DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ suzuki-2009-8 canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. DIP original experiment: DIP-133607X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. Only protein-protein interactions imex curation suzuki-2009-9 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. DIP original experiment: DIP-133610X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. Only protein-protein interactions imex curation suzuki-2009-10 canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. DIP original experiment: DIP-133608X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. Only protein-protein interactions imex curation suzuki-2009-11 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. DIP original experiment: DIP-133609X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. Only protein-protein interactions imex curation ocln_canlf Occludin OCLN protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MSSRPFESPPPYRPDEFKPNHYAPSNDVYGGDMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLVIVMCIAIFGCVASTLAWDRGYGTGLMGGSIGYPYGSGFGSYGTGYGYGFGYGYGYGGYTDPRAAKGFLLAMVAFCFIAALVIFVTSVIRSDISRTRRYYLTVIILSAFLGVMMFIATIVYIMGVNPTAQASGSLYSSQIYAMCNQFYASTATGLYMDQYLYHYCVVDPQEAIAIVLGFMVIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDMPPPPSDYVERVDSPMAYSSNGKVNDKRLYPESSYKSTPVPEVVQELPATSPADDFRQPRYSSSGHLEPPSKRAPSKGRTGRPKRLEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQAELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSPDYKNKRNYCKQLKSKLSHIKKMVGDYDRQKT kpcl_human Protein kinase C eta type PRKCH PKCL PRKCL PKC-L nPKC-eta protein protein human Homo sapiens Human MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTKQKTNKPTYNEEFCANVTDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQINGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLIVTACTCQNNINKVDSKIAEQRFGINIPHKFSIHNYKVPTFCDHCGSLLWGIMRQGLQCKICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLVSRSTLRRQGKESSKEGNGIGVNSSNRLGIDNFEFIRVLGKGSFGKVMLARVKETGDLYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHDKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHAILRHPFFKEIDWAQLNHRQIEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP This sequence has been withdrawn from Uniprot. The sequence has been withdrawn from uniprot. q16625-1 Occludin OCLN WT-OCLN TM4(+) protein protein human Homo sapiens Human MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT pkceta-ocln-1 3294039 N/A 3294043 unspecified role unspecified role prey prey canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris N/A 3294044 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris physical association physical association false false false ocln-pkceta-1 3294040 N/A 3294044 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3294045 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 378-522 Region 378-522 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q16625-1:p.Thr403Ala T403A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q16625-1:p.Thr404Ala T404A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q16625-1:p.Thr438Ala T438A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false pT403 pT403 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false pT404 pT404 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false pS408 pS408 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false ocln-pkceta-2 3294041 N/A 3294043 unspecified role unspecified role bait bait canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris N/A 3294044 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris physical association physical association false false false ocln-pkceta-3 3294042 N/A 3294044 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3294045 unspecified role unspecified role bait bait Region 378-522 Region 378-522 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false