DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ nam-2008-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Nam TW., Jung HI., An YJ., Park YH., Lee SH., Seok YJ., Cha SS. DIP original experiment: DIP-68511X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Analyses of Mlc-IIBGlc interaction and a plausible molecular mechanism of Mlc inactivation by membrane sequestration. Nam TW., Jung HI., An YJ., Park YH., Lee SH., Seok YJ., Cha SS. Only protein-protein interactions imex curation nam-2008-2 in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Nam TW., Jung HI., An YJ., Park YH., Lee SH., Seok YJ., Cha SS. DIP original experiment: DIP-68514X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Analyses of Mlc-IIBGlc interaction and a plausible molecular mechanism of Mlc inactivation by membrane sequestration. Nam TW., Jung HI., An YJ., Park YH., Lee SH., Seok YJ., Cha SS. Only protein-protein interactions imex curation nam-2008-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nam TW., Jung HI., An YJ., Park YH., Lee SH., Seok YJ., Cha SS. DIP original experiment: DIP-68512X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Analyses of Mlc-IIBGlc interaction and a plausible molecular mechanism of Mlc inactivation by membrane sequestration. Nam TW., Jung HI., An YJ., Park YH., Lee SH., Seok YJ., Cha SS. Only protein-protein interactions imex curation nam-2008-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Nam TW., Jung HI., An YJ., Park YH., Lee SH., Seok YJ., Cha SS. DIP original experiment: DIP-68513X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Analyses of Mlc-IIBGlc interaction and a plausible molecular mechanism of Mlc inactivation by membrane sequestration. Nam TW., Jung HI., An YJ., Park YH., Lee SH., Seok YJ., Cha SS. Only protein-protein interactions imex curation mlc_ecoli Protein mlc Making large colonies protein mlc b1594 JW1586 protein protein ecoli Escherichia coli (strain K12) MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAPASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALRDLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTENGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQVVIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILELAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFNPQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKDAMYNGSLLIRLLQG 423608797FC980CB grx2eiib Grx2-EIIB protein protein ecolx Escherichia coli MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVGQKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLLPRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVKPNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAIMFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNH ptgcb_ecoli PTS system glucose-specific EIICB component ptsG glcA umg b1101 EIICB-Glc JW1087 protein protein ecoli Escherichia coli (strain K12) MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNH D97A80FD64B74F73 mlc-1 3168763 N/A 3168767 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false grx2eiib-mlc-1 3168764 N/A 3168768 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3168767 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false eiib-mlc-1 3168765 N/A 3168769 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3168767 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false eiib-mlc-2 3168766 N/A 3168769 unspecified role unspecified role bait bait P69786:p.Val449Gln V449Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P69786:p.Gln456Ala Q456A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P69786:p.Ala451Phe A451F mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3168767 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false