVSKLREYDEGVKEYLLDYISEHRSILGNSVQELNDLVTSTLNNSIPFELSSYTNDKILILYFNKLYKKIKDNSILDMRYENNKFIDISGYGSNISINGDVYIYSTNRNQFGIYSSKPSEVNIAQNNDIIYNGRYQNFSISFWVRIPKYFNKVNLNNEYTIIDCIRNNNSGWKISLNYNKIIWTLQDTAGNNQKLVFNYTQMISISDYINKWIFVTITNNRLGNSRIYINGNLIDEKSISNLGDIHVSDNILFKIVGCNDTRYVGIRYFKVFDTELGKTEIETLYSDEPDPSILKDFWGNYLLYNKRYYLLNLLRTDKSITQNSNFLNINQQRGVYQKPNIFSNTRLYTGVEVIIRKNGSTDISNTDNFVRKNDLAYINVVDRDVEYRLYADISIAKPEKIIKLIRTSNSNNSLGQIIVMDSIGNNCTMNFQNNNGGNIGLLGFHSNNLVASSWYYNNIRKNTSSNGCFWSFISKEHGWQEN bont-vamp2-1 3193177 N/A 3193180 unspecified role unspecified role unspecified role unspecified role Region 27-58 Region 27-58 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3193181 unspecified role unspecified role unspecified role unspecified role Region 1-416 Region 1-416 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bont-vamp2-2 3193178 N/A 3193180 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-97 Region 1-97 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P63027:p.[Val53Asp;Leu54Asp] V53D/L54D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P63027:p.Val53Asp V53D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P63027:p.Val50Asp V50D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P63027:p.Glu41Ala E41A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3193181 enzyme enzyme unspecified role unspecified role Region 1-416 Region 1-416 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli protein cleavage protein cleavage false false false bont-vamp2-3 3193179 N/A 3193180 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-94 Region 1-94 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3193181 enzyme enzyme unspecified role unspecified role Region 1-416 Region 1-416 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q57236:p.Arg133Ala R133A/K mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q57236:p.Glu164Ala E164A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q57236:p.Arg171Ala R171A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q57236:p.Arg133Lys mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli protein cleavage protein cleavage false false false