DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ roy-2008-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Roy S., Sharom JR., Houde C., Loisel TP., Vaillancourt JP., Shao W., Saleh M., Nicholson DW. DIP original experiment: DIP-68519X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Confinement of caspase-12 proteolytic activity to autoprocessing. Roy S., Sharom JR., Houde C., Loisel TP., Vaillancourt JP., Shao W., Saleh M., Nicholson DW. Only protein-protein interactions imex curation roy-2008-2 in vitro In vitro protease assay protease assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Roy S., Sharom JR., Houde C., Loisel TP., Vaillancourt JP., Shao W., Saleh M., Nicholson DW. DIP original experiment: DIP-68520X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Confinement of caspase-12 proteolytic activity to autoprocessing. Roy S., Sharom JR., Houde C., Loisel TP., Vaillancourt JP., Shao W., Saleh M., Nicholson DW. Only protein-protein interactions imex curation casp1_rat Caspase-1 Casp1 Il1bc Il1bce Interleukin-1 beta convertase Interleukin-1 beta-converting enzyme p45 protein protein rat Rattus norvegicus (Rat) Rat MADKVLRAKRKQFINSVSVGTINGLLDELLEKRVLNQEEMDTIKLANITVMEKARDLCDHVTKKGPRASQMFITYICNEDCYLAEILELQSGPSAETVFVTEDSKGGHPFSSETKEKLNKEGGAFPGPSGSLKFCPLEIAQKLWKENHSEIYPIMKTPTRTRLALIICNTDFQHLSRRVGADVDLREMKLLLQDLGYTVKVKENLTALEMTKELKEFAACPEHKTSDSTFLVFMSHGLQEGICGITYSNEVADILKVDTIFQMMNTLKCPSLKDKPKVIIIQACRGEKQGVVLLKDSVGNSEEGFLTDAIFEDDGIKKAHIEKDFIAFCSSTPDNVSWRHPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPDSRLQMPTTERVTLTKRFYLFPGH caspc_rat Caspase-12 Casp12 protein protein rat Rattus norvegicus (Rat) Rat MAAKRTHERDPIYKIKGLAKDMLDGVFDDLMDKNVLNGDELLKIGEGASLILSKAENLVESFFEKTEMAGKIFAGHIANSDKQLSLQFPSDDEEDELQKMFTPSSASESRGKVEDEEMEVNVGVAHASHLMLTVPQGIQSTEVQDSLKLCSRDWFCTMKTERAEEIYPVMEKEGRTRLALIICNKKFDYLFDRDDAETDILNMKELLQNLGYSVVIKENLTAQEMETELMKFAGRPEHQSSDSTFLVFMSHGILEGICGVKHRNKKPDVLHDDTIFTIFNNSNCPSLRNKPKILIMQACRGRHTGTIWVSTSKGIATADTDEECVLSHRWNNSITKAHVETDFIAFKSSTPHNISWKVGKSGSLFISKLIDCFKKYCWCYHLEEIFRKVQYSFEVPGELTQMPTIERVSMTRYFYLFPGN casp1-casp12-2 3169258 N/A 3169260 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3169261 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false casp12-casp12-1 3169259 N/A 3169261 enzyme target enzyme target substrate substrate unspecified role unspecified role Q920D5:p.Asp319Ala D319A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3169261 enzyme enzyme unspecified role unspecified role Q920D5:p.Cys299Ala C299A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false