DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ o_keefe-2009-1 nicbe Nicotiana benthamiana molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined O'Keefe BR., Vojdani F., Buffa V., Shattock RJ., Montefiori DC., Bakke J., Mirsalis J., d'Andrea AL., Hume SD., Bratcher B., Saucedo CJ., McMahon JB., Pogue GP., Palmer KE. DIP original experiment: DIP-216059X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component. O'Keefe BR., Vojdani F., Buffa V., Shattock RJ., Montefiori DC., Bakke J., Mirsalis J., d'Andrea AL., Hume SD., Bratcher B., Saucedo CJ., McMahon JB., Pogue GP., Palmer KE. Only protein-protein interactions imex curation o_keefe-2009-2 nicbe Nicotiana benthamiana light scattering light scattering predetermined predetermined participant predetermined O'Keefe BR., Vojdani F., Buffa V., Shattock RJ., Montefiori DC., Bakke J., Mirsalis J., d'Andrea AL., Hume SD., Bratcher B., Saucedo CJ., McMahon JB., Pogue GP., Palmer KE. DIP original experiment: DIP-216060X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component. O'Keefe BR., Vojdani F., Buffa V., Shattock RJ., Montefiori DC., Bakke J., Mirsalis J., d'Andrea AL., Hume SD., Bratcher B., Saucedo CJ., McMahon JB., Pogue GP., Palmer KE. Only protein-protein interactions imex curation o_keefe-2009-3 in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined O'Keefe BR., Vojdani F., Buffa V., Shattock RJ., Montefiori DC., Bakke J., Mirsalis J., d'Andrea AL., Hume SD., Bratcher B., Saucedo CJ., McMahon JB., Pogue GP., Palmer KE. DIP original experiment: DIP-216061X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component. O'Keefe BR., Vojdani F., Buffa V., Shattock RJ., Montefiori DC., Bakke J., Mirsalis J., d'Andrea AL., Hume SD., Bratcher B., Saucedo CJ., McMahon JB., Pogue GP., Palmer KE. Only protein-protein interactions imex curation o_keefe-2009-4 in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined O'Keefe BR., Vojdani F., Buffa V., Shattock RJ., Montefiori DC., Bakke J., Mirsalis J., d'Andrea AL., Hume SD., Bratcher B., Saucedo CJ., McMahon JB., Pogue GP., Palmer KE. DIP original experiment: DIP-216062X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component. O'Keefe BR., Vojdani F., Buffa V., Shattock RJ., Montefiori DC., Bakke J., Mirsalis J., d'Andrea AL., Hume SD., Bratcher B., Saucedo CJ., McMahon JB., Pogue GP., Palmer KE. Only protein-protein interactions imex curation grfin_grisq Griffithsin protein protein grisq Griffithsia sp. (strain Q66D336) SLTHRKFGGSGGSPFSGLSSIAVRSGSYLDXIIIDGVHHGGSGGNLSPTFTFGSGEYISNMTIRSGDYIDNISFETNMGRRFGPYGGSGGSANTLSNVKVIQINGSAGDYLDSLDIYYEQY mint gp120 Envelope surface glycoprotein gp120 protein protein 9hiv1 Human immunodeficiency virus 1 grfin_grisq-2 3186428 N/A 3186432 unspecified role unspecified role unspecified role unspecified role nicbe Nicotiana benthamiana direct interaction direct interaction false false false grfin_grisq-3 3186429 N/A 3186432 unspecified role unspecified role unspecified role unspecified role nicbe Nicotiana benthamiana direct interaction direct interaction false false false gp120-grfin_grisq-1 3186430 N/A 3186432 unspecified role unspecified role prey prey nicbe Nicotiana benthamiana N/A 3186433 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false gp120-grfin_grisq-2 3186431 N/A 3186432 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3186433 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false