DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jovanovic_talisman-2009-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249953X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation jovanovic_talisman-2009-2 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249956X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation jovanovic_talisman-2009-3 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting tag fluorescence tag visualisation by fluorescence tag fluorescence Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249959X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation jovanovic_talisman-2009-4 ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249954X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation jovanovic_talisman-2009-5 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249961X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation jovanovic_talisman-2009-6 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel tag fluorescence tag visualisation by fluorescence tag fluorescence Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249957X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation jovanovic_talisman-2009-7 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249955X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation jovanovic_talisman-2009-8 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249960X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation jovanovic_talisman-2009-9 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249962X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation jovanovic_talisman-2009-10 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting tag fluorescence tag visualisation by fluorescence tag fluorescence Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249958X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions imex curation ntf2_human Nuclear transport factor 2 Placental protein 15 NUTF2 NTF2 protein protein human Homo sapiens Human MGDKPIWEQIGSSFIQHYYQLFDNDRTQLGAIYIDASCLTWEGQQFQGKAAIVEKLSSLPFQKIQHSITAQDHQPTPDSCIISMVVGQLKADEDPIMGFHQMFLLKNINDAWVCTNDMFRLALHNFG ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL imb1_yeast Importin subunit beta-1 KAP95 YLR347C L8300.15 Karyopherin subunit beta-1 Karyopherin-95 Importin-95 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTAEFAQLLENSILSPDQNIRLTSETQLKKLSNDNFLQFAGLSSQVLIDENTKLEGRILAALTLKNELVSKDSVKTQQFAQRWITQVSPEAKNQIKTNALTALVSIEPRIANAAAQLIAAIADIELPHGAWPELMKIMVDNTGAEQPENVKRASLLALGYMCESADPQSQALVSSSNNILIAIVQGAQSTETSKAVRLAALNALADSLIFIKNNMEREGERNYLMQVVCEATQAEDIEVQAAAFGCLCKIMSLYYTFMKPYMEQALYALTIATMKSPNDKVASMTVEFWSTICEEEIDIAYELAQFPQSPLQSYNFALSSIKDVVPNLLNLLTRQNEDPEDDDWNVSMSAGACLQLFAQNCGNHILEPVLEFVEQNITADNWRNREAAVMAFGSIMDGPDKVQRTYYVHQALPSILNLMNDQSLQVKETTAWCIGRIADSVAESIDPQQHLPGVVQACLIGLQDHPKVATNCSWTIINLVEQLAEATPSPIYNFYPALVDGLIGAANRIDNEFNARASAFSALTTMVEYATDTVAETSASISTFVMDKLGQTMSVDENQLTLEDAQSLQELQSNILTVLAAVIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIEDDVFYAISALAASLGKGFEKYLETFSPYLLKALNQVDSPVSITAVGFIADISNSLEEDFRRYSDAMMNVLAQMISNPNARRELKPAVLSVFGDIASNIGADFIPYLNDIMALCVAAQNTKPENGTLEALDYQIKVLEAVLDAYVGIVAGLHDKPEALFPYVGTIFQFIAQVAEDPQLYSEDATSRAAVGLIGDIAAMFPDGSIKQFYGQDWVIDYIKRTRSGQLFSQATKDTARWAREQQKRQLSL ED530F5313E75B06 nsp1_yeast Nucleoporin NSP1 NSP1 YJL041W J1207 Nuclear pore protein NSP1 Nucleoskeletal-like protein p110 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNFNTPQQNKTPFSFGTANNNSNTTNQNSSTGAGAFGTGQSTFGFNNSAPNNTNNANSSITPAFGSNNTGNTAFGNSNPTSNVFGSNNSTTNTFGSNSAGTSLFGSSSAQQTKSNGTAGGNTFGSSSLFNNSTNSNTTKPAFGGLNFGGGNNTTPSSTGNANTSNNLFGATANANKPAFSFGATTNDDKKTEPDKPAFSFNSSVGNKTDAQAPTTGFSFGSQLGGNKTVNEAAKPSLSFGSGSAGANPAGASQPEPTTNEPAKPALSFGTATSDNKTTNTTPSFSFGAKSDENKAGATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKPDENKASATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKSDEKKDSDSSKPAFSFGTKSNEKKDSGSSKPAFSFGAKPDEKKNDEVSKPAFSFGAKANEKKESDESKSAFSFGSKPTGKEEGDGAKAAISFGAKPEEQKSSDTSKPAFTFGAQKDNEKKTEESSTGKSTADVKSSDSLKLNSKPVELKPVSLDNKTLDDLVTKWTNQLTESASHFEQYTKKINSWDQVLVKGGEQISQLYSDAVMAEHSQNKIDQSLQYIERQQDELENFLDNFETKTEALLSDVVSTSSGAAANNNDQKRQQAYKTAQTLDENLNSLSSNLSSLIVEINNVSNTFNKTTNIDINNEDENIQLIKILNSHFDALRSLDDNSTSLEKQINSIKK A258003CB1917810 nu100_yeast Nucleoporin NUP100/NSP100 NUP100 NSP100 YKL068W YKL336 Nuclear pore protein NUP100/NSP100 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFGNNRPMFGGSNLSFGSNTSSFGGQQSQQPNSLFGNSNNNNNSTSNNAQSGFGGFTSAAGSNSNSLFGNNNTQNNGAFGQSMGATQNSPFGSLNSSNASNGNTFGGSSSMGSFGGNTNNAFNNNSNSTNSPFGFNKPNTGGTLFGSQNNNSAGTSSLFGGQSTSTTGTFGNTGSSFGTGLNGNGSNIFGAGNNSQSNTTGSLFGNQQSSAFGTNNQQGSLFGQQSQNTNNAFGNQNQLGGSSFGSKPVGSGSLFGQSNNTLGNTTNNRNGLFGQMNSSNQGSSNSGLFGQNSMNSSTQGVFGQNNNQMQINGNNNNSLFGKANTFSNSASGGLFGQNNQQQGSGLFGQNSQTSGSSGLFGQNNQKQPNTFTQSNTGIGLFGQNNNQQQQSTGLFGAKPAGTTGSLFGGNSSTQPNSLFGTTNVPTSNTQSQQGNSLFGATKLTNMPFGGNPTANQSGSGNSLFGTKPASTTGSLFGNNTASTTVPSTNGLFGNNANNSTSTTNTGLFGAKPDSQSKPALGGGLFGNSNSNSSTIGQNKPVFGGTTQNTGLFGATGTNSSAVGSTGKLFGQNNNTLNVGTQNVPPVNNTTQNALLGTTAVPSLQQAPVTNEQLFSKISIPNSITNPVKATTSKVNADMKRNSSLTSAYRLAPKPLFAPSSNGDAKFQKWGKTLERSDRGSSTSNSITDPESSYLNSNDLLFDPDRRYLKHLVIKNNKNLNVINHNDDEASKVKLVTFTTESASKDDQASSSIAASKLTEKAHSPQTDLKDDHDESTPDPQSKSPNGSTSIPMIENEKISSKVPGLLSNDVTFFKNNYYISPSIETLGNKSLIELRKINNLVIGHRNYGKVEFLEPVDLLNTPLDTLCGDLVTFGPKSCSIYENCSIKPEKGEGINVRCRVTLYSCFPIDKETRKPIKNITHPLLKRSIAKLKENPVYKFESYDPVTGTYSYTIDHPVLT D3985F9901BBAA51 ima1_yeast Importin subunit alpha SRP1 KAP60 YNL189W N1606 Karyopherin subunit alpha Karyopherin-60 Serine-rich RNA polymerase I suppressor protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNGTDSSTSKFVPEYRRTNFKNKGRFSADELRRRRDTQQVELRKAKRDEALAKRRNFIPPTDGADSDEEDESSVSADQQFYSQLQQELPQMTQQLNSDDMQEQLSATVKFRQILSREHRPPIDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLLEVAEYKTKKEACWAISNASSGGLQRPDIIRYLVSQGCIKPLCDLLEIADNRIIEVTLDALENILKMGEADKEARGLNINENADFIEKAGGMEKIFNCQQNENDKIYEKAYKIIETYFGEEEDAVDETMAPQNAGNTFGFGSNVNQQFNFN 8D3A0CB76F2E7C00 nutf2-1 3181873 N/A 3181883 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false nutf2-rangdp-1 3181874 N/A 3181884 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3181883 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false nsp1fg-kap95-1 3181875 N/A 3181885 unspecified role unspecified role prey prey Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3181886 unspecified role unspecified role bait bait Region 30-591 Region 30-591 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nutf2-2 3181876 N/A 3181883 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false nup100fg-kap95-1 3181877 N/A 3181887 unspecified role unspecified role bait bait Region 1-570 Region 1-570 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3181885 unspecified role unspecified role prey prey Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false ibb-kap95-1 3181878 N/A 3181888 unspecified role unspecified role unspecified role unspecified role Region 1-65 Region 1-65 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false eGFP-tag eGFP-tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3181885 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ibb-kap95-2 3181879 N/A 3181888 unspecified role unspecified role unspecified role unspecified role Region 1-65 Region 1-65 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3181885 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false nup100fg-nutf2-1 3181880 N/A 3181887 unspecified role unspecified role bait bait Region 1-570 Region 1-570 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3181883 unspecified role unspecified role prey prey YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false nup100fg-kap95-2 3181881 N/A 3181887 unspecified role unspecified role bait bait Region 1-570 Region 1-570 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3181885 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false nsp1fg-nutf2-1 3181882 N/A 3181883 unspecified role unspecified role prey prey YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3181886 unspecified role unspecified role bait bait Region 30-591 Region 30-591 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false