DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhai-2008-1 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. DIP original experiment: DIP-113683X Nature structural & molecular biology 2008 IMEx Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV. Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. Only protein-protein interactions imex curation zhai-2008-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. DIP original experiment: DIP-113688X Nature structural & molecular biology 2008 IMEx Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV. Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. Only protein-protein interactions imex curation zhai-2008-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. DIP original experiment: DIP-113686X Nature structural & molecular biology 2008 IMEx Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV. Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. Only protein-protein interactions imex curation zhai-2008-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. DIP original experiment: DIP-113684X Nature structural & molecular biology 2008 IMEx Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV. Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. Only protein-protein interactions imex curation zhai-2008-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. DIP original experiment: DIP-113687X Nature structural & molecular biology 2008 IMEx Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV. Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. Only protein-protein interactions imex curation zhai-2008-6 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. DIP original experiment: DIP-113685X Nature structural & molecular biology 2008 IMEx Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV. Zhai Q., Fisher RD., Chung HY., Myszka DG., Sundquist WI., Hill CP. Only protein-protein interactions imex curation p6gaga HIV-1 p6GagA peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DKELYPLASLRSLFG pdc6i_human Programmed cell death 6-interacting protein PDCD6IP AIP1 KIAA1375 ALIX ALG-2-interacting protein 1 Hp95 ALG-2-interacting protein X protein protein human Homo sapiens Human MATFISVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRAAVGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNLDNDEGLKIAAKHYQFASGAFLHIKETVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAKLANQAADYFGDAFKQCQYKDTLPKEVFPVLAAKHCIMQANAEYHQSILAKQQKKFGEEIARLQHAAELIKTVASRYDEYVNVKDFSDKINRALAAAKKDNDFIYHDRVPDLKDLDPIGKATLVKSTPVNVPISQKFTDLFEKMVPVSVQQSLAAYNQRKADLVNRSIAQMREATTLANGVLASLNLPAAIEDVSGDTVPQSILTKSRSVIEQGGIQTVDQLIKELPELLQRNREILDESLRLLDEEEATDNDLRAKFKERWQRTPSNELYKPLRAEGTNFRTVLDKAVQADGQVKECYQSHRDTIVLLCKPEPELNAAIPSANPAKTMQGSEVVNVLKSLLSNLDEVKKEREGLENDLKSVNFDMTSKFLTALAQDGVINEEALSVTELDRVYGGLTTKVQESLKKQEGLLKNIQVSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKTERDELLKDLQQSIAREPSAPSIPTPAYQSSPAGGHAPTPPTPAPRTMPPTKPQPPARPPPPVLPANRAPSATAPSPVGAGTAAPAPSQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQSPGQAPYPGPQQPSYPFPQPPQQSYYPQQ p9gag EIAV p9Gag peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) TQNLYPDLSEIKK p6gagt HIV-1 p6GagT peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DKELYPLTSLRSLFG pdcd6ip-p6gaga-1 3165216 N/A 3165222 unspecified role unspecified role prey prey in vitro In vitro N/A 3165223 unspecified role unspecified role bait bait Region 1-698 Region 1-698 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false p9gag-pdcd6ip-1 3165217 N/A 3165224 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3165223 unspecified role unspecified role unspecified role unspecified role Region 1-698 Region 1-698 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false p6gaga-pdcd6ip-1 3165218 N/A 3165222 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3165223 unspecified role unspecified role unspecified role unspecified role Region 1-698 Region 1-698 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pdcd6ip-p6gagt-1 3165219 N/A 3165225 unspecified role unspecified role prey prey in vitro In vitro N/A 3165223 unspecified role unspecified role bait bait Region 1-698 Region 1-698 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false p6gagt-pdcd6ip-1 3165220 N/A 3165225 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3165223 unspecified role unspecified role unspecified role unspecified role Region 1-698 Region 1-698 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pdcd6ip-p9gag-1 3165221 N/A 3165224 unspecified role unspecified role prey prey in vitro In vitro N/A 3165223 unspecified role unspecified role bait bait Region 1-698 Region 1-698 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false