DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ fu-2009-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134116X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation fu-2009-6 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134120X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation fu-2009-7 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134118X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation fu-2009-8 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134122X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation fu-2009-9 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134125X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation fu-2009-10 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134124X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation fu-2009-11 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134121X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation fu-2009-12 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134117X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation fu-2009-13 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134119X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation fu-2009-14 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Fu J., Bian M., Liu J., Jiang Q., Zhang C. DIP original experiment: DIP-134123X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Fu J., Bian M., Liu J., Jiang Q., Zhang C. Only protein-protein interactions imex curation tpx2_human Targeting protein for Xklp2 TPX2 C20orf1 C20orf2 DIL2 HCA519 Restricted expression proliferation-associated protein 100 Differentially expressed in cancerous and non-cancerous lung cells 2 Protein fls353 Hepatocellular carcinoma-associated antigen 519 Hepatocellular carcinoma-associated antigen 90 protein protein human Homo sapiens Human MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENKLLGKNGTGGLFQGKTPLRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSSLEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKMKVSNNKKKPEEEGSAHQDTAEKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKDDINLLPSKSSVTKICRDPQTPVLQTKHRARAVTCKSTAELEAEELEKLQQYKFKARELDPRILEGGPILPKKPPVKPPTEPIGFDLEIEKRIQERESKKKTEDEHFEFHSRPCPTKILEDVVGVPEKKVLPITVPKSPAFALKNRIRMPTKEDEEEDEPVVIKAQPVPHYGVPFKPQIPEARTVEICPFSFDSRDKERQLQKEKKIKELQKGEVPKFKALPLPHFDTINLPEKKVKNVTQIEPFCLETDRRGALKAQTWKHQLEEELRQQKEAACFKARPNTVISQEPFVPKKEKKSVAEGLSGSLVQEPFQLATEKRAKERQELEKRMAEVEAQKAQQLEEARLQEEEQKKEELARLRRELVHKANPIRKYQGLEIKSSDQPLTVPVSPKFSTRFHC This sequence has been withdrawn from Uniprot. aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS q9nqs7-1 Inner centromere protein INCENP protein protein human Homo sapiens Human MGTTAPGPIHLLELCDQKLMEFLCNMDNKDLVWLEEIQEEAERMFTREFSKEPELMPKTPSQKNRRKKRRISYVQDENRDPIRRRLSRRKSRSSQLSSRRLRSKDSVEKLATVVGENGSVLRRVTRAAAAAAAATMALAAPSSPTPESPTMLTKKPEDNHTQCQLVPVVEIGISERQNAEQHVTQLMSTEPLPRTLSPTPASATAPTSQGIPTSDEESTPKKSKARILESITVSSLMATPQDPKGQGVGTGRSASKLRIAQVSPGPRDSPAFPDSPWRERVLAPILPDNFSTPTGSRTDSQSVRHSPIAPSSPSPQVLAQKYSLVAKQESVVRRASRRLAKKTAEEPAASGRIICHSYLERLLNVEVPQKVGSEQKEPPEEAEPVAAAEPEVPENNGNNSWPHNDTEIANSTPNPKPAASSPETPSAGQQEAKTDQADGPREPPQSARRKRSYKQAVSELDEEQHLEDEELQPPRSKTPSSPCPASKVVRPLRTFLHTVQRNQMLMTPTSAPRSVMKSFIKRNTPLRMDPKCSFVEKERQRLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKAKEAAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDSTDDEAHPRKPIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH ince_human Inner centromere protein INCENP protein protein human Homo sapiens Human MGTTAPGPIHLLELCDQKLMEFLCNMDNKDLVWLEEIQEEAERMFTREFSKEPELMPKTPSQKNRRKKRRISYVQDENRDPIRRRLSRRKSRSSQLSSRRLRSKDSVEKLATVVGENGSVLRRVTRAAAAAAAATMALAAPSSPTPESPTMLTKKPEDNHTQCQLVPVVEIGISERQNAEQHVTQLMSTEPLPRTLSPTPASATAPTSQGIPTSDEESTPKKSKARILESITVSSLMATPQDPKGQGVGTGRSASKLRIAQVSPGPRDSPAFPDSPWRERVLAPILPDNFSTPTGSRTDSQSVRHSPIAPSSPSPQVLAQKYSLVAKQESVVRRASRRLAKKTAEEPAASGRIICHSYLERLLNVEVPQKVGSEQKEPPEEAEPVAAAEPEVPENNGNNSWPHNDTEIANSTPNPKPAASSPETPSAGQQEAKTDQADGPREPPQSARRKRSYKQAVSELDEEQHLEDEELQPPRSKTPSSPCPASKVVRPLRTFLHTVQRNQMLMTPTSAPRSVMKSFIKRNTPLRMDPKCSFVEKERQRLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKAKEAAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDSTDDEAHPRKPIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD aurkb_human Aurora kinase B AIRK2 Aurora- and IPL1-like midbody-associated protein 1 Aurora/IPL1-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 STK1 STK5 Aurora 1 AURKB AIK2 AIM1 ARK2 STK12 Serine/threonine-protein kinase aurora-B protein protein human Homo sapiens Human MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA aurka-tpx2-1 3186768 N/A 3186778 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186779 unspecified role unspecified role bait bait O14965:p.Gly198Asn G198N mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false aurka-incenp-1 3186769 N/A 3186780 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 826-918 Region 826-918 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3186779 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false aurkb-birc5-4 3186770 N/A 3186781 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186782 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186783 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false aurka-incenp-2 3186771 N/A 3186781 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186779 unspecified role unspecified role bait bait O14965:p.Gly198Ala G198A/V/L mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O14965:p.Gly198Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false O14965:p.Gly198Leu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false aurkb-birc5-5 3186772 N/A 3186783 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3186782 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false aurkb-incenp-2 3186773 N/A 3186783 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3186780 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 826-918 Region 826-918 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false aurka-birc5-1 3186774 N/A 3186782 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3186779 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false aurka-birc5-2 3186775 N/A 3186781 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186782 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186779 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false incenp-aurka-1 3186776 N/A 3186780 unspecified role unspecified role bait bait Region 826-918 Region 826-918 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3186779 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false incenp-aurkb-2 3186777 N/A 3186783 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3186780 unspecified role unspecified role bait bait Region 826-918 Region 826-918 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false