DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ estve-2009-1 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. DIP original experiment: DIP-134022X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells. Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. Only protein-protein interactions imex curation estve-2009-2 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. DIP original experiment: DIP-134029X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells. Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. Only protein-protein interactions imex curation estve-2009-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. DIP original experiment: DIP-134025X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells. Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. Only protein-protein interactions imex curation estve-2009-4 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. DIP original experiment: DIP-134027X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells. Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. Only protein-protein interactions imex curation estve-2009-5 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. DIP original experiment: DIP-134023X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells. Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. Only protein-protein interactions imex curation estve-2009-6 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. DIP original experiment: DIP-134028X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells. Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. Only protein-protein interactions imex curation estve-2009-7 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. DIP original experiment: DIP-134024X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells. Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. Only protein-protein interactions imex curation estve-2009-8 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. DIP original experiment: DIP-134026X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells. Estève PO., Chin HG., Benner J., Feehery GR., Samaranayake M., Horwitz GA., Jacobsen SE., Pradhan S. Only protein-protein interactions imex curation dnmt1_human DNA (cytosine-5)-methyltransferase 1 CXXC9 MCMT DNA methyltransferase HsaI CXXC-type zinc finger protein 9 DNMT1 AIM DNMT protein protein human Homo sapiens Human MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG setd7_human Histone-lysine N-methyltransferase SETD7 SETD7 KIAA1717 SET7 KMT7 SET9 Histone H3-K4 methyltransferase SETD7 SET domain-containing protein 7 SET7/9 Lysine N-methyltransferase 7 protein protein human Homo sapiens Human MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK h3-10 Histone 3 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) CARTKQTARKSTGG dnmt1-ub-1 3185765 N/A 3185773 unspecified role unspecified role bait bait chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3185774 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false dnmt1-setd7-1 3185766 N/A 3185773 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 136-147 Region 136-147 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3185775 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm methylation methylation reaction methylation false false false dnmt1-setd7-2 3185767 N/A 3185773 unspecified role unspecified role bait bait N-terminal domain Region 1-446 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false C-terminal domain Region 1081-1408 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3185775 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false setd7-dnmt1-1 3185768 N/A 3185773 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3185775 unspecified role unspecified role bait bait N-terminal domain Region 1-240 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false dnmt1-setd7-3 3185769 N/A 3185775 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3185773 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false h3-setd7-1 3185770 N/A 3185776 enzyme target enzyme target substrate substrate unspecified role unspecified role in vitro In vitro N/A 3185775 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm methylation methylation reaction methylation false false false dnmt1-setd7-4 3185771 N/A 3185773 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 92-159 Region 92-159 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3185775 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm methylation methylation reaction methylation false false false setd7-dnmt1-2 3185772 N/A 3185775 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3185773 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false