DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ertrk_hasdemir-2009-1 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ertürk-Hasdemir D., Broemer M., Leulier F., Lane WS., Paquette N., Hwang D., Kim CH., Stöven S., Meier P., Silverman N. DIP original experiment: DIP-260328X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Two roles for the Drosophila IKK complex in the activation of Relish and the induction of antimicrobial peptide genes. Ertürk-Hasdemir D., Broemer M., Leulier F., Lane WS., Paquette N., Hwang D., Kim CH., Stöven S., Meier P., Silverman N. Only protein-protein interactions imex curation ertrk_hasdemir-2009-2 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Ertürk-Hasdemir D., Broemer M., Leulier F., Lane WS., Paquette N., Hwang D., Kim CH., Stöven S., Meier P., Silverman N. DIP original experiment: DIP-260326X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Two roles for the Drosophila IKK complex in the activation of Relish and the induction of antimicrobial peptide genes. Ertürk-Hasdemir D., Broemer M., Leulier F., Lane WS., Paquette N., Hwang D., Kim CH., Stöven S., Meier P., Silverman N. Only protein-protein interactions imex curation ertrk_hasdemir-2009-3 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Ertürk-Hasdemir D., Broemer M., Leulier F., Lane WS., Paquette N., Hwang D., Kim CH., Stöven S., Meier P., Silverman N. DIP original experiment: DIP-260327X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Two roles for the Drosophila IKK complex in the activation of Relish and the induction of antimicrobial peptide genes. Ertürk-Hasdemir D., Broemer M., Leulier F., Lane WS., Paquette N., Hwang D., Kim CH., Stöven S., Meier P., Silverman N. Only protein-protein interactions imex curation ikkb_drome Inhibitor of nuclear factor kappa-B kinase subunit beta IKKbeta Cactus kinase IKK DIK IKK-like protein Immune response deficient protein 5 Lipopolysaccharide-activated kinase ird5 CG4201 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MITVVFCFSDCHGGIQILGRMSSVNKIKLNENNKMHSFGNWERCRNLGEGGFGLVIHWRNRTTGREIATKHIKEMGALSADQQVKLSERWNKELNWSRQFKNFPHIVAGVDIEDPDFLEYLNGMFSAKLPVIVLEYCNGGDVRKRLQSPENANGLTEFEVRQILGALRKALHFLHSQCGICHRDLKPDNIVIQRGVDGKKIYKLTDFGLARGTPDQTMVQSVVGTRHYYAPEVVENGFYNSTVDLWSFGVIAYELVTGELPFIPHQTLKNIILNLIKKPAKCIAITEDPEDNTRFVNQFELPQTHHLSRPWAAQFTKWLASPLNSNYKERGQLAANNVPVVFADLDKILNMNVLTIFAVNNCERLEYAVSAEMTMKDLIALIVLDTGMDEKELYFVLPTSHPHKTITPKSTPLQLYVEEWSDTSKDSRKWTKRSNPPVMLYIFQVKKECDYKIPEPILSILSRKFIANKFKTKERWLQKRVVLDMLYVLTKEQARYEMLVSGINERALSLEDEMMENSFIDSIDKQRIIISFAYDQLTSLLKEAQAKIPSRQLISSAQWEKLNRNYNFIIQSAKSIRSFLEACLREAKDMVKTTNQLRKEVCEKDLFDCARFYKKYLCNGAIISPSELNNDAEEFAKSRFKLYNEGEARHLPKSIDHMHYLYFKTKESIPVLLQQFCDIKKEIFQINLQMLMSASSTPPPKLELSAAMDRLAISSGSPSSDPFDSLRTINAIEEAERINNILVNEMKIDHY q94527-1 Nuclear factor NF-kappa-B p110 subunit C p110 Rel-p110 Relish protein CG11992 A B Rel protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNMNQYYDLDNGKNVMFMNDASSTSGYSSSTSPNSTNRSFSPAHSPKTMELQTDFANLNLPGGNSPHQPPMANSPYQNQLLNNGGICQLGATNLINSTGVSFGVANVTSFGNMYMDHQYFVPAPATVPPSQNFGYHQNGLASDGDIKHVPQLRIVEQPVEKFRFRYKSEMHGTHGSLNGANSKRTPKTFPEVTLCNYDGPAVIRCSLFQTNLDSPHSHQLVVRKDDRDVCDPHDLHVSKERGYVAQFINMGIIHTAKKYIFEELCKKKQDRLVFQMNRRELSHKQLQELHQETEREAKDMNLNQVRLCFEAFKIEDNGAWVPLAPPVYSNAINNRKSAQTGELRIVRLSKPTGGVMGNDELILLVEKVSKKNIKVRFFEEDEDGETVWEAYAKFRESDVHHQYAIVCQTPPYKDKDVDREVNVYIELIRPSDDERSFPALPFRYKPRSVIVSRKRRRTGSSANSSSSGTESSNNSLDLPKTLGLAQPPNGLPNLSQHDQTISEEFGREKHLNEFIASEDFRKLIEHNSSDLEKICQLDMGELQHDGHNRAEVPSHRNRTIKCLDDLFEIYKQDRISPIKISHHKVEKWFIEHALNNYNRDTLLHEVISHKKDKLKLAIQTIQVMNYFNLKDVVNSTLNADGDSALHVACQQDRAHYIRPLLGMGCNPNLKNNAGNTPLHVAVKEEHLSCVESFLNGVPTVQLDLSLTNDDGLTPLHMAIRQNKYDVAKKLISYDRTSISVANTMDGNNALHMAVLEQSVELLVLILDAQNENLTDILQAQNAAGHTPLELAERKANDRVVQLLKNVYPEKGELAMTWIPCKVKEEIDSSSDESSDAGQLEIKSEEMDIETKDEDSVELDLSSGPRRQKDESSRDTEMDNNKLQLLLKNKFIYDRLCSLLNQPLGHGSDPQDRKWMQLARQTHLKQFAFIWLGAEDLLDHVKRKGASVEFSTFARALQAVDPQAYALLVNPT ird5-1 3192090 N/A 3192093 putative self putative self unspecified role unspecified role Q9VEZ5:p.Lys70Ala K50A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false ird5-rel-1 3192091 N/A 3192093 enzyme enzyme unspecified role unspecified role Q9VEZ5:p.Lys70Ala K50A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3192094 enzyme target enzyme target substrate substrate unspecified role unspecified role pS528/529 pS528/529 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false ird5-rel-2 3192092 N/A 3192093 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3192094 enzyme target enzyme target substrate substrate unspecified role unspecified role pS528/529 pS528/529 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q94527-1:p.[Ser528Ala;Ser529Ala] S528A/S529A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro phosphorylation phosphorylation reaction phosphorylation false false false