DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ diao-2008-1 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Diao J., Zhang Y., Huibregtse JM., Zhou D., Chen J. DIP original experiment: DIP-113678X Nature structural & molecular biology 2008 IMEx Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase. Diao J., Zhang Y., Huibregtse JM., Zhou D., Chen J. Only protein-protein interactions imex curation diao-2008-2 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Diao J., Zhang Y., Huibregtse JM., Zhou D., Chen J. DIP original experiment: DIP-113680X Nature structural & molecular biology 2008 IMEx Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase. Diao J., Zhang Y., Huibregtse JM., Zhou D., Chen J. Only protein-protein interactions imex curation diao-2008-3 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Diao J., Zhang Y., Huibregtse JM., Zhou D., Chen J. DIP original experiment: DIP-113679X Nature structural & molecular biology 2008 IMEx Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase. Diao J., Zhang Y., Huibregtse JM., Zhou D., Chen J. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG sopa_salty E3 ubiquitin-protein ligase SopA sopA HECT-type E3 ubiquitin transferase SopA Salmonella outer protein A Secreted effector protein SopA STM2066 protein protein salty Salmonella typhimurium MKISSGAINFSTIPNQVKKLITSIREHTKNGLTSKITSVKNTHTSLNEKFKTGKDSPIEFALPQKIKDFFQPKDKNTLNKTLITVKNIKDTNNAGKKNISAEDVSKMNAAFMRKHIANQTCDYNYRMTGAAPLPGGVSVSANNRPTVSEGRTPPVSPSLSLQATSSPSSPADWAKKLTDAVLRQKAGETLTAADRDFSNADFRNITFSKILPPSFMERDGDIIKGFNFSNSKFTYSDISHLHFDECRFTYSTLSDVVCSNTKFSNSDMNEVFLQYSITTQQQPSFIDTTLKNTLIRHKANLSGVILNEPDNSSPPSVSGGGNFIRLGDIWLQMPLLWTENAVDGFLNHEHNNGKSILMTIDSLPDKYSQEKVQAMEDLVKSLRGGRLTEACIRPVESSLVSVLAHPPYTQSALISEWLGPVQERFFAHQCQTYNDVPLPAPDTYYQQRILPVLLDSFDRNSAAMTTHSGLFNQVILHCMTGVDCTDGTRQKAAALYEQYLAHPAVSPHIHNGLFGNYDGSPDWTTRAADNFLLLSSQDSDTAMMLSTDTLLTMLNPTPDTAWDNFYLLRAGENVSTAQISPVELFRHDFPVFLAAFNQQATQRRFGELIDIILSTEEHGELNQQFLAATNQKHSTVKLIDDASVSRLATIFDPLLPEGKLSPAHYQHILSAYHLTDATPQKQAETLFCLSTAFARYSSSAIFGTEHDSPPALRGYAEALMQKAWELSPAIFPSSEQFTEWSDRFHGLHGAFTCTSVVADSMQRHARKYFPSVLSSILPLAWA 1D56B53EC05EDB94 p68036-1 Ubiquitin-conjugating enzyme E2 L3 E2 ubiquitin-conjugating enzyme L3 L-UBC UbcH7 Ubiquitin carrier protein L3 Ubiquitin-conjugating enzyme E2-F1 Ubiquitin-protein ligase L3 UBE2L3 UBCE7 UBCH7 protein protein human Homo sapiens Human MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD sopa-ub-1 3165021 N/A 3165024 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3165025 unspecified role unspecified role unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 470-782 Region 470-782 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sopa-ube2l3-1 3165022 N/A 3165026 unspecified role unspecified role unspecified role unspecified role P68036-1:p.Phe63Ala F63A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Fluorescein-label Fluorescein-label fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3165025 unspecified role unspecified role unspecified role unspecified role Region 370-782 Region 370-782 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sopa-ube2l3-2 3165023 N/A 3165026 unspecified role unspecified role prey prey P68036-1:p.Phe63Ala F63A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3165025 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 370-782 Region 370-782 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false