DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ su-2009-2 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216039X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions imex curation su-2009-3 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216035X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions imex curation su-2009-4 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216041X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions imex curation su-2009-5 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216042X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions imex curation su-2009-6 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216037X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions imex curation su-2009-7 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216038X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions imex curation su-2009-8 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216040X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions imex curation su-2009-9 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216036X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions imex curation 5p4 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) TTDRWYDDYHPEE pex14_rat Peroxisomal membrane protein PEX14 Pex14 PTS1 receptor-docking protein Peroxin-14 Peroxisomal membrane anchor protein PEX14 protein protein rat Rattus norvegicus (Rat) Rat MASSEQAEQPNQPSSSPGSENVVPREPLIATAVKFLQNSRVRQSPLATRRAFLKKKGLTDEEIDLAFQQSGTASDEPSPVGPATPVVPVQPPHLISQPYSPGGSRWRDYGALAIILAGIAFGFHQLYKKYLLPLILGGREDRKQLERMAASLSELSGSVAQTVTQVQTTLASVQELLRQQQQKVQELAHELAAAKATTSTNWILESQNINELKSEINSLKGLLLNRRQFPPSPSAPKIPSWQIPVKSPSPSSPAAVNHHSSSDISPVSNESPSSSPGKDGHSPEGSTATYRLLGPQEEGEGVVDVKGQVRMEVQGEEEKREDEEGEEDEDDDVSHVDEEDVLGVQREDRRGGDGQINEQVEKLRRPEGASNESERD This sequence has been withdrawn from Uniprot. C9DFFC0DD4DD45C8 pex5r_human PEX5-related protein PEX5L PEX5R PXR2 PEX2-related protein PEX5-like protein Peroxin-5-related protein Peroxisome biogenesis factor 5-like Tetratricopeptide repeat-containing Rab8b-interacting protein protein protein human Homo sapiens Human MYQGHMQKSKEQGYGKLSSDEDLEIIVDQKQGKGSRAADKAVAMVMKEIPREESAEEKPLLTMTSQLVNEQQESRPLLSPSIDDFLCETKSEAIARPVTSNTAVLTTGLDLLDLSEPVSQTQTKAKKSEPSSKTSSLKKKADGSDLISTDAEQRGQPLRVPETSSLDLDIQTQLEKWDDVKFHGDRNTKGHPMAERKSSSSRTGSKELLWSSEHRSQPELSGGKSALNSESASELELVAPTQARLTKEHRWGSALLSRNHSLEEEFERAKAAVESDTEFWDKMQAEWEEMARRNWISENQEAQNQVTISASEKGYYFHTENPFKDWPGAFEEGLKRLKEGDLPVTILFMEAAILQDPGDAEAWQFLGITQAENENEQAAIVALQRCLELQPNNLKALMALAVSYTNTGHQQDACDALKNWIKQNPKYKYLVKSKKGSPGLTRRMSKSPVDSSVLEGVKELYLEAAHQNGDMIDPDLQTGLGVLFHLSGEFNRAIDAFNAALTVRPEDYSLWNRLGATLANGDRSEEAVEAYTRALEIQPGFIRSRYNLGISCINLGAYREAVSNFLTALSLQRKSRNQQQVPHPAISGNIWAALRIALSLMDQPELFQAANLGDLDVLLRAFNLDP 5p6 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) TSEAWVDQFTRSG 5p7 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EAHPWLSDYDDLT 5p2 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) NETDWSQEFIAEV 5p3 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SPARWAEEYLEQS 5p5 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) QAEQWAAEFIQQQ 5p1 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) LSENWAQEFLAAG 5p4-pex14p-1 3182219 N/A 3182227 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3182228 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pex14p-pex5l-1 3182220 N/A 3182229 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3182228 unspecified role unspecified role bait bait Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q642G4:p.Phe35Ala F35A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q642G4:p.Phe52Ala F52A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q642G4:p.[Phe35Ala;Phe52Ala] F35A/F52A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 5p6-pex14p-1 3182221 N/A 3182230 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3182228 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 5p7-pex14p-1 3182222 N/A 3182231 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3182228 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 5p2-pex14p-1 3182223 N/A 3182232 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3182228 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 5p3-pex14p-1 3182224 N/A 3182233 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3182228 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 5p5-pex14p-1 3182225 N/A 3182234 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3182228 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false 5p1-pex14p-1 3182226 N/A 3182235 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3182228 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false