DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ xiang-2007-1 drome Drosophila melanogaster (Fruit fly) Fruit fly anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiang Y., Takeo S., Florens L., Hughes SE., Huo LJ., Gilliland WD., Swanson SK., Teeter K., Schwartz JW., Washburn MP., Jaspersen SL., Hawley RS. DIP original experiment: DIP-67870X PLoS biology 2007 IMEx The inhibition of polo kinase by matrimony maintains G2 arrest in the meiotic cell cycle. Xiang Y., Takeo S., Florens L., Hughes SE., Huo LJ., Gilliland WD., Swanson SK., Teeter K., Schwartz JW., Washburn MP., Jaspersen SL., Hawley RS. Only protein-protein interactions imex curation xiang-2007-2 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Xiang Y., Takeo S., Florens L., Hughes SE., Huo LJ., Gilliland WD., Swanson SK., Teeter K., Schwartz JW., Washburn MP., Jaspersen SL., Hawley RS. DIP original experiment: DIP-67872X PLoS biology 2007 IMEx The inhibition of polo kinase by matrimony maintains G2 arrest in the meiotic cell cycle. Xiang Y., Takeo S., Florens L., Hughes SE., Huo LJ., Gilliland WD., Swanson SK., Teeter K., Schwartz JW., Washburn MP., Jaspersen SL., Hawley RS. Only protein-protein interactions imex curation xiang-2007-3 drome Drosophila melanogaster (Fruit fly) Fruit fly anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiang Y., Takeo S., Florens L., Hughes SE., Huo LJ., Gilliland WD., Swanson SK., Teeter K., Schwartz JW., Washburn MP., Jaspersen SL., Hawley RS. DIP original experiment: DIP-67869X PLoS biology 2007 IMEx The inhibition of polo kinase by matrimony maintains G2 arrest in the meiotic cell cycle. Xiang Y., Takeo S., Florens L., Hughes SE., Huo LJ., Gilliland WD., Swanson SK., Teeter K., Schwartz JW., Washburn MP., Jaspersen SL., Hawley RS. Only protein-protein interactions imex curation xiang-2007-4 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xiang Y., Takeo S., Florens L., Hughes SE., Huo LJ., Gilliland WD., Swanson SK., Teeter K., Schwartz JW., Washburn MP., Jaspersen SL., Hawley RS. DIP original experiment: DIP-67871X PLoS biology 2007 IMEx The inhibition of polo kinase by matrimony maintains G2 arrest in the meiotic cell cycle. Xiang Y., Takeo S., Florens L., Hughes SE., Huo LJ., Gilliland WD., Swanson SK., Teeter K., Schwartz JW., Washburn MP., Jaspersen SL., Hawley RS. Only protein-protein interactions imex curation polo_drome Serine/threonine-protein kinase polo polo CG12306 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAAKPEDKSTDIPDRLVDINQRKTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHNQKEKTAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSITEFECRYYIYQIIQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEYRVPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKVPMFLPSSCLTMAPRIGSNDTIEDSMHRKPLMEMNGIRPDDTRLESTFLKANLHDAITASAQVCRHSEDYRSDIESLYQQLTNLINGKPRILQGNLGDENTDPAAQPLFWISKWVDYSDKYGFGYQLCDEGIGVMFNDTTKLILLPNQINVHFIDKDGKETYMTTTDYCKSLDKKMKLLSYFKRYMIEHLVKAGANNVNIESDQISRMPHLHSWFRTTCAVVMHLTNGSVQLNFSDHMKLILCPRMSAITYMDQEKNFRTYRFSTIVENGVSKDLYQKIRYAQEKLRKMLEKMFT mtrm_drome Protein matrimony mtrm anon-D52 CG18543 Cell cycle arrest protein D52 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MENSRTPTNKTKITLNRTPTLKERRWNTLKVNTSNVRCSTPIFGNFRSPNLSPIENMGTKGKSPVSPMRFATFKKVPTKVHPKQQQQQQHQHCHRTQLKPPPFVLPKPQEEIIEPEREIKSCSSPDTCSDDSNMETSLALESRRRSIKASNHSYVVNHAANVEQILMHMGLENYVTNFEEAHIDLVELASLERADLVKIGLNTDEDCNRIMDVLHTL polo-mtrm-1 3164890 N/A 3164894 unspecified role unspecified role bait bait drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3164895 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false mtrm-polo-1 3164891 N/A 3164894 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3164895 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false mtrm-polo-2 3164892 N/A 3164894 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3164895 unspecified role unspecified role bait bait Q23973:p.Thr40Ala T40A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false polo-mtrm-2 3164893 N/A 3164894 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3164895 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false