DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hatakeyama-2009-1 in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133800X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions imex curation hatakeyama-2009-2 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133796X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions imex curation hatakeyama-2009-3 in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133802X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions imex curation hatakeyama-2009-4 in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay tag visualisation tag visualisation Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133798X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions imex curation hatakeyama-2009-5 in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133799X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions imex curation hatakeyama-2009-6 in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133801X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions imex curation hatakeyama-2009-7 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133797X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions imex curation srsf5_mouse Serine/arginine-rich splicing factor 5 Srsf5 Hrs Sfrs5 Delayed-early protein HRS Pre-mRNA-splicing factor SRP40 Splicing factor, arginine/serine-rich 5 protein protein mouse Mus musculus Mouse MSGCRVFIGRLNPAAREKDVERFFKGYGRIRDIDLKRGFGFVEFEDPRDADDAVYELDGKELCSERVTIEHARARSRGGRGRGRYSDRFSSRRPRNDRRNAPPVRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPKLNEGVVEFASYGDLKNAIEKLSGKEINGRKIKLIEGSKRHRSRSRSRSRTRSSSRSRSRSRSRRSKSYSRSRSRSRSRSKSRSGSRSPVPEKSQKRGSSSRSKSPASVDRQRSRSRSRSRSVDSGN ipeptide I-peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) IELLQARC srsf7_mouse Serine/arginine-rich splicing factor 7 Srsf7 Sfrs7 Splicing factor, arginine/serine-rich 7 protein protein mouse Mus musculus Mouse MRSSARGRPLQAATAFFLSLFFFLRRFERGFWLWGGDSETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPRRSRFDRPPARRPFDPNDRCYECGEKGHYAYDCHRYSRRRRSRSRSRSHSRSRGRRYSRSRSRSRGRRSRSASPRRSRSVSLRRSRSASLRRSRSGSIIGSRYFQSRSRSRSRSRSISRPRSSRSKSRSPSPKRSRSPSGSPHRSASPERMD srsf2_mouse Serine/arginine-rich splicing factor 2 Sfrs2 Splicing factor, arginine/serine-rich 2 Srsf2 Pr264 Sfrs10 Splicing factor SC35 Splicing component, 35 kDa Protein PR264 Putative myelin regulatory factor 1 protein protein mouse Mus musculus Mouse MSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRSPRRRRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPVSKRESKSRSRSKSPPKSPEEEGAVSS srsf1_mouse Serine/arginine-rich splicing factor 1 Sfrs1 Splicing factor, arginine/serine-rich 1 Srsf1 ASF/SF2 Pre-mRNA-splicing factor SRp30a protein protein mouse Mus musculus Mouse MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSRT anxa1_human Annexin A1 ANXA1 ANX1 LPC1 Annexin-1 Annexin I Lipocortin I Calpactin II Chromobindin-9 p35 Phospholipase A2 inhibitory protein Calpactin-2 protein protein human Homo sapiens Human MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN srsf5-ipeptide-1 3184211 N/A 3184218 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3184219 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false ipeptide-srsf1-1 3184212 N/A 3184220 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3184218 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3184221 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3184222 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3184219 unspecified role unspecified role bait bait in vitro In vitro association association false false false anxa1-ipeptide-1 3184213 N/A 3184223 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3184219 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false srsf1-ipeptide-1 3184214 N/A 3184219 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3184222 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false srsf2-ipeptide-1 3184215 N/A 3184221 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3184219 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false srsf7-ipeptide-1 3184216 N/A 3184220 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3184219 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false ipeptide-srsf1-2 3184217 N/A 3184219 unspecified role unspecified role unspecified role unspecified role A488-label A488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3184222 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false