DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sundvall-2008-1 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-68538X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-2 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-68536X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-3 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-68540X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-4 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-68541X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-5 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-68543X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-6 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-68537X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-7 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-68539X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-8 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-68542X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-9 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-252557X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-10 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-252558X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation sundvall-2008-11 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. DIP original experiment: DIP-252559X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms. Sundvall M., Korhonen A., Paatero I., Gaudio E., Melino G., Croce CM., Aqeilan RI., Elenius K. Only protein-protein interactions imex curation erbb4_human Receptor tyrosine-protein kinase erbB-4 ERBB4 HER4 p180erbB4 Proto-oncogene-like protein c-ErbB-4 Tyrosine kinase-type cell surface receptor HER4 protein protein human Homo sapiens Human MKPATGLWVWVSLLVAAGTVQPSDSQSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEHNRDLSFLRSVREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRKDGNFGLQELGLKNLTEILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNLTLVSTNGSSGCGRCHKSCTGRCWGPTENHCQTLTRTVCAEQCDGRCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFNAKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCTKINGNLIFLVTGIHGDPYNAIEAIDPEKLNVFRTVREITGFLNIQSWPPNMTDFSVFSNLVTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFSTINQRIVIRDNRKAENCTAEGMVCNHLCSSDGCWGPGPDQCLSCRRFSRGRICIESCNLYDGEFREFENGSICVECDPQCEKMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDGLQGANSFIFKYADPDRECHPCHPNCTQGCNGPTSHDCIYYPWTGHSTLPQHARTPLIAAGVIGGLFILVIVGLTFAVYVRRKSIKKKRALRRFLETELVEPLTPSGTAPNQAQLRILKETELKRVKVLGSGAFGTVYKGIWVPEGETVKIPVAIKILNETTGPKANVEFMDEALIMASMDHPHLVRLLGVCLSPTIQLVTQLMPHGCLLEYVHEHKDNIGSQLLLNWCVQIAKGMMYLEERRLVHRDLAARNVLVKSPNHVKITDFGLARLLEGDEKEYNADGGKMPIKWMALECIHYRKFTHQSDVWSYGVTIWELMTFGGKPYDGIPTREIPDLLEKGERLPQPPICTIDVYMVMVKCWMIDADSRPKFKELAAEFSRMARDPQRYLVIQGDDRMKLPSPNDSKFFQNLLDEEDLEDMMDAEEYLVPQAFNIPPPIYTSRARIDSNRSEIGHSPPPAYTPMSGNQFVYRDGGFAAEQGVSVPYRAPTSTIPEAPVAQGATAEIFDDSCCNGTLRKPVAPHVQEDSSTQRYSADPTVFAPERSPRGELDEEGYMTPMRDKPKQEYLNPVEENPFVSRRKNGDLQALDNPEYHNASNGPPKAEDEYVNEPLYLNTFANTLGKAEYLKNNILSMPEKAKKAFDNPDYWNHSLPPRSTLQHPDYLQEYSTKYFYKQNGRIRPIVAENPEYLSEFSLKPGTVLPPPPYRHRNTVV q96j02-2 E3 ubiquitin-protein ligase Itchy homolog HECT-type E3 ubiquitin transferase Itchy homolog ITCH Atrophin-1-interacting protein 4 NFE2-associated polypeptide 1 protein protein human Homo sapiens Human MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG q15303-3 Receptor tyrosine-protein kinase erbB-4 ERBB4 HER4 Proto-oncogene-like protein c-ErbB-4 Tyrosine kinase-type cell surface receptor HER4 p180erbB4 protein protein human Homo sapiens Human MKPATGLWVWVSLLVAAGTVQPSDSQSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEHNRDLSFLRSVREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRKDGNFGLQELGLKNLTEILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNLTLVSTNGSSGCGRCHKSCTGRCWGPTENHCQTLTRTVCAEQCDGRCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFNAKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCTKINGNLIFLVTGIHGDPYNAIEAIDPEKLNVFRTVREITGFLNIQSWPPNMTDFSVFSNLVTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFSTINQRIVIRDNRKAENCTAEGMVCNHLCSSDGCWGPGPDQCLSCRRFSRGRICIESCNLYDGEFREFENGSICVECDPQCEKMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDGLQGANSFIFKYADPDRECHPCHPNCTQGCNGPTSHDCIYYPWTGHSTLPQHARTPLIAAGVIGGLFILVIVGLTFAVYVRRKSIKKKRALRRFLETELVEPLTPSGTAPNQAQLRILKETELKRVKVLGSGAFGTVYKGIWVPEGETVKIPVAIKILNETTGPKANVEFMDEALIMASMDHPHLVRLLGVCLSPTIQLVTQLMPHGCLLEYVHEHKDNIGSQLLLNWCVQIAKGMMYLEERRLVHRDLAARNVLVKSPNHVKITDFGLARLLEGDEKEYNADGGKMPIKWMALECIHYRKFTHQSDVWSYGVTIWELMTFGGKPYDGIPTREIPDLLEKGERLPQPPICTIDVYMVMVKCWMIDADSRPKFKELAAEFSRMARDPQRYLVIQGDDRMKLPSPNDSKFFQNLLDEEDLEDMMDAEEYLVPQAFNIPPPIYTSRARIDSNRNQFVYRDGGFAAEQGVSVPYRAPTSTIPEAPVAQGATAEIFDDSCCNGTLRKPVAPHVQEDSSTQRYSADPTVFAPERSPRGELDEEGYMTPMRDKPKQEYLNPVEENPFVSRRKNGDLQALDNPEYHNASNGPPKAEDEYVNEPLYLNTFANTLGKAEYLKNNILSMPEKAKKAFDNPDYWNHSLPPRSTLQHPDYLQEYSTKYFYKQNGRIRPIVAENPEYLSEFSLKPGTVLPPPPYRHRNTVV Proteolytical processing generates E4ICD2 (s80Cyt2). itch-erbb4-1 3169495 N/A 3169506 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3169507 unspecified role unspecified role bait bait GST-tag GST-tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Binding region 285-350 Binding region 285-350 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false erbb4-ub-1 3169496 N/A 3169506 unspecified role unspecified role bait bait chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3169508 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false erbb4-ub-2 3169497 N/A 3169506 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3169508 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false erbb4-itch-1 3169498 N/A 3169509 unspecified role unspecified role bait bait chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3169507 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false erbb4-ub-3 3169499 N/A 3169506 unspecified role unspecified role bait bait chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3169508 unspecified role unspecified role prey prey chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false erbb4-itch-2 3169500 N/A 3169506 unspecified role unspecified role bait bait Q15303:p.Tyr1056Phe Y1056F mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q15303:p.Pro1054Ala P1054A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3169507 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false erbb4-ub-4 3169501 N/A 3169509 unspecified role unspecified role bait bait chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3169508 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false itch-erbb4-2 3169502 N/A 3169506 unspecified role unspecified role prey prey chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3169507 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false erbb4-ub-5 3169503 N/A 3169506 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3169508 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false 1 nodeMerge04 2013-08-05 erbb4-ub-6 3169504 N/A 3169509 unspecified role unspecified role bait bait chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3169508 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false 1 nodeMerge04 2013-08-05 erbb4-itch-3 3169505 N/A 3169509 unspecified role unspecified role bait bait chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3169507 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false 1 nodeMerge04 2013-08-05