DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chandra-2008-1 in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. DIP original experiment: DIP-249984X Nature 2008 IMEx Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. Only protein-protein interactions imex curation chandra-2008-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. DIP original experiment: DIP-249982X Nature 2008 IMEx Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. Only protein-protein interactions imex curation chandra-2008-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. DIP original experiment: DIP-249983X Nature 2008 IMEx Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. Only protein-protein interactions imex curation p37231-2 Peroxisome proliferator-activated receptor gamma PPARG NR1C3 Nuclear receptor subfamily 1 group C member 3 PPARgamma1(wt) protein protein human Homo sapiens Human MTMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY rxra_human Retinoic acid receptor RXR-alpha RXRA NR2B1 Retinoid X receptor alpha Nuclear receptor subfamily 2 group B member 1 protein protein human Homo sapiens Human MDTKHFLPLDFSTQVNSSLTSPTGRGSMAAPSLHPSLGPGIGSPGQLHSPISTLSSPINGMGPPFSVISSPMGPHSMSVPTTPTLGFSTGSPQLSSPMNPVSSSEDIKPPLGLNGVLKVPAHPSGNMASFTKHICAICGDRSSGKHYGVYSCEGCKGFFKRTVRKDLTYTCRDNKDCLIDKRQRNRCQYCRYQKCLAMGMKREAVQEERQRGKDRNENEVESTSSANEDMPVERILEAELAVEPKTETYVEANMGLNPSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSIAVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFNPDSKGLSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQMT coactivator peptide Coactivator peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EKHKILHRLLQDSY pparg-rxra-1 3180802 N/A 3180805 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3180806 unspecified role unspecified role unspecified role unspecified role in vitro In vitro physical association physical association false false false coactivator peptide-pparg-1 3180803 N/A 3180807 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3180805 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3180806 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false coactivator peptide-pparg-2 3180804 N/A 3180807 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3180805 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3180806 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false