DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ stuchell_brereton-2007-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. DIP original experiment: DIP-253067X Nature 2007 IMEx ESCRT-III recognition by VPS4 ATPases. Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. Only protein-protein interactions imex curation stuchell_brereton-2007-2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. DIP original experiment: DIP-253065X Nature 2007 IMEx ESCRT-III recognition by VPS4 ATPases. Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. Only protein-protein interactions imex curation stuchell_brereton-2007-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. DIP original experiment: DIP-253061X Nature 2007 IMEx ESCRT-III recognition by VPS4 ATPases. Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. Only protein-protein interactions imex curation stuchell_brereton-2007-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. DIP original experiment: DIP-253063X Nature 2007 IMEx ESCRT-III recognition by VPS4 ATPases. Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. Only protein-protein interactions imex curation stuchell_brereton-2007-5 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. DIP original experiment: DIP-253068X Nature 2007 IMEx ESCRT-III recognition by VPS4 ATPases. Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. Only protein-protein interactions imex curation stuchell_brereton-2007-6 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. DIP original experiment: DIP-253066X Nature 2007 IMEx ESCRT-III recognition by VPS4 ATPases. Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. Only protein-protein interactions imex curation stuchell_brereton-2007-7 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. DIP original experiment: DIP-253064X Nature 2007 IMEx ESCRT-III recognition by VPS4 ATPases. Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. Only protein-protein interactions imex curation stuchell_brereton-2007-8 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. DIP original experiment: DIP-253062X Nature 2007 IMEx ESCRT-III recognition by VPS4 ATPases. Stuchell-Brereton MD., Skalicky JJ., Kieffer C., Karren MA., Ghaffarian S., Sundquist WI. Only protein-protein interactions imex curation q9uqn3-1 Charged multivesicular body protein 2b CHMP2B CHMP2.5 Chromatin-modifying protein 2b Vacuolar protein sorting-associated protein 2-2 CGI-84 protein protein human Homo sapiens Human MASLFKKKTVDDVIKEQNRELRGTQRAIIRDRAALEKQEKQLELEIKKMAKIGNKEACKVLAKQLVHLRKQKTRTFAVSSKVTSMSTQTKVMNSQMKMAGAMSTTAKTMQAVNKKMDPQKTLQTMQNFQKENMKMEMTEEMINDTLDDIFDGSDDEEESQDIVNQVLDEIGIEISGKMAKAPSAARSLPSASTSKATISDEEIERQLKALGVD vps4b_human Vacuolar protein sorting-associated protein 4B VPS4B SKD1 VPS42 Suppressor of K(+) transport growth defect 1 Cell migration-inducing gene 1 protein MIG1 protein protein human Homo sapiens Human MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG vps4a_human Vacuolar protein sorting-associated protein 4A VPS4-1 Protein SKD2 VPS4A VPS4 protein protein human Homo sapiens Human MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCVQYLDRAEKLKDYLRSKEKHGKKPVKENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDFGQES chm1b_human Charged multivesicular body protein 1b CHMP1B C18orf2 Chromatin-modifying protein 1b CHMP1.5 Vacuolar protein sorting-associated protein 46-2 protein protein human Homo sapiens Human MSNMEKHLFNLKFAAKELSRSAKKCDKEEKAEKAKIKKAIQKGNMEVARIHAENAIRQKNQAVNFLRMSARVDAVAARVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFETLDVQTQQMEDTMSSTTTLTTPQNQVDMLLQEMADEAGLDLNMELPQGQTGSVGTSVASAEQDELSQRLARLRDQV q9hd42-1 Charged multivesicular body protein 1a CHMP1A CHMP1 KIAA0047 PCOLN3 PRSM1 Chromatin-modifying protein 1a Vacuolar protein sorting-associated protein 46-1 protein protein human Homo sapiens Human MDDTLFQLKFTAKQLEKLAKKAEKDSKAEQAKVKKALLQKNVECARVYAENAIRKKNEGVNWLRMASRVDAVASKVQTAVTMKGVTKNMAQVTKALDKALSTMDLQKVSSVMDRFEQQVQNLDVHTSVMEDSMSSATTLTTPQEQVDSLIMQIAEENGLEVLDQLSQLPEGASAVGESSVRSQEDQLSRRLAALRN chmp2b-vps4b-1 3162494 N/A 3162502 unspecified role unspecified role unspecified role unspecified role Region 195-213 Region 195-213 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3162503 unspecified role unspecified role unspecified role unspecified role Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false vps4a-chmp2b-1 3162495 N/A 3162502 unspecified role unspecified role prey prey Region 195-213 Region 195-213 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3162504 unspecified role unspecified role bait bait Region 1-84 Region 1-84 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false chmp1b-vps4b-1 3162496 N/A 3162505 unspecified role unspecified role bait bait Region 180-196 Region 180-196 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q7LBR1:p.Leu192Ala L192A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q7LBR1:p.Leu188Ala L188A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3162503 unspecified role unspecified role prey prey Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false vps4b-chmp2b-1 3162497 N/A 3162502 unspecified role unspecified role prey prey Region 195-213 Region 195-213 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3162503 unspecified role unspecified role bait bait Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false chmp1b-vps4a-2 3162498 N/A 3162505 unspecified role unspecified role bait bait Region 180-196 Region 180-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3162504 unspecified role unspecified role prey prey Region 1-84 Region 1-84 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9UN37:p.Leu64Asp L64D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9UN37:p.Leu64Ala L64A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false chmp1a-vps4a-6 3162499 N/A 3162504 unspecified role unspecified role unspecified role unspecified role Region 1-84 Region 1-84 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3162506 unspecified role unspecified role unspecified role unspecified role Region 180-196 Region 180-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false vps4a-chmp1a-4 3162500 N/A 3162504 unspecified role unspecified role bait bait Region 1-84 Region 1-84 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3162506 unspecified role unspecified role prey prey Region 180-196 Region 180-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false vps4b-chmp1a-3 3162501 N/A 3162506 unspecified role unspecified role prey prey Region 180-196 Region 180-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3162503 unspecified role unspecified role bait bait Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false