DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hayashi-2007-7 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255074X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation hayashi-2007-8 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255076X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation hayashi-2007-9 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255082X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation hayashi-2007-10 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255080X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation hayashi-2007-11 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255078X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation hayashi-2007-12 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255083X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation hayashi-2007-13 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255075X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation hayashi-2007-14 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255079X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation hayashi-2007-15 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255081X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation hayashi-2007-16 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hayashi I., Plevin MJ., Ikura M. DIP original experiment: DIP-255077X Nature structural & molecular biology 2007 IMEx CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Hayashi I., Plevin MJ., Ikura M. Only protein-protein interactions imex curation p30622-1 CAP-Gly domain-containing linker protein 1 CLIP1 CYLN1 Cytoplasmic linker protein 1 Cytoplasmic linker protein 170 alpha-2 RSN Reed-Sternberg intermediate filament-associated protein Restin Long protein protein human Homo sapiens Human MSMLKPSGLKAPTKILKPGSTALKTPTAVVAPVEKTISSEKASSTPSSETQEEFVDDFRVGERVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTRPSKLTRKVQAEDEANGLQTTPASRATSPLCTSTASMVSSSPSTPSNIPQKPSQPAAKEPSATPPISNLTKTASESISNLSEAGSIKKGERELKIGDRVLVGGTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKIGFPSTTPAKAKANAVRRVMATTSASLKRSPSASSLSSMSSVASSVSSRPSRTGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHVGEIEQELALARDGHDQHVLELEAKMDQLRTMVEAADREKVELLNQLEEEKRKVEDLQFRVEEESITKGDLETQTKLEHARIKELEQSLLFEKTKADKLQRELEDTRVATVSEKSRIMELEKDLALRVQEVAELRRRLESNKPAGDVDMSLSLLQEISSLQEKLEVTRTDHQREITSLKEHFGAREETHQKEIKALYTATEKLSKENESLKSKLEHANKENSDVIALWKSKLETAIASHQQAMEELKVSFSKGLGTETAEFAELKTQIEKMRLDYQHEIENLQNQQDSERAAHAKEMEALRAKLMKVIKEKENSLEAIRSKLDKAEDQHLVEMEDTLNKLQEAEIKVKELEVLQAKCNEQTKVIDNFTSQLKATEEKLLDLDALRKASSEGKSEMKKLRQQLEAAEKQIKHLEIEKNAESSKASSITRELQGRELKLTNLQENLSEVSQVKETLEKELQILKEKFAEASEEAVSVQRSMQETVNKLHQKEEQFNMLSSDLEKLRENLADMEAKFREKDEREEQLIKAKEKLENDIAEIMKMSGDNSSQLTKMNDELRLKERDVEELQLKLTKANENASFLQKSIEDMTVKAEQSQQEAAKKHEEEKKELERKLSDLEKKMETSHNQCQELKARYERATSETKTKHEEILQNLQKTLLDTEDKLKGAREENSGLLQELEELRKQADKAKAAQTAEDAMQIMEQMTKEKTETLASLEDTKQTNAKLQNELDTLKENNLKNVEELNKSKELLTVENQKMEEFRKEIETLKQAAAQKSQQLSALQEENVKLAEELGRSRDEVTSHQKLEEERSVLNNQLLEMKKRESKFIKDADEEKASLQKSISITSALLTEKDAELEKLRNEVTVLRGENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF dctn1_human Dynactin subunit 1 DCTN1 150 kDa dynein-associated polypeptide DAP-150 p150-glued p135 protein protein human Homo sapiens Human MAQSKRHVYSRTPSGSRMSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLRGLKPKKAPTARKTTTRRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEEQQRGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS clip1-dctn1-2 3160899 N/A 3160909 unspecified role unspecified role unspecified role unspecified role Region 1406-1427 Region 1406-1427 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160910 unspecified role unspecified role unspecified role unspecified role Region 16-107 Region 16-107 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false clip1-clip1-1 3160900 N/A 3160909 unspecified role unspecified role prey prey Region 56-128 Region 56-128 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P30622-1:p.Lys98Glu K98E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P30622-1:p.Arg120Glu R120E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160909 unspecified role unspecified role bait bait Region 1406-1427 Region 1406-1427 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli putative self interaction putative self interaction false false false clip1-clip1-2 3160901 N/A 3160909 unspecified role unspecified role prey prey Region 56-128 Region 56-128 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P30622-1:p.Lys98Glu K98E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P30622-1:p.Arg120Glu R120E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160909 unspecified role unspecified role bait bait Region 1364-1427 Region 1364-1427 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli putative self interaction putative self interaction false false false clip1-clip1-3 3160902 N/A 3160909 unspecified role unspecified role unspecified role unspecified role Region 56-128 Region 56-128 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160909 unspecified role unspecified role unspecified role unspecified role Region 1364-1427 Region 1364-1427 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli putative self interaction putative self interaction false false false clip1-clip1-4 3160903 N/A 3160909 unspecified role unspecified role prey prey Region 210-282 Region 210-282 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P30622-1:p.Lys252Glu K252E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P30622-1:p.His276Glu H276E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160909 unspecified role unspecified role bait bait Region 1406-1427 Region 1406-1427 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli putative self interaction putative self interaction false false false clip1-clip1-5 3160904 N/A 3160909 unspecified role unspecified role prey prey Region 210-282 Region 210-282 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P30622-1:p.Lys252Glu K252E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P30622-1:p.His276Glu H276E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P30622-1:p.Lys238Glu K238E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160909 unspecified role unspecified role bait bait Region 1364-1427 Region 1364-1427 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli putative self interaction putative self interaction false false false clip1-dctn1-3 3160905 N/A 3160909 unspecified role unspecified role bait bait Region 1406-1427 Region 1406-1427 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160910 unspecified role unspecified role prey prey Region 16-107 Region 16-107 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q14203:p.Lys68Ala K68A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false clip1-clip1-6 3160906 N/A 3160909 unspecified role unspecified role prey prey Region 210-282 Region 210-282 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P30622-1:p.Lys238Glu K238E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160909 unspecified role unspecified role bait bait Region 1364-1390 Region 1364-1390 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli putative self interaction putative self interaction false false false clip1-clip1-7 3160907 N/A 3160909 unspecified role unspecified role unspecified role unspecified role Region 210-282 Region 210-282 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160909 unspecified role unspecified role unspecified role unspecified role Region 1364-1427 Region 1364-1427 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli putative self interaction putative self interaction false false false clip1-clip1-8 3160908 N/A 3160909 unspecified role unspecified role prey prey Region 56-128 Region 56-128 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160909 unspecified role unspecified role bait bait Region 1364-1390 Region 1364-1390 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli putative self interaction putative self interaction false false false