DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kittler-2008-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68375X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions imex curation kittler-2008-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68370X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions imex curation kittler-2008-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68373X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions imex curation kittler-2008-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68371X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions imex curation kittler-2008-5 in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68376X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions imex curation kittler-2008-6 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68374X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions imex curation kittler-2008-7 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68372X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions imex curation yeclphos GABA(A) receptor subunit gamma-2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ATHLQERDEEYGYECLDGKDC ap2m1_rat AP-2 complex subunit mu Adaptor protein complex AP-2 subunit mu AP-2 mu chain Ap2m1 Mu2-adaptin Plasma membrane adaptor AP-2 50 kDa protein Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 Clathrin assembly protein complex 2 mu medium chain Adaptor-related protein complex 2 subunit mu protein protein rat Rattus norvegicus (Rat) Rat MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC deeygyecld GABA(A) receptor subunit gamma-2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DEEYGYECLD yeclpep GABA(A) receptor subunit gamma-2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EYGYECLDG ygyecl GABA(A) receptor subunit gamma-2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) YGYECL d3zuy8_rat AP-2 complex subunit alpha Ap2a1 Ap2a1_predicted rCG_54084 protein protein rat Rattus norvegicus (Rat) Rat MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSNVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMTLLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQSFLPTVVHPGDLQTHLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF 5704048BEC825FDF ap2a2_rat AP-2 complex subunit alpha-2 Adaptor protein complex AP-2 subunit alpha-2 Alpha2-adaptin Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit Alpha-adaptin C Clathrin assembly protein complex 2 alpha-C large chain 100 kDa coated vesicle protein C Ap2a2 Adtab Adaptor-related protein complex 2 subunit alpha-2 protein protein rat Rattus norvegicus (Rat) Rat MPAVSKGEGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVINIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF yeclphos-ap2m1-1 3168550 N/A 3168557 unspecified role unspecified role bait bait in vitro In vitro N/A 3168558 unspecified role unspecified role prey prey in vitro In vitro direct interaction direct interaction false false false ap2m1-deeygyecld-1 3168551 N/A 3168559 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3168558 unspecified role unspecified role unspecified role unspecified role Region 158-435 Region 158-435 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false yeclpep-ap2m1-1 3168552 N/A 3168560 unspecified role unspecified role bait bait in vitro In vitro N/A 3168558 unspecified role unspecified role prey prey Region 156-435 Region 156-435 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ygyecl-ap2m1-1 3168553 N/A 3168561 unspecified role unspecified role bait bait in vitro In vitro N/A 3168558 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false yeclphos-ap2 alpha1-1 3168554 N/A 3168562 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat N/A 3168563 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat N/A 3168557 unspecified role unspecified role bait bait in vitro In vitro N/A 3168558 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat association association false false false yeclphos-ap2m1-2 3168555 N/A 3168557 unspecified role unspecified role bait bait in vitro In vitro N/A 3168558 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat physical association physical association false false false yeclpep-ap2m1-2 3168556 N/A 3168560 unspecified role unspecified role bait bait in vitro In vitro N/A 3168558 unspecified role unspecified role prey prey Region 158-435 Region 158-435 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false