UniProt UniProt http://www.uniprot.org shinohara-2008-3 yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shinohara M., Oh SD., Hunter N., Shinohara A. ashino@protein.osaka-u.ac.jp Nature genetics 2008 Only protein-protein interactions imex curation imex curation shinohara-2008-4 yeast Saccharomyces cerevisiae Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Shinohara M., Oh SD., Hunter N., Shinohara A. ashino@protein.osaka-u.ac.jp Nature genetics 2008 Only protein-protein interactions imex curation imex curation spo22_yeast Sporulation-specific protein 22 YIL073C SPO22 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDHNVNSTFRKTLVELCETATWITSQVYAAKNLEKNDLITVDNKISALYPIAEKYDRSFRTTTVILDEELILKLENAASSLWNSLTIAMKAEKASDKYFNEVFCKCKIFATKLLSIHEALFRTNTNLLRNFKCYISSFKSASEYRFDDLITNTQQHSEKYLQIINENVESFSNEEKTEFKKLTFEFYLVNFQLYLSENDLDTANIYTAKVNITDNSKYMDADLLIELCRMIYNSTVMLKEINNPETQLVDVNIISFLKDVEKYLELPVENLKSHTDYSNLKYSVLIFMANCLVEGHPQASELEQCDHYLSLLQNEYPNKVDPFILAINLTKRRNIVNPAETIEEILMRMIMSVDVISNFQAVIASINDLSKMNTKFSIVCLDYLLINKLNSKNDSKFLGKAICSRFLITTQSKTMNDSEIAESLENFSTQMERIVSEPLTKHAISCIITLLWNTGKKLEKMEKYVVSIRFYKLALKDIISQNYSDRGKIQRALQVVYNKIEDYSNTVRVYQDMDEVDRQSPLCQLLMLQSFLADDKTEEALTCLQKIKSSEDEKSTDALILAVAECKRKTDLSVQGLLMIFDKLQSKSNSQTISSTSSSQTLSILRYTLQMIVKVSEEEPLETFINYLPTVQKLLQKAVEFLKTVKLLNQLPPDVEKEAIYQQSVAVNEIEWFASFSYNVAVKCLVDQSCESISEFPQYCIQFIDLIPVQDFTFPKMYHFTYWRFKATILQLIIAKEKAKQDQHQKDWDIYEKSEELVNSINVMKKSSEFKDGSSLEDRNTLHECFLEALTIHLESALMMPDQTRILDILKKTELYQDSRVDALLIDISSNMEDLPKGVLIEILETVLKRNMGPEVKERELCSWLRILLENAINLNHEVELRILDRVLKILNINQSSLQDTDGVLQTELETIATYCWNIGVNYIIKDNKSNGIVWCKHSMGFANMVNEGLQEQLYSLWESLASSANIDINSIAK spo16_yeast Sporulation-specific protein 16 SPO16 YHR153C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEFFWDVQKIQEISNVEEHSVVKCVTVNTSRLISQLNEELQDEESGVNFIVTQLQLLINNVYEKIQKSPGVPAHRSLMINLNFTRLKFSIAYWDILLERSLDLINGPSKTGARYFITEVTPVDRSRYVENNQYFLAFKANQRLTRNSVDMDEFIDFEILIKQIIFDLFKKNGIPDQDFEAILSRFHNLESLVVAFNE spo16-spo22-1 3321266 N/A 3321268 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3321269 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5C Interaction observed in CST9, ZIP2 and MSH4 -/- cells spo16-spo22-2 3321267 N/A 3321268 unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3321269 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 5D Interaction observed in CST9, ZIP2 and MSH4 -/- cells