DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wu-2008-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wu ZQ., Liu X. DIP original experiment: DIP-68264X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing. Wu ZQ., Liu X. Only protein-protein interactions imex curation wu-2008-4 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wu ZQ., Liu X. DIP original experiment: DIP-68265X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing. Wu ZQ., Liu X. Only protein-protein interactions imex curation wu-2008-5 helpy Helicobacter pylori Campylobacter pylori anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wu ZQ., Liu X. DIP original experiment: DIP-68267X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing. Wu ZQ., Liu X. Only protein-protein interactions imex curation wu-2008-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Wu ZQ., Liu X. DIP original experiment: DIP-68269X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing. Wu ZQ., Liu X. Only protein-protein interactions imex curation wu-2008-7 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Wu ZQ., Liu X. DIP original experiment: DIP-68268X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing. Wu ZQ., Liu X. Only protein-protein interactions imex curation wu-2008-8 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wu ZQ., Liu X. DIP original experiment: DIP-68266X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing. Wu ZQ., Liu X. Only protein-protein interactions imex curation kat7_human Histone acetyltransferase KAT7 KAT7 HBO1 HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 Histone acetyltransferase binding to ORC1 MYST2 Lysine acetyltransferase 7 protein protein human Homo sapiens Human MPRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSARVTRSSARLSQSSQDSSPVRNLQSFGTEEPAYSTRRVTRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRETKNTADHDESPPRTPTGNAPSSESDIDISSPNVSHDESIAKDMSLKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSLGHLTGKHERHFSISGCPLYHNLSADECKVRAQSRDKQIEERMLSHRQDDNNRHATRHQAPTERQLRYKEKVAELRKKRNSGLSKEQKEKYMEHRQTYGNTREPLLENLTSEYDLDLFRRAQARASEDLEKLRLQGQITEGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDPSCLKWTPPKGT plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS plk1-kat7-1 3167139 N/A 3167145 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3167146 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false plk1-kat7-2 3167140 N/A 3167145 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro N/A 3167146 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro direct interaction direct interaction false false false plk1-kat7-3 3167141 N/A 3167145 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3167146 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false plk1-kat7-4 3167142 N/A 3167145 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O95251:p.[Thr85Ala;Thr88Ala] T85A/T88A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3167146 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false kat7-plk1-1 3167143 N/A 3167145 unspecified role unspecified role bait bait O95251:p.[Thr85Ala;Thr88Ala] T85A/T88A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3167146 unspecified role unspecified role prey prey Region 417-584 Region 417-584 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false kat7-plk1-2 3167144 N/A 3167145 enzyme target enzyme target substrate substrate unspecified role unspecified role O95251:p.Ser57Ala S57A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3167146 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false