DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ liang-2009-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liang B., Zhou J., Kahen E., Terns RM., Terns MP., Li H. DIP original experiment: DIP-133060X Nature structural & molecular biology 2009 IMEx Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA. Liang B., Zhou J., Kahen E., Terns RM., Terns MP., Li H. Only protein-protein interactions imex curation liang-2009-6 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Liang B., Zhou J., Kahen E., Terns RM., Terns MP., Li H. DIP original experiment: DIP-133063X Nature structural & molecular biology 2009 IMEx Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA. Liang B., Zhou J., Kahen E., Terns RM., Terns MP., Li H. Only protein-protein interactions imex curation liang-2009-7 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liang B., Zhou J., Kahen E., Terns RM., Terns MP., Li H. DIP original experiment: DIP-133061X Nature structural & molecular biology 2009 IMEx Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA. Liang B., Zhou J., Kahen E., Terns RM., Terns MP., Li H. Only protein-protein interactions imex curation liang-2009-8 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liang B., Zhou J., Kahen E., Terns RM., Terns MP., Li H. DIP original experiment: DIP-133062X Nature structural & molecular biology 2009 IMEx Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA. Liang B., Zhou J., Kahen E., Terns RM., Terns MP., Li H. Only protein-protein interactions imex curation rl7a_pyrfu 50S ribosomal protein L7Ae Ribosomal protein L8e PF1367 rpl7ae Large ribosomal subunit protein eL8 protein protein pyrfu Pyrococcus furiosus MAKPSYVKFEVPKELAEKALQAVEIARDTGKIRKGTNETTKAVERGQAKLVIIAEDVDPEEIVAHLPPLCEEKEIPYIYVPSKKELGAAAGIEVAAASVAIIEPGKARDLVEEIAMKVKELMK nop10_pyrfu Ribosome biogenesis protein Nop10 nop10 PF1141 protein protein pyrfu Pyrococcus furiosus MRFRIRKCPKCGRYTLKEVCPVCGEKTKVAHPPRFSPEDPYGEYRRRWKREVLGIGRKEK trub_pyrfu Probable tRNA pseudouridine synthase B truB tRNA pseudouridine(55) synthase tRNA pseudouridylate synthase tRNA-uridine isomerase PF1785 protein protein pyrfu Pyrococcus furiosus MARDEVRRILPADIKREVLIKDENAETNPDWGFPPEKRPIEMHIQFGVINLDKPPGPTSHEVVAWIKKILNLEKAGHGGTLDPKVSGVLPVALEKATRVVQALLPAGKEYVALMHLHGDVPEDKIIQVMKEFEGEIIQRPPLRSAVKRRLRTRKVYYIEVLEIEGRDVLFRVGVEAGTYIRSLIHHIGLALGVGAHMSELRRTRSGPFKEDETLITLHDLVDYYYFWKEDGIEEYFRKAIQPMEKAVEHLPKVWIKDSAVAAVTHGADLAVPGIAKLHAGIKRGDLVAIMTLKDELVALGKAMMTSQEMLEKTKGIAVDVEKVFMPRDWYPKLWEKRDRS cbf5-l7ae-1 3191613 N/A 3191617 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3191618 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3191619 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false nop10-cbf5-1 3191614 N/A 3191618 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3191619 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false cbf5-nop10-6 3191615 N/A 3191618 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3191619 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false cbf5-nop10-7 3191616 N/A 3191618 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3191619 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false