DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chang-2009-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. DIP original experiment: DIP-133778X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the eIF4A-PDCD4 complex. Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. Only protein-protein interactions imex curation chang-2009-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. DIP original experiment: DIP-133780X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the eIF4A-PDCD4 complex. Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. Only protein-protein interactions imex curation chang-2009-4 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. DIP original experiment: DIP-133779X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the eIF4A-PDCD4 complex. Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. Only protein-protein interactions imex curation chang-2009-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. DIP original experiment: DIP-133781X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the eIF4A-PDCD4 complex. Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. Only protein-protein interactions imex curation pdcd4_human Programmed cell death protein 4 PDCD4 H731 Nuclear antigen H731-like Neoplastic transformation inhibitor protein Protein 197/15a protein protein human Homo sapiens Human MDVENEQILNVNPADPDNLSDSLFSGDEENAGTEEIKNEINGNWISASSINEARINAKAKRRLRKNSSRDSGRGDSVSDSGSDALRSGLTVPTSPKGRLLDRRSRSGKGRGLPKKGGAGGKGVWGTPGQVYDVEEVDVKDPNYDDDQENCVYETVVLPLDERAFEKTLTPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYIDSYKGTVDCVQARAALDKATVLLSMSKGGKRKDSVWGSGGGQQSVNHLVKEIDMLLKEYLLSGDISEAEHCLKELEVPHFHHELVYEAIIMVLESTGESTFKMILDLLKSLWKSSTITVDQMKRGYERIYNEIPDINLDVPHSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY if4a1_human Eukaryotic initiation factor 4A-I EIF4A1 EIF4A DDX2A ATP-dependent RNA helicase eIF4A-1 protein protein human Homo sapiens Human MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI eif4a1-pdcd4-6 3184003 N/A 3184007 unspecified role unspecified role unspecified role unspecified role Region 164-469 Region 164-469 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q53EL6:p.[Glu249Ala;Asp253Ala;Asp414Ala;Asp418Ala] E24A/E253A/D414A/D418A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3184008 unspecified role unspecified role unspecified role unspecified role Region 20-406 Region 20-406 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false eif4a1-pdcd4-7 3184004 N/A 3184007 unspecified role unspecified role unspecified role unspecified role Region 164-469 Region 164-469 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3184008 unspecified role unspecified role unspecified role unspecified role Region 20-406 Region 20-406 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false eif4a1-pdcd4-8 3184005 N/A 3184007 unspecified role unspecified role unspecified role unspecified role Region 164-469 Region 164-469 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3184008 unspecified role unspecified role unspecified role unspecified role Region 20-406 Region 20-406 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false eif4a1-pdcd4-9 3184006 N/A 3184007 unspecified role unspecified role unspecified role unspecified role Region 164-469 Region 164-469 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3184008 unspecified role unspecified role unspecified role unspecified role Region 20-406 Region 20-406 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false