DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ bajorek-2009-1 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. DIP original experiment: DIP-133082X Nature structural & molecular biology 2009 IMEx Structural basis for ESCRT-III protein autoinhibition. Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. Only protein-protein interactions imex curation bajorek-2009-2 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. DIP original experiment: DIP-133085X Nature structural & molecular biology 2009 IMEx Structural basis for ESCRT-III protein autoinhibition. Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. Only protein-protein interactions imex curation bajorek-2009-3 in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. DIP original experiment: DIP-133084X Nature structural & molecular biology 2009 IMEx Structural basis for ESCRT-III protein autoinhibition. Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. Only protein-protein interactions imex curation bajorek-2009-4 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. DIP original experiment: DIP-133086X Nature structural & molecular biology 2009 IMEx Structural basis for ESCRT-III protein autoinhibition. Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. Only protein-protein interactions imex curation bajorek-2009-5 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. DIP original experiment: DIP-133083X Nature structural & molecular biology 2009 IMEx Structural basis for ESCRT-III protein autoinhibition. Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. Only protein-protein interactions imex curation bajorek-2009-6 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. DIP original experiment: DIP-133087X Nature structural & molecular biology 2009 IMEx Structural basis for ESCRT-III protein autoinhibition. Bajorek M., Schubert HL., McCullough J., Langelier C., Eckert DM., Stubblefield WM., Uter NT., Myszka DG., Hill CP., Sundquist WI. Only protein-protein interactions imex curation chm1b_human Charged multivesicular body protein 1b CHMP1B C18orf2 Chromatin-modifying protein 1b CHMP1.5 Vacuolar protein sorting-associated protein 46-2 protein protein human Homo sapiens Human MSNMEKHLFNLKFAAKELSRSAKKCDKEEKAEKAKIKKAIQKGNMEVARIHAENAIRQKNQAVNFLRMSARVDAVAARVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFETLDVQTQQMEDTMSSTTTLTTPQNQVDMLLQEMADEAGLDLNMELPQGQTGSVGTSVASAEQDELSQRLARLRDQV p53990-4 IST1 homolog IST1 KIAA0174 Putative MAPK-activating protein PM28 protein protein human Homo sapiens Human MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFEELKKKT chm2a_human Charged multivesicular body protein 2a BC2 CHMP2 Chromatin-modifying protein 2a Putative breast adenocarcinoma marker BC-2 Vacuolar protein sorting-associated protein 2-1 CHMP2A protein protein human Homo sapiens Human MDLLFGRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKMAKQGQMDAVRIMAKDLVRTRRYVRKFVLMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMGTMNRQLKLPQIQKIMMEFERQAEIMDMKEEMMNDAIDDAMGDEEDEEESDAVVSQVLDELGLSLTDELSNLPSTGGSLSVAAGGKKAEAAASALADADADLEERLKNLRRD q9y3e7-1 Charged multivesicular body protein 3 CHMP3 CGI149 NEDF VPS24 Chromatin-modifying protein 3 Neuroendocrine differentiation factor Vacuolar protein sorting-associated protein 24 CGI-149 Vps24alpha protein protein human Homo sapiens Human MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS ist1-chmp1b-1 3192777 N/A 3192783 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3192784 unspecified role unspecified role bait bait Region 1-189 Region 1-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Tyr64Asp Y64D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Tyr165Ala Y165A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Glu168Arg E168R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Asp63Ala D63A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Glu67Ala E67A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Arg164Asp R164D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ist1-1 3192778 N/A 3192784 unspecified role unspecified role unspecified role unspecified role Region 1-189 Region 1-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Tyr43Asp Y43D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Arg51Asp R51D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Ile54Asp I54D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ist1-2 3192779 N/A 3192784 unspecified role unspecified role unspecified role unspecified role Region 1-189 Region 1-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Tyr43Asp Y43D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Arg51Asp R51D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P53990-4:p.Arg51Asp R51D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false chmp1b-1 3192780 N/A 3192783 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false chmp3-chmp2a-1 3192781 N/A 3192785 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3192786 unspecified role unspecified role bait bait Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3E7-1:p.[Val59Asp;Val62Asp] V59D/V62D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9Y3E7-1:p.[Val59Asp;Val62Asp;Ile168Asp;Leu169Asp] V59D/V62D/I168D/L169D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false chmp2a-chmp3-3 3192782 N/A 3192785 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3192786 unspecified role unspecified role unspecified role unspecified role Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false