DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hammond-2009-1 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hammond JW., Cai D., Blasius TL., Li Z., Jiang Y., Jih GT., Meyhofer E., Verhey KJ. DIP original experiment: DIP-132597X PLoS biology 2009 IMEx Mammalian Kinesin-3 motors are dimeric in vivo and move by processive motility upon release of autoinhibition. Hammond JW., Cai D., Blasius TL., Li Z., Jiang Y., Jih GT., Meyhofer E., Verhey KJ. Only protein-protein interactions imex curation hammond-2009-2 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Hammond JW., Cai D., Blasius TL., Li Z., Jiang Y., Jih GT., Meyhofer E., Verhey KJ. DIP original experiment: DIP-132598X PLoS biology 2009 IMEx Mammalian Kinesin-3 motors are dimeric in vivo and move by processive motility upon release of autoinhibition. Hammond JW., Cai D., Blasius TL., Li Z., Jiang Y., Jih GT., Meyhofer E., Verhey KJ. Only protein-protein interactions imex curation kif1a Kinesin-like protein KIF1A protein protein rat Rattus norvegicus (Rat) Rat kif1a-kif1a-4 3186444 N/A 3186446 unspecified role unspecified role prey prey Region 1-393 Region 1-393 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false mCit-tag mCit-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3186446 unspecified role unspecified role bait bait Region 1-393 Region 1-393 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false kif1a-1 3186445 N/A 3186446 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept mCit-tag mCit-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3186446 unspecified role unspecified role fluorescence donor fluorescence donor mCFP-tag mCFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey direct interaction direct interaction false false false