DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ larrea-2009-1 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. DIP original experiment: DIP-134275X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and increase cell motility. Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. Only protein-protein interactions imex curation larrea-2009-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. DIP original experiment: DIP-134270X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and increase cell motility. Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. Only protein-protein interactions imex curation larrea-2009-3 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. DIP original experiment: DIP-134273X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and increase cell motility. Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. Only protein-protein interactions imex curation larrea-2009-4 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. DIP original experiment: DIP-134271X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and increase cell motility. Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. Only protein-protein interactions imex curation larrea-2009-5 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. DIP original experiment: DIP-134276X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and increase cell motility. Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. Only protein-protein interactions imex curation larrea-2009-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. DIP original experiment: DIP-134274X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and increase cell motility. Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. Only protein-protein interactions imex curation larrea-2009-7 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. DIP original experiment: DIP-134272X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and increase cell motility. Larrea MD., Hong F., Wander SA., da Silva TG., Helfman D., Lannigan D., Smith JA., Slingerland JM. Only protein-protein interactions imex curation cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT ccnd1_human G1/S-specific cyclin-D1 CCND1 BCL1 PRAD1 PRAD1 oncogene BCL-1 oncogene B-cell lymphoma 1 protein protein protein human Homo sapiens Human MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI cdk4_human Cyclin-dependent kinase 4 CDK4 Cell division protein kinase 4 PSK-J3 protein protein human Homo sapiens Human MATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE ks6a1_human Ribosomal protein S6 kinase alpha-1 RPS6KA1 RSK1 90 kDa ribosomal protein S6 kinase 1 MAPKAPK1A Ribosomal S6 kinase 1 MAP kinase-activated protein kinase 1a protein protein human Homo sapiens Human MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL rhoa_human Transforming protein RhoA Rho cDNA clone 12 ARHA RHO12 RHOA ARH12 protein protein human Homo sapiens Human MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL ccne1_human G1/S-specific cyclin-E1 CCNE1 CCNE protein protein human Homo sapiens Human MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA cdk4-ccnd1-27 3191373 N/A 3191380 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3191381 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3191382 unspecified role unspecified role bait bait human Homo sapiens Human association association false false false cdkn1b-rps6ka1-1 3191374 N/A 3191383 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3191380 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false cdkn1b-rhoa-1 3191375 N/A 3191384 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3191380 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false rhoa-cdkn1b-1 3191376 N/A 3191380 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3191384 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false cdkn1b-rps6ka1-2 3191377 N/A 3191383 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3191380 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false ccne1-cdk2-3 3191378 N/A 3191380 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3191385 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3191386 unspecified role unspecified role bait bait human Homo sapiens Human association association false false false rhoa-cdkn1b-2 3191379 N/A 3191380 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3191384 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false