DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ singer-2008-1 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Singer AU., Rohde JR., Lam R., Skarina T., Kagan O., Dileo R., Chirgadze NY., Cuff ME., Joachimiak A., Tyers M., Sansonetti PJ., Parsot C., Savchenko A. DIP original experiment: DIP-113511X Nature structural & molecular biology 2008 IMEx Structure of the Shigella T3SS effector IpaH defines a new class of E3 ubiquitin ligases. Singer AU., Rohde JR., Lam R., Skarina T., Kagan O., Dileo R., Chirgadze NY., Cuff ME., Joachimiak A., Tyers M., Sansonetti PJ., Parsot C., Savchenko A. Only protein-protein interactions imex curation singer-2008-2 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Singer AU., Rohde JR., Lam R., Skarina T., Kagan O., Dileo R., Chirgadze NY., Cuff ME., Joachimiak A., Tyers M., Sansonetti PJ., Parsot C., Savchenko A. DIP original experiment: DIP-113512X Nature structural & molecular biology 2008 IMEx Structure of the Shigella T3SS effector IpaH defines a new class of E3 ubiquitin ligases. Singer AU., Rohde JR., Lam R., Skarina T., Kagan O., Dileo R., Chirgadze NY., Cuff ME., Joachimiak A., Tyers M., Sansonetti PJ., Parsot C., Savchenko A. Only protein-protein interactions imex curation singer-2008-3 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Singer AU., Rohde JR., Lam R., Skarina T., Kagan O., Dileo R., Chirgadze NY., Cuff ME., Joachimiak A., Tyers M., Sansonetti PJ., Parsot C., Savchenko A. DIP original experiment: DIP-113514X Nature structural & molecular biology 2008 IMEx Structure of the Shigella T3SS effector IpaH defines a new class of E3 ubiquitin ligases. Singer AU., Rohde JR., Lam R., Skarina T., Kagan O., Dileo R., Chirgadze NY., Cuff ME., Joachimiak A., Tyers M., Sansonetti PJ., Parsot C., Savchenko A. Only protein-protein interactions imex curation singer-2008-4 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Singer AU., Rohde JR., Lam R., Skarina T., Kagan O., Dileo R., Chirgadze NY., Cuff ME., Joachimiak A., Tyers M., Sansonetti PJ., Parsot C., Savchenko A. DIP original experiment: DIP-113513X Nature structural & molecular biology 2008 IMEx Structure of the Shigella T3SS effector IpaH defines a new class of E3 ubiquitin ligases. Singer AU., Rohde JR., Lam R., Skarina T., Kagan O., Dileo R., Chirgadze NY., Cuff ME., Joachimiak A., Tyers M., Sansonetti PJ., Parsot C., Savchenko A. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG a0a0h2usg1_shifl RING-type E3 ubiquitin transferase ipaH1.4 SF_p0265 protein protein shifl Shigella flexneri MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKTWSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNLLTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHNNRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSATISAPEHSLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYSQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSNHIA ipa9_shifl E3 ubiquitin-protein ligase ipaH9.8 ipaH9.8 Invasion plasmid antigen ipaH9.8 RING-type E3 ubiquitin transferase ipaH9.8 SFLP090 pWR501_0234 CP0226 protein protein shifl Shigella flexneri MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSSHALPALQRLTSSPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS ub2d2_human Ubiquitin-conjugating enzyme E2 D2 UBC5B UBCH4 UBE2D2 UBC4 UBCH5B Ubiquitin-protein ligase D2 Ubiquitin carrier protein D2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-conjugating enzyme E2(17)KB 2 PUBC1 p53-regulated ubiquitin-conjugating enzyme 1 (E3-independent) E2 ubiquitin-conjugating enzyme D2 E2 ubiquitin-conjugating enzyme D2 protein protein human Homo sapiens Human MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM ipah1.4-ub-1 3294012 N/A 3294016 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3294017 unspecified role unspecified role unspecified role unspecified role Region 265-575 Region 265-575 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ipah9.8-ub-1 3294013 N/A 3294018 unspecified role unspecified role unspecified role unspecified role Region 208-545 Region 208-545 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3294016 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ipah1.4-ube2d2-1 3294014 N/A 3294017 unspecified role unspecified role unspecified role unspecified role Region 265-575 Region 265-575 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3294019 unspecified role unspecified role unspecified role unspecified role Fluorescent-label Fluorescent-label fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false ipah9.8-ube2d2-1 3294015 N/A 3294019 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3294018 unspecified role unspecified role bait bait Region 208-545 Region 208-545 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false