DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ roosild-2009-1 ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel anti tag western anti tag western blot anti tag western Roosild TP., Castronovo S., Miller S., Li C., Rasmussen T., Bartlett W., Gunasekera B., Choe S., Booth IR. DIP original experiment: DIP-132059X Structure (London, England : 1993) 2009 IMEx KTN (RCK) domains regulate K+ channels and transporters by controlling the dimer-hinge conformation. Roosild TP., Castronovo S., Miller S., Li C., Rasmussen T., Bartlett W., Gunasekera B., Choe S., Booth IR. Only protein-protein interactions imex curation roosild-2009-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Roosild TP., Castronovo S., Miller S., Li C., Rasmussen T., Bartlett W., Gunasekera B., Choe S., Booth IR. DIP original experiment: DIP-132059X Structure (London, England : 1993) 2009 IMEx KTN (RCK) domains regulate K+ channels and transporters by controlling the dimer-hinge conformation. Roosild TP., Castronovo S., Miller S., Li C., Rasmussen T., Bartlett W., Gunasekera B., Choe S., Booth IR. Only protein-protein interactions imex curation kefc_ecoli Glutathione-regulated potassium-efflux system protein KefC kefC trkC b0047 K(+)/H(+) antiporter JW0046 protein protein ecoli Escherichia coli (strain K12) MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPWGLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGCGALQMVICGGLLGLFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAIPLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREVFSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLFFIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQGSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREADEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDATRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQAGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTKARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS keff_ecoli Glutathione-regulated potassium-efflux system ancillary protein KefF yabF kefF b0046 JW0045 Quinone oxidoreductase KefF protein protein ecoli Escherichia coli (strain K12) MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQARHYKQRLLEWQEAHHG F641E3952F4EFC41 kefc-1 3192710 N/A 3192712 unspecified role unspecified role neutral component neutral component His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false kefc-keff-1 3192710 N/A 3192713 unspecified role unspecified role neutral component neutral component purified purified ecolx Escherichia coli Stoichiometry: 2.0 N/A 3192712 unspecified role unspecified role neutral component neutral component purified purified His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2B kefc-keff-2 3192711 N/A 3192713 unspecified role unspecified role neutral component neutral component purified purified ecolx Escherichia coli Stoichiometry: 2.0 N/A 3192712 unspecified role unspecified role neutral component neutral component purified purified His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Table1