DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kwon-2008-8 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kwon C., Neu C., Pajonk S., Yun HS., Lipka U., Humphry M., Bau S., Straus M., Kwaaitaal M., Rampelt H., El Kasmi F., Jürgens G., Parker J., Panstruga R., Lipka V., Schulze-Lefert P. DIP original experiment: DIP-250285X Nature 2008 IMEx Co-option of a default secretory pathway for plant immune responses. Kwon C., Neu C., Pajonk S., Yun HS., Lipka U., Humphry M., Bau S., Straus M., Kwaaitaal M., Rampelt H., El Kasmi F., Jürgens G., Parker J., Panstruga R., Lipka V., Schulze-Lefert P. Only protein-protein interactions imex curation kwon-2008-9 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kwon C., Neu C., Pajonk S., Yun HS., Lipka U., Humphry M., Bau S., Straus M., Kwaaitaal M., Rampelt H., El Kasmi F., Jürgens G., Parker J., Panstruga R., Lipka V., Schulze-Lefert P. DIP original experiment: DIP-250287X Nature 2008 IMEx Co-option of a default secretory pathway for plant immune responses. Kwon C., Neu C., Pajonk S., Yun HS., Lipka U., Humphry M., Bau S., Straus M., Kwaaitaal M., Rampelt H., El Kasmi F., Jürgens G., Parker J., Panstruga R., Lipka V., Schulze-Lefert P. Only protein-protein interactions imex curation kwon-2008-10 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kwon C., Neu C., Pajonk S., Yun HS., Lipka U., Humphry M., Bau S., Straus M., Kwaaitaal M., Rampelt H., El Kasmi F., Jürgens G., Parker J., Panstruga R., Lipka V., Schulze-Lefert P. DIP original experiment: DIP-250288X Nature 2008 IMEx Co-option of a default secretory pathway for plant immune responses. Kwon C., Neu C., Pajonk S., Yun HS., Lipka U., Humphry M., Bau S., Straus M., Kwaaitaal M., Rampelt H., El Kasmi F., Jürgens G., Parker J., Panstruga R., Lipka V., Schulze-Lefert P. Only protein-protein interactions imex curation kwon-2008-11 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kwon C., Neu C., Pajonk S., Yun HS., Lipka U., Humphry M., Bau S., Straus M., Kwaaitaal M., Rampelt H., El Kasmi F., Jürgens G., Parker J., Panstruga R., Lipka V., Schulze-Lefert P. DIP original experiment: DIP-250286X Nature 2008 IMEx Co-option of a default secretory pathway for plant immune responses. Kwon C., Neu C., Pajonk S., Yun HS., Lipka U., Humphry M., Bau S., Straus M., Kwaaitaal M., Rampelt H., El Kasmi F., Jürgens G., Parker J., Panstruga R., Lipka V., Schulze-Lefert P. Only protein-protein interactions imex curation sy121_arath Syntaxin-121 SYP121 SYR1 Syntaxin-related protein At-Syr1 F26K24.11 At3g11820 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNDLFSSSFSRFRSGEPSPRRDVAGGGDGVQMANPAGSTGGVNLDKFFEDVESVKEELKELDRLNETLSSCHEQSKTLHNAKAVKDLRSKMDGDVGVALKKAKMIKVKLEALDRANAANRSLPGCGPGSSSDRTRTSVLNGLRKKLMDSMDSFNRLRELISSEYRETVQRRYFTVTGENPDERTLDRLISTGESERFLQKAIQEQGRGRVLDTINEIQERHDAVKDIEKNLRELHQVFLDMAVLVEHQGAQLDDIESHVGRASSFIRGGTDQLQTARVYQKNTRKWTCIAIIILIIIITVVVLAVLKPWNNSSGGGGGGGGGGTTGGSQPNSGTPPNPPQARRLLR va722_arath Vesicle-associated membrane protein 722 VAMP722 HAT24 SAR1 Synaptobrevin-related protein 1 F25I18.14 At2g33120 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVENGFTYCVVAVDSAGRQIPMAFLERVKEDFNKRYGGGKAATAQANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIELLVDKTENLRSQAQDFRTQGTQMRRKMWFQNMKIKLIVLAIIIALILIIILSICGGFNCGK snp33_arath SNAP25 homologous protein SNAP33 Synaptosomal-associated protein SNAP25-like 1 Snap25a SNAP33 SNAP33B MAF19.21 At5g61210 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MFGLRKSPANLPKHNSVDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLADMTNPFGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSKTWKPKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRGRRLLGK snap33-syp121-1 3167579 N/A 3167583 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3167584 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3167585 unspecified role unspecified role bait bait GST-tag GST-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false snap33-syp121-2 3167580 N/A 3167583 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3167585 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false syp121-snap33-1 3167581 N/A 3167583 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3167585 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false snap33-syp121-3 3167582 N/A 3167583 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3167585 unspecified role unspecified role bait bait GST-tag GST-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false