DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ han-2008-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133199X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-7 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133201X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-8 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133192X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-9 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133197X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-10 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133195X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-11 human Homo sapiens Human comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133203X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-12 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133193X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-13 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133198X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-14 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133200X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-15 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133194X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-16 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133196X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation han-2008-17 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. DIP original experiment: DIP-133202X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx AIMP2/p38, the scaffold for the multi-tRNA synthetase complex, responds to genotoxic stresses via p53. Han JM., Park BJ., Park SG., Oh YS., Choi SJ., Lee SW., Hwang SK., Chang SH., Cho MH., Kim S. Only protein-protein interactions imex curation aimp2_human Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 Protein JTV-1 AIMP2 PRO0992 JTV1 Multisynthase complex auxiliary component p38 protein protein human Homo sapiens Human MPMYQVKPYHGGGAPLRVELPTCMYRLPNVHGRSYGPAPGAGHVQEESNLSLQALESRQDDILKRLYELKAAVDGLSKMIQTPDADLDVTNIIQADEPTTLTTNALDLNSVLGKDYGALKDIVINANPASPPLSLLVLHRLLCEHFRVLSTVHTHSSVKSVPENLLKCFGEQNKKQPRQDYQLGFTLIWKNVPKTQMKFSIQTMCPIEGEGNIARFLFSLFGQKHNAVNATLIDSWVDIAIFQLKEGSSKEKAAVFRSMNSALGKSPWLAGNELTVADVVLWSVLQQIGGCSVTVPANVQRWMRSCENLAPFNTALKLLK p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD mdm2_mouse E3 ubiquitin-protein ligase Mdm2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein Mdm2 RING-type E3 ubiquitin transferase Mdm2 protein protein mouse Mus musculus Mouse MCNTNMSVSTEGAASTSQIPASEQETLVRPKPLLLKLLKSVGAQNDTYTMKEIIFYIGQYIMTKRLYDEKQQHIVYCSNDLLGDVFGVPSFSVKEHRKIYAMIYRNLVAVSQQDSGTSLSESRRQPEGGSDLKDPLQAPPEEKPSSSDLISRLSTSSRRRSISETEENTDELPGERHRKRRRSLSFDPSLGLCELREMCSGGSSSSSSSSSESTETPSHQDLDDGVSEHSGDCLDQDSVSDQFSVEFEVESLDSEDYSLSDEGHELSDEDDEVYRVTVYQTGESDTDSFEGDPEISLADYWKCTSCNEMNPPLPSHCKRCWTLRENWLPDDKGKDKVEISEKAKLENSAQAEEGLDVPDGKKLTENDAKEPCAEEDSEEKAEQTPLSQESDDYSQPSTSSSIVYSSQESVKELKEETQDKDESVESSFSLNAIEPCVICQGRPKNGCIVHGKTGHLMSCFTCAKKLKKRNKPCPVCRQPIQMIVLTYFN syep_human Bifunctional glutamate/proline--tRNA ligase EPRS1 Bifunctional aminoacyl-tRNA synthetase Cell proliferation-inducing gene 32 protein EPRS GLNS Glutamatyl-prolyl-tRNA synthetase PARS QARS QPRS PIG32 protein protein human Homo sapiens Human MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG tp53-aimp2-1 3175787 N/A 3175799 unspecified role unspecified role prey prey His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3175800 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false tp53-mdm2-30 3175788 N/A 3175801 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3175802 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false aimp2-eprs-1 3175789 N/A 3175803 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3175799 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human physical association physical association false false false aimp2-tp53-1 3175790 N/A 3175800 unspecified role unspecified role prey prey P04637:p.Leu22_Trp23delinsGlnSer mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3175799 unspecified role unspecified role bait bait Gst tag Gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false tp53-aimp2-2 3175791 N/A 3175799 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3175800 unspecified role unspecified role bait bait binding site 261-393 binding site 261-393 required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false tp53-ub-3 3175792 N/A 3175804 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3175800 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human covalent binding covalent binding false false false tp53-aimp2-3 3175793 N/A 3175799 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3175800 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false tp53-aimp2-4 3175794 N/A 3175800 unspecified role unspecified role bait bait Gst tag Gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3175799 unspecified role unspecified role prey prey binding site 162-225 binding site 162-225 required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false ub-aimp2-1 3175795 N/A 3175799 unspecified role unspecified role prey prey Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3175800 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3175804 unspecified role unspecified role bait bait ha tag ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human association association false false false tp53-aimp2-5 3175796 N/A 3175799 unspecified role unspecified role prey prey His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3175800 unspecified role unspecified role bait bait Gst tag Gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false tp53-ub-4 3175797 N/A 3175804 unspecified role unspecified role prey prey ha tag ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false mouse Mus musculus Mouse N/A 3175800 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false tp53-ub-5 3175798 N/A 3175804 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3175800 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false