DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lu-2008-7 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Lu X., Zhang Y., Shin YK. DIP original experiment: DIP-113611X Nature structural & molecular biology 2008 IMEx Supramolecular SNARE assembly precedes hemifusion in SNARE-mediated membrane fusion. Lu X., Zhang Y., Shin YK. Only protein-protein interactions imex curation lu-2008-8 ecolx Escherichia coli epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Lu X., Zhang Y., Shin YK. DIP original experiment: DIP-113612X Nature structural & molecular biology 2008 IMEx Supramolecular SNARE assembly precedes hemifusion in SNARE-mediated membrane fusion. Lu X., Zhang Y., Shin YK. Only protein-protein interactions imex curation sso1_yeast Protein SSO1 SSO1 P1405 YPL232W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSYNNPYQLETPFEESYELDEGSSAIGAEGHDFVGFMNKISQINRDLDKYDHTINQVDSLHKRLLTEVNEEQASHLRHSLDNFVAQATDLQFKLKNEIKSAQRDGIHDTNKQAQAENSRQRFLKLIQDYRIVDSNYKEENKEQAKRQYMIIQPEATEDEVEAAISDVGGQQIFSQALLNANRRGEAKTALAEVQARHQELLKLEKSMAELTQLFNDMEELVIEQQENVDVIDKNVEDAQLDVEQGVGHTDKAVKSARKARKNKIRCWLIVFAIIVVVVVVVVVPAVVKTR 6E4C6D35C3CD9D94 snc2_yeast Synaptobrevin homolog 2 SNC2 YOR327C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSVPYDPYVPPEESNSGANPNSQNKTAALRQEIDDTVGIMRDNINKVAERGERLTSIEDKADNLAISAQGFKRGANRVRKQMWWKDLKMRMCLFLVVIILLVVIIVPIVVHFS 815F027FAD8A599B sso1p-snc2p-1 3173227 N/A 3173229 unspecified role unspecified role fluorescence donor fluorescence donor Region 185-290 Region 185-290 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Cy3-label Cy3-label cy3 label cy3 label certain certain sequence position certain certain certain sequence position certain false P32867:p.Glu185Cys E185C mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3173230 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Cy5-label Cy5-label cy5 label cy5 label certain certain sequence position certain certain certain sequence position certain false P33328:p.Pro13Cys P13C mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sso1p-1 3173228 N/A 3173229 unspecified role unspecified role unspecified role unspecified role MTSSL-label MTSSL-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false MTSSL-label MTSSL-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false MTSSL-label MTSSL-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false MTSSL-label MTSSL-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false Region 185-290 Region 185-290 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false