DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ puxeddu-2009-1 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134065X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134074X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-3 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134068X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-4 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134072X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-5 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134070X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134066X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-7 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134069X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-8 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134073X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-9 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134071X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-10 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134075X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation puxeddu-2009-11 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. DIP original experiment: DIP-134067X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E., Uhart M., Li CC., Ahmad F., Pacheco-Rodriguez G., Manganiello VC., Moss J., Vaughan M. Only protein-protein interactions imex curation big2_human Brefeldin A-inhibited guanine nucleotide-exchange protein 2 ARFGEF2 ARFGEP2 BIG2 ADP-ribosylation factor guanine nucleotide-exchange factor 2 protein protein human Homo sapiens Human MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW big1_human Brefeldin A-inhibited guanine nucleotide-exchange protein 1 ARFGEF1 ARFGEP1 BIG1 p200 ARF-GEP1 p200 ARF guanine nucleotide exchange factor ADP-ribosylation factor guanine nucleotide-exchange factor 1 protein protein human Homo sapiens Human MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ kap0_human cAMP-dependent protein kinase type I-alpha regulatory subunit Tissue-specific extinguisher 1 PRKAR1A PKR1 PRKAR1 TSE1 protein protein human Homo sapiens Human MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV pde3a_human cGMP-inhibited 3',5'-cyclic phosphodiesterase A PDE3A Cyclic GMP-inhibited phosphodiesterase A protein protein human Homo sapiens Human MAVPGDAARVRDKPVHSGVSQAPTAGRDCHHRADPASPRDSGCRGCWGDLVLQPLRSSRKLSSALCAGSLSFLLALLVRLVRGEVGCDLEQCKEAAAAEEEEAAPGAEGGVFPGPRGGAPGGGARLSPWLQPSALLFSLLCAFFWMGLYLLRAGVRLPLAVALLAACCGGEALVQIGLGVGEDHLLSLPAAGVVLSCLAAATWLVLRLRLGVLMIALTSAVRTVSLISLERFKVAWRPYLAYLAGVLGILLARYVEQILPQSAEAAPREHLGSQLIAGTKEDIPVFKRRRRSSSVVSAEMSGCSSKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLGSHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQSHSSEQIQAIKEEEEEKGKPRGEEIPTQKPDQ Hydrolyzes both cyclic AMP (cAMP) and cyclic GMP (cGMP) mint homomint arfgef1-arfgef2-1 3186342 N/A 3186353 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186354 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false pde3a-arfgef1-1 3186343 N/A 3186355 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186356 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3186353 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186354 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false arfgef2-pde3a-1 3186344 N/A 3186356 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186353 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false arfgef1-arfgef2-4 3186345 N/A 3186355 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186356 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186353 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186354 unspecified role unspecified role bait bait human Homo sapiens Human association association false false false arfgef2-arfgef1-1 3186346 N/A 3186356 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186353 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3186354 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false arfgef2-arfgef1-2 3186347 N/A 3186353 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3186354 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false arfgef1-arfgef2-5 3186348 N/A 3186356 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186353 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186354 unspecified role unspecified role bait bait human Homo sapiens Human association association false false false arfgef2-arfgef1-3 3186349 N/A 3186355 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186356 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186353 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3186354 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false pde3a-arfgef1-2 3186350 N/A 3186356 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3186353 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186354 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false arfgef1-arfgef2-6 3186351 N/A 3186355 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3186356 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3186353 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3186354 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human physical association physical association false false false arfgef1-pde3a-1 3186352 N/A 3186356 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3186354 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false