DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ douglas-2008-1 in vitro In vitro peptide array peptide array western blot western blot Immuno blot Immuno blot Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. DIP original experiment: DIP-111013X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. Only protein-protein interactions imex curation douglas-2008-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. DIP original experiment: DIP-111010X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. Only protein-protein interactions imex curation douglas-2008-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast comigration in gel comigration in gel electrophoresis comigration in gel anti tag western anti tag western blot anti tag western Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. DIP original experiment: DIP-111007X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. Only protein-protein interactions imex curation douglas-2008-4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. DIP original experiment: DIP-111011X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. Only protein-protein interactions imex curation douglas-2008-5 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast comigration in gel comigration in gel electrophoresis comigration in gel anti tag western anti tag western blot anti tag western Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. DIP original experiment: DIP-111008X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. Only protein-protein interactions imex curation douglas-2008-6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column anti tag western anti tag western blot anti tag western Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. DIP original experiment: DIP-111009X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. Only protein-protein interactions imex curation douglas-2008-7 in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. DIP original experiment: DIP-111012X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas PM., Treusch S., Ren HY., Halfmann R., Duennwald ML., Lindquist S., Cyr DM. Only protein-protein interactions imex curation hsp71_yeast Heat shock protein SSA1 YAL005C SSA1 Heat shock protein YG100 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKAVGIDLGTTYSCVAHFANDRVDIIANDQGNRTTPSFVAFTDTERLIGDAAKNQAAMNPSNTVFDAKRLIGRNFNDPEVQADMKHFPFKLIDVDGKPQIQVEFKGETKNFTPEQISSMVLGKMKETAESYLGAKVNDAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKGKEEHVLIFDLGGGTFDVSLLSIEDGIFEVKATAGDTHLGGEDFDNRLVNHFIQEFKRKNKKDLSTNQRALRRLRTACERAKRTLSSSAQTSVEIDSLFEGIDFYTSITRARFEELCADLFRSTLDPVEKVLRDAKLDKSQVDEIVLVGGSTRIPKVQKLVTDYFNGKEPNRSINPDEAVAYGAAVQAAILTGDESSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNSTIPTKKSEIFSTYADNQPGVLIQVFEGERAKTKDNNLLGKFELSGIPPAPRGVPQIEVTFDVDSNGILNVSAVEKGTGKSNKITITNDKGRLSKEDIEKMVAEAEKFKEEDEKESQRIASKNQLESIAYSLKNTISEAGDKLEQADKDTVTKKAEETISWLDSNTTASKEEFDDKLKELQDIANPIMSKLYQAGGAPGGAAGGAPGGFPGGAPPAPEAEGPTVEEVD 249D8A3BC42527CA rnq1_yeast [PIN+] prion protein RNQ1 RNQ1 YCL028W YCL28W YCL181 Rich in asparagine and glutamine protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDTDKLISEAESHFSQGNHAEAVAKLTSAAQSNPNDEQMSTIESLIQKIAGYVMDNRSGGSDASQDRAAGGGSSFMNTLMADSKGSSQTQLGKLALLATVMTHSSNKGSSNRGFDVGTVMSMLSGSGGGSQSMGASGLAALASQFFKSGNNSQGQGQGQGQGQGQGQGQGQGSFTALASLASSFMNSNNNNQQGQNQSSGGSSFGALASMASSFMHSNNNQNSNNSQQGYNQSYQNGNQNSQGYNNQQYQGGNGGYQQQQGQSGGAFSSLASMAQSYLGGGQTQSNQQQYNQQGQNNQQQYQQQGQNYQHQQQGQQQQQGHSSSFSALASMASSYLGNNSNSNSSYGGQQQANEYGRPQQNGQQQSNEYGRPQYGGNQNSNGQHESFNFSGNFSQQNNNGNQNRY 8ED9C40A7DF70F7F sis1_yeast Protein SIS1 YNL007C SIS1 N2879 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVKETKLYDLLGVSPSANEQELKKGYRKAALKYHPDKPTGDTEKFKEISEAFEILNDPQKREIYDQYGLEAARSGGPSFGPGGPGGAGGAGGFPGGAGGFSGGHAFSNEDAFNIFSQFFGGSSPFGGADDSGFSFSSYPSGGGAGMGGMPGGMGGMHGGMGGMPGGFRSASSSPTYPEEETVQVNLPVSLEDLFVGKKKSFKIGRKGPHGASEKTQIDIQLKPGWKAGTKITYKNQGDYNPQTGRRKTLQFVIQEKSHPNFKRDGDDLIYTLPLSFKESLLGFSKTIQTIDGRTLPLSRVQPVQPSQTSTYPGQGMPTPKNPSQRGNLIVKYKVDYPISLNDAQKRAIDENF 642D3709C0FD5682 rnq1-ssa1-1 3171466 N/A 3171473 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3171474 unspecified role unspecified role bait bait Region 82-106 Region 82-106 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false rnq1-1 3171467 N/A 3171474 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rnq1-2 3171468 N/A 3171474 unspecified role unspecified role unspecified role unspecified role YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false sis1-rnq1-1 3171469 N/A 3171474 unspecified role unspecified role prey prey P25367:p.Leu94Ala L94A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3171475 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rnq1-3 3171470 N/A 3171474 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false rnq1-4 3171471 N/A 3171474 unspecified role unspecified role unspecified role unspecified role YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rnq1-sis1-1 3171472 N/A 3171474 unspecified role unspecified role bait bait Region 84-101 Region 84-101 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3171475 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false