DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lee-2008-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lee J., Thompson JR., Botuyan MV., Mer G. DIP original experiment: DIP-113696X Nature structural & molecular biology 2008 IMEx Distinct binding modes specify the recognition of methylated histones H3K4 and H4K20 by JMJD2A-tudor. Lee J., Thompson JR., Botuyan MV., Mer G. Only protein-protein interactions imex curation lee-2008-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lee J., Thompson JR., Botuyan MV., Mer G. DIP original experiment: DIP-113694X Nature structural & molecular biology 2008 IMEx Distinct binding modes specify the recognition of methylated histones H3K4 and H4K20 by JMJD2A-tudor. Lee J., Thompson JR., Botuyan MV., Mer G. Only protein-protein interactions imex curation lee-2008-4 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lee J., Thompson JR., Botuyan MV., Mer G. DIP original experiment: DIP-113695X Nature structural & molecular biology 2008 IMEx Distinct binding modes specify the recognition of methylated histones H3K4 and H4K20 by JMJD2A-tudor. Lee J., Thompson JR., Botuyan MV., Mer G. Only protein-protein interactions imex curation h4k20me3 Histone 4 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KRHRKVLRDN o75164-1 Lysine-specific demethylase 4A [histone H3]-trimethyl-L-lysine(36) demethylase 4A [histone H3]-trimethyl-L-lysine(9) demethylase 4A KDM4A JHDM3A JMJD2 JMJD2A KIAA0677 JmjC domain-containing histone demethylation protein 3A Jumonji domain-containing protein 2A protein protein human Homo sapiens Human MASESETLNPSARIMTFYPTMEEFRNFSRYIAYIESQGAHRAGLAKVVPPKEWKPRASYDDIDDLVIPAPIQQLVTGQSGLFTQYNIQKKAMTVREFRKIANSDKYCTPRYSEFEELERKYWKNLTFNPPIYGADVNGTLYEKHVDEWNIGRLRTILDLVEKESGITIEGVNTPYLYFGMWKTSFAWHTEDMDLYSINYLHFGEPKSWYSVPPEHGKRLERLAKGFFPGSAQSCEAFLRHKMTLISPLMLKKYGIPFDKVTQEAGEFMITFPYGYHAGFNHGFNCAESTNFATRRWIEYGKQAVLCSCRKDMVKISMDVFVRKFQPERYKLWKAGKDNTVIDHTLPTPEAAEFLKESELPPRAGNEEECPEEDMEGVEDGEEGDLKTSLAKHRIGTKRHRVCLEIPQEVSQSELFPKEDLSSEQYEMTECPAALAPVRPTHSSVRQVEDGLTFPDYSDSTEVKFEELKNVKLEEEDEEEEQAAAALDLSVNPASVGGRLVFSGSKKKSSSSLGSGSSRDSISSDSETSEPLSCRAQGQTGVLTVHSYAKGDGRVTVGEPCTRKKGSAARSFSERELAEVADEYMFSLEENKKSKGRRQPLSKLPRHHPLVLQECVSDDETSEQLTPEEEAEETEAWAKPLSQLWQNRPPNFEAEKEFNETMAQQAPHCAVCMIFQTYHQVEFGGFNQNCGNASDLAPQKQRTKPLIPEMCFTSTGCSTDINLSTPYLEEDGTSILVSCKKCSVRVHASCYGVPPAKASEDWMCSRCSANALEEDCCLCSLRGGALQRANDDRWVHVSCAVAILEARFVNIAERSPVDVSKIPLPRFKLKCIFCKKRRKRTAGCCVQCSHGRCPTAFHVSCAQAAGVMMQPDDWPFVVFITCFRHKIPNLERAKGALQSITAGQKVISKHKNGRFYQCEVVRLTTETFYEVNFDDGSFSDNLYPEDIVSQDCLQFGPPAEGEVVQVRWTDGQVYGAKFVASHPIQMYQVEFEDGSQLVVKRDDVYTLDEELPKRVKSRLSVASDMRFNEIFTEKEVKQEKKRQRVINSRYREDYIEPALYRAIME h3k4me3 Histone 3 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKS h4k20me3-kdm4a-1 3165507 N/A 3165510 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3165511 unspecified role unspecified role unspecified role unspecified role Region 897-1011 Region 897-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h4k20me3-kdm4a-2 3165508 N/A 3165510 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3165511 unspecified role unspecified role unspecified role unspecified role Region 897-1011 Region 897-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false O75164-1:p.Asp939Arg D939R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3k4me3-kdm4a-1 3165509 N/A 3165512 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3165511 unspecified role unspecified role unspecified role unspecified role Region 897-1011 Region 897-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false O75164-1:p.Asp945Arg D945R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false