DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lai-2008-7 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Lai KO., Zhao Y., Ch'ng TH., Martin KC. DIP original experiment: DIP-133440X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Importin-mediated retrograde transport of CREB2 from distal processes to the nucleus in neurons. Lai KO., Zhao Y., Ch'ng TH., Martin KC. Only protein-protein interactions imex curation lai-2008-8 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Lai KO., Zhao Y., Ch'ng TH., Martin KC. DIP original experiment: DIP-133438X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Importin-mediated retrograde transport of CREB2 from distal processes to the nucleus in neurons. Lai KO., Zhao Y., Ch'ng TH., Martin KC. Only protein-protein interactions imex curation lai-2008-9 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Lai KO., Zhao Y., Ch'ng TH., Martin KC. DIP original experiment: DIP-133439X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Importin-mediated retrograde transport of CREB2 from distal processes to the nucleus in neurons. Lai KO., Zhao Y., Ch'ng TH., Martin KC. Only protein-protein interactions imex curation ima7_mouse Importin subunit alpha-7 Kpna6 Importin alpha-S2 Karyopherin subunit alpha-6 Kpna5 protein protein mouse Mus musculus Mouse METMASPGKDNYRMKSYKNNALNPEEMRRRREEEGIQLRKQKREQQLFKRRNVELINEEAAMFDSLLMDSYVSSTTGESVITREMVEMLFSDDSDLQLATTQKFRKLLSKEPSPPIDEVINTPGVVDRFVEFLKRNENCTLQFEAAWALTNIASGTSQQTKIVIEAGAVPIFIELLNSDFEDVQEQAVWALGNIAGDSSLCRDYVLNCSILNPLLTLLTKSTRLTMTRNAVWALSNLCRGKNPPPEFAKVSPCLPVLSRLLFSSDSDLLADACWALSYLSDGPNEKIQAVIDSGVCRRLVELLMHNDYKVASPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSSSKESIRKEACWTISNITAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRYLVSLGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQESKRSGSGVNPYCGLIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGVEDDDSSLAPQVDETQQQFIFQQPEAPMEGFQL atf4_mouse Cyclic AMP-dependent transcription factor ATF-4 Atf4 C/EBP-related ATF Activating transcription factor 4 Creb2 Cyclic AMP-responsive element-binding protein 2 protein protein mouse Mus musculus Mouse MTEMSFLNSEVLAGDLMSPFDQSGLGAEESLGLLDDYLEVAKHLKPHGFSSDKAGSSEWPAMDDGLASASDTGKEDAFSGTDWMLEKMDLKEFDFDALFRMDDLETMPDELLTTLDDTCDLFAPLVQETNKEPPQTVNPIGHLPESLIKVDQVAPFTFLQPFPCSPGVLSSTPEHSFSLELGSEVDISEGDRKPDSAAYITLIPPCVKEEDTPSDNDSGICMSPESYLGSPQHSPSTSRAPPDNLPSPGGSRGSPRPKPYDPPGVSLTAKVKTEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKEKADSLAKEIQYLKDLIEEVRKARGKKRVP ima5_mouse Importin subunit alpha-5 Kpna1 Rch2 Importin alpha-S1 Karyopherin subunit alpha-1 SRP1-beta RAG cohort protein 2 Nucleoprotein interactor 1 protein protein mouse Mus musculus Mouse MSTPGKENFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQLFKRRNVATAEEETEEEVMSDGGFHEAQINNMEMAPGGVITSDMTDMIFSNSPEQQLSATQKFRKLLSKEPNPPIDEVINTPGVVARFVEFLKRKENCTLQFESAWVLTNIASGNSLQTRNVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLNCNILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLNVLSWLLFVSDTDVLADACWALSYLSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSPKESIKKEACWTISNITAGNRAQIQTVIDANMFPALISILQTAEFRTRKEAAWAITNATSGGSAEQIKYLVELGCIKPLCDLLTVMDAKIVQVALNGLENILRLGEQEAKRNGSGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGTEDEDSSIAPQVDLSQQQYIFQQCEAPMEGFQL mint Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus q6qjf2_aplca Importin subunit alpha protein protein aplca Aplysia californica (California sea hare) California sea hare MATDPAGKGTRGLMFKNKGKDADEMRRRRTEVTVELRKNKKEEHMLKRRNVPTQDSTDSEDNEKPTTQSLETIVANASSPEPEVQLGAVQAARKLLSSDRNPPIDDLITSGILPILVNCLSRDDNPSLQFEAAWAITNIASGTSAQTQAVVNAGAVPFFLQLLRSPHQNVCEQAVWALGNIIGDGPECRNYVISLGAVQHLLTFINPNIPLPFLRNVAWVIVNLCRNKDPPPPAETIEEILPALCTLIHHSDVNILVDTVWALSYLTDGGNDQIQMVIDSGVVPFLVPLLCHQDVKLQTAALRAVGNIVTGTDEQTQAVLNCNALQHFSSLLTHPKEKINKEAVWFLSNITAGNQQQVQAVIDASLIPLIIHHLNRGDFQTQKEAAWAISNLTISGKKEQVAYVVEMGVLPPFCNLLSVKDAQVVNVVLDGINNILKMAGEDVDQICQIIEECGGLDKIENLQNHENEEIYKLSYEIIDSFFQDEDQEESLLPQASQEGYQFDPNAQIPQEGFKF q16946_aplca ApCREB2 protein protein aplca Aplysia californica (California sea hare) California sea hare MELDLWSEDFQLAREWGLEMPVVQTDGQFGDLKSTSRHGGDESLSLQPQGATLKLEPFEEDVLGAEWMESSDLGSFLDALGDNHERLHPFESNLLEFTSLITPDDSTVSKDILSSTLQFPTQPVNIPLYASHGAEDFSAETEFENHLSPPDSPEQVAPVINLEPVELTASHMTVISPDGLLGGMELASESLTFTELDFVNFNDSAVGSIGGAEELLGSPLSVDDVESTISFSGPSSPETSQSSIIESSPELYKVISTSSIDASKRFSPYSRSSKSKQSVKTSDAKAPRKTRTPAQPVPEHVIMEHLDKKDRKKLQNKNAAIRYRMKKKGEAQGIKGEEQELEELNTKLKTKVDDLQREIKYMKNLMEDVCKAKGIQLK atf4-kpna6-1 3179504 N/A 3179507 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3179508 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false atf4-kpna1-1 3179505 N/A 3179509 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3179508 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false apcreb2-q6qjf2_aplca-1 3179506 N/A 3179510 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3179511 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false