DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhang-2009-18 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang T., Li S., Zhang Y., Zhong C., Lai Z., Ding J. DIP original experiment: DIP-132106X Structure (London, England : 1993) 2009 IMEx Crystal structure of the ARL2-GTP-BART complex reveals a novel recognition and binding mode of small GTPase with effector. Zhang T., Li S., Zhang Y., Zhong C., Lai Z., Ding J. Only protein-protein interactions imex curation zhang-2009-19 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhang T., Li S., Zhang Y., Zhong C., Lai Z., Ding J. DIP original experiment: DIP-132107X Structure (London, England : 1993) 2009 IMEx Crystal structure of the ARL2-GTP-BART complex reveals a novel recognition and binding mode of small GTPase with effector. Zhang T., Li S., Zhang Y., Zhong C., Lai Z., Ding J. Only protein-protein interactions imex curation zhang-2009-20 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang T., Li S., Zhang Y., Zhong C., Lai Z., Ding J. DIP original experiment: DIP-132108X Structure (London, England : 1993) 2009 IMEx Crystal structure of the ARL2-GTP-BART complex reveals a novel recognition and binding mode of small GTPase with effector. Zhang T., Li S., Zhang Y., Zhong C., Lai Z., Ding J. Only protein-protein interactions imex curation arl2_human ADP-ribosylation factor-like protein 2 ARL2 protein protein human Homo sapiens Human MGLLTILKKMKQKERELRLLMLGLDNAGKTTILKKFNGEDIDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEERLAGATLLIFANKQDLPGALSSNAIREVLELDSIRSHHWCIQGCSAVTGENLLPGIDWLLDDISSRIFTAD ar2bp_human ADP-ribosylation factor-like protein 2-binding protein ARL2BP BART BART1 Binder of ARF2 protein 1 protein protein human Homo sapiens Human MDALEGESFALSFSSASDAEFDAVVGYLEDIIMDDEFQLLQRNFMDKYYLEFEDTEENKLIYTPIFNEYISLVEKYIEEQLLQRIPEFNMAAFTTTLQHHKDEVAGDIFDMLLTFTDFLAFKEMFLDYRAEKEGRGLDLSSGLVVTSLCKSSSLPASQNNLRH arl2-arl2bp-10 3187149 N/A 3187152 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3187153 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false arl2-arl2bp-11 3187150 N/A 3187152 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3187153 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false arl2bp-arl2-3 3187151 N/A 3187152 unspecified role unspecified role prey prey Region 2-4 Region 2-4 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P36404:p.Leu3Ala L3A/D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P36404:p.Leu4Asp L4D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P36404:p.Ile6Arg I6R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P36404:p.Leu7Asp L7D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P36404:p.Phe50Ala F50A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P36404:p.Tyr80Ala Y80A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P36404:p.Leu3Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3187153 unspecified role unspecified role bait bait Q9Y2Y0:p.Glu56Ala E56A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y2Y0:p.Glu57Ala E57A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y2Y0:p.Leu60Ala L60A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y2Y0:p.Phe109Ala F109A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y2Y0:p.Glu74Ala E74A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y2Y0:p.Asp110Ala D110A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y2Y0:p.Leu112Ala L112A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y2Y0:p.Phe115Ala F115A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false