DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ van-2009-2 mouse Mus musculus Mouse pull down pull down western blot western blot Immuno blot Immuno blot van Essen D., Engist B., Natoli G., Saccani S. DIP original experiment: DIP-132599X PLoS biology 2009 IMEx Two modes of transcriptional activation at native promoters by NF-kappaB p65. van Essen D., Engist B., Natoli G., Saccani S. Only protein-protein interactions imex curation van-2009-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined van Essen D., Engist B., Natoli G., Saccani S. DIP original experiment: DIP-132602X PLoS biology 2009 IMEx Two modes of transcriptional activation at native promoters by NF-kappaB p65. van Essen D., Engist B., Natoli G., Saccani S. Only protein-protein interactions imex curation van-2009-4 human Homo sapiens Human bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined van Essen D., Engist B., Natoli G., Saccani S. DIP original experiment: DIP-132604X PLoS biology 2009 IMEx Two modes of transcriptional activation at native promoters by NF-kappaB p65. van Essen D., Engist B., Natoli G., Saccani S. Only protein-protein interactions imex curation van-2009-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot van Essen D., Engist B., Natoli G., Saccani S. DIP original experiment: DIP-132600X PLoS biology 2009 IMEx Two modes of transcriptional activation at native promoters by NF-kappaB p65. van Essen D., Engist B., Natoli G., Saccani S. Only protein-protein interactions imex curation van-2009-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined van Essen D., Engist B., Natoli G., Saccani S. DIP original experiment: DIP-132601X PLoS biology 2009 IMEx Two modes of transcriptional activation at native promoters by NF-kappaB p65. van Essen D., Engist B., Natoli G., Saccani S. Only protein-protein interactions imex curation van-2009-7 human Homo sapiens Human bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined van Essen D., Engist B., Natoli G., Saccani S. DIP original experiment: DIP-132603X PLoS biology 2009 IMEx Two modes of transcriptional activation at native promoters by NF-kappaB p65. van Essen D., Engist B., Natoli G., Saccani S. Only protein-protein interactions imex curation tf65_mouse Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit Rela Nfkb3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein mouse Mus musculus Mouse MDDLFPLIFPSEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGYYEADLCPDRSIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFHVPIEEQRGDYDLNAVRLCFQVTVRDPAGRPLLLTPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFNGPTEPRPPTRRIAVPTRNSTSVPKPAPQPYTFPASLSTINFDEFSPMLLPSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTPGPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSEFQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDEDFSSIADMDFSALLSQISS med16_mouse Mediator of RNA polymerase II transcription subunit 16 Mediator complex subunit 16 Thrap5 Thyroid hormone receptor-associated protein 5 Thyroid hormone receptor-associated protein complex 95 kDa component Vitamin D3 receptor-interacting protein complex 92 kDa component Med16 protein protein mouse Mus musculus Mouse MDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQDLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAAADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLARDMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAVFYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGHPLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQVLSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDRLAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGSPLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCRDEGAASEPDEGLVDECCLLPSQLLVPNLDWLPASDGLVSRLQPKQPLRLRFGRAPTLPSSTSTLQLDGLTRAPGQPKIDHLRRLHLGAYPTEECKACTRCGCVTMLKSPNKTTAVTQWEQRWIKNCLCGGLWRRVPLSCS med17_mouse Mediator of RNA polymerase II transcription subunit 17 Med17 Crsp6 Trap80 Cofactor required for Sp1 transcriptional activation subunit 6 Mediator complex subunit 17 Thyroid hormone receptor-associated protein complex 80 kDa component protein protein mouse Mus musculus Mouse MSGVRAVRISIESACEKQVQEVGLDGTETYLQPLSMSQNLARLAQRIDFSQGSGSEEEEAAGPDGDAPDWGGAGADQDDEEGLVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQSPQTLQLISKKKSLAGAAQILLKGAERLTKSVAENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDIDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQIPHIVVKNQIISQPFPSLQLSISLCHSSDDKKSQKCAAEKPGQEDHLYVLEHNLHLLIREFHKQTLSSIVMPHPASAPFGHKRMRLSGPQAFDKNEINSIQSTEGLLEKIIKQAKHIFLRSRTAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQVRVVHRDGRVIMLSHQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDSKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL med16-rela-1 3186459 N/A 3186465 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3186466 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse Mus musculus Mouse physical association physical association false false false med17-rela-1 3186460 N/A 3186465 unspecified role unspecified role prey prey Region 431-549 Region 431-549 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3186467 unspecified role unspecified role bait bait Region 1-335 Region 1-335 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false med17-rela-2 3186461 N/A 3186467 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3186465 unspecified role unspecified role unspecified role unspecified role Region 441-474/520-549 Region 441-474/520-549 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q04207:p.[Asp539Ala;Phe540Ala] D539A/F540A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q04207:p.Gly528_Ser534delinsAlaAlaAlaSerAlaAlaAla GDEDFSS(528-534)AAASAAA mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q04207:p.Ile535_Ser541delinsAlaAlaAlaSerAlaAlaAla IADMDFS(535-541)AAASAAA mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false med17-rela-3 3186462 N/A 3186467 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3186465 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false rela-med17-1 3186463 N/A 3186465 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3186467 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false rela-rela-7 3186464 N/A 3186465 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3186465 unspecified role unspecified role unspecified role unspecified role Region 1-305 Region 1-305 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false