DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ he-2008-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. DIP original experiment: DIP-250331X Nature 2008 IMEx Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. Only protein-protein interactions imex curation he-2008-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. DIP original experiment: DIP-250329X Nature 2008 IMEx Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. Only protein-protein interactions imex curation he-2008-3 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. DIP original experiment: DIP-250330X Nature 2008 IMEx Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. Only protein-protein interactions imex curation rdrp_i96a0 RNA-directed RNA polymerase catalytic subunit PB1 Polymerase basic protein 1 RNA-directed RNA polymerase subunit P1 protein protein i96a0 Influenza A virus (strain A/Goose/Guangdong/1/1996 H5N1 genotype Gs/Gd) MDVNPTLLFLKVPAQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHQYSEKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHPGIFENSCLETMEVVQQTRVDKLTQGRQTYDWTLKRNQPAATALANTIEVFRSNGLTANESGRLIDFLKDVMESMDKGEMEIITHFQRKRRVRDNMTKKMVTQRTIGKKKQRLNKRSYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRGFVYFVETLARSICEKLEQSGLPVGGNEKKAKLANVVRKMMTNSQDTELSFTITGDNTKWNENQNPRMFLAMITYITRNQPEWFRNVLSIAPIMFSNKMARLGKGYMFESKSMKLRTQIPAEMLASIDLKYFNESTRKKIEKIRPLLIDGTASLSPGMMMGMFNMLSTVLGVSILNLGQKRYTKTTYWWDGLQSSDDFALIVNAPNHEGIEAGVDRFYRTCKLVGINMTKKKSYINRTGTCEFTSFFYRYGFVANFSMELPSFGVSGINESADMSIGVTVIKNNMMDNDLGPATAQMALQLFIKDYRYPYRCHRGDTQIQTRRSFELKKLWEQTRSKAGLLVSDGGPNPYNIRNLHIPEAGLKWELMDEDYQGRLCNPLNPFVSHKEIESVNNAVVMPAHGPAKSMEYDAVATTHSWIPKRNRSILNTSQRGILEDEQMYQKCCNLFEKFFPSSSYRRPVGISSMVEAMVSRARIDARIDFESGRIKKEEFAEIMKICSTIEELGRQK mint homomint pa_i96a0 Polymerase acidic protein PA RNA-directed RNA polymerase subunit P2 protein protein i96a0 Influenza A virus (strain A/Goose/Guangdong/1/1996 H5N1 genotype Gs/Gd) MEDFVRQCFNPMIVELAEKAMKEYGEDPKIETNKFAAICTHLEVCFMYSDFHFIDERGESTIIESGDPNALLKHRFEIIEGRDRTMAWTVVNSICNTTGVEKPKFLPDLYDYKENRFIEIGVTRREVHTYYLEKANKIKSEKTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMASRGLWDSFRQSERGEETVEERFEITGTMCRLADQSLPPNFSSLEKFRAYVDGFEPNGCIEGKLSQMSKEVNARIEPFLKTTPRPLRLPDGPPCSQRSKFLLMDALKLSIEDPSHEGEGIPLYDAIKCMKTFFGWKEPNIVKPHEKGINPNYLLAWKQVLAELQDIENEEKIPKTKNMRKTSQLKWALGENMAPEKVDFEDCKDVSDLRQYDSDEPKPRSLASWIQSEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEVSHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNLYGFLIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHRWEKYCVLRIGDMLLRTEIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCPFQSLQQIESMIEAESSVKEKDMTKEFFENKSETWPIGESPKGVEEGSIGKVCRTLLAKSVFNSLYASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLNASWFNSFLTHALR pb1-pa-4 3174450 N/A 3174453 unspecified role unspecified role bait bait Region 1-25 Region 1-25 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3174454 unspecified role unspecified role prey prey Region 257-716 Region 257-716 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9Q0U9:p.[Gln670Ala;Trp706Ala] W706/Q670A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9Q0U9:p.[Leu666Gly;Phe710Glu] L666G/F710E/G mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9Q0U9:p.[Trp706Ala;Phe710Gln] W706/F710Q mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9Q0U9:p.[Leu666Gly;Phe710Gly] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pa-pb1-1 3174451 N/A 3174453 unspecified role unspecified role unspecified role unspecified role Region 1-25 Region 1-25 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3174454 unspecified role unspecified role unspecified role unspecified role Region 257-716 Region 257-716 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pa-pb1-2 3174452 N/A 3174453 unspecified role unspecified role unspecified role unspecified role Region 1-25 Region 1-25 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3174454 unspecified role unspecified role unspecified role unspecified role Region 257-716 Region 257-716 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false