DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ binnerts-2007-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Binnerts ME., Kim KA., Bright JM., Patel SM., Tran K., Zhou M., Leung JM., Liu Y., Lomas WE., Dixon M., Hazell SA., Wagle M., Nie WS., Tomasevic N., Williams J., Zhan X., Levy MD., Funk WD., Abo A. DIP original experiment: DIP-113876X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6. Binnerts ME., Kim KA., Bright JM., Patel SM., Tran K., Zhou M., Leung JM., Liu Y., Lomas WE., Dixon M., Hazell SA., Wagle M., Nie WS., Tomasevic N., Williams J., Zhan X., Levy MD., Funk WD., Abo A. Only protein-protein interactions imex curation binnerts-2007-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Binnerts ME., Kim KA., Bright JM., Patel SM., Tran K., Zhou M., Leung JM., Liu Y., Lomas WE., Dixon M., Hazell SA., Wagle M., Nie WS., Tomasevic N., Williams J., Zhan X., Levy MD., Funk WD., Abo A. DIP original experiment: DIP-113877X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6. Binnerts ME., Kim KA., Bright JM., Patel SM., Tran K., Zhou M., Leung JM., Liu Y., Lomas WE., Dixon M., Hazell SA., Wagle M., Nie WS., Tomasevic N., Williams J., Zhan X., Levy MD., Funk WD., Abo A. Only protein-protein interactions imex curation binnerts-2007-3 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Binnerts ME., Kim KA., Bright JM., Patel SM., Tran K., Zhou M., Leung JM., Liu Y., Lomas WE., Dixon M., Hazell SA., Wagle M., Nie WS., Tomasevic N., Williams J., Zhan X., Levy MD., Funk WD., Abo A. DIP original experiment: DIP-113878X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6. Binnerts ME., Kim KA., Bright JM., Patel SM., Tran K., Zhou M., Leung JM., Liu Y., Lomas WE., Dixon M., Hazell SA., Wagle M., Nie WS., Tomasevic N., Williams J., Zhan X., Levy MD., Funk WD., Abo A. Only protein-protein interactions imex curation dkk1_human Dickkopf-related protein 1 SK DKK1 UNQ492/PRO1008 protein protein human Homo sapiens Human MMALGAAGATRVFVAMVAAALGGHPLLGVSATLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH lrp6_mouse Low-density lipoprotein receptor-related protein 6 Lrp6 protein protein mouse Mus musculus Mouse MGAVLRSLLACSFCVLLRAAPLLLYANRRDLRLVDATNGKENATIVVGGLEDAAAVDFVFGHGLIYWSDVSEEAIKRTEFNKSESVQNVVVSGLLSPDGLACDWLGEKLYWTDSETNRIEVSNLDGSLRKVLFWQELDQPRAIALDPSSGFMYWTDWGEVPKIERAGMDGSSRFVIINTEIYWPNGLTLDYQERKLYWADAKLNFIHKSNLDGTNRQAVVKGSLPHPFALTLFEDTLYWTDWNTHSILACNKYTGEGLREIHSNIFSPMDIHAFSQQRQPNATNPCGIDNGGCSHLCLMSPVKPFYQCACPTGVKLMENGKTCKDGATELLLLARRTDLRRISLDTPDFTDIVLQLEDIRHAIAIDYDPVEGYIYWTDDEVRAIRRSFIDGSGSQFVVTAQIAHPDGIAVDWVARNLYWTDTGTDRIEVTRLNGTMRKILISEDLEEPRAIVLDPMVGYMYWTDWGEIPKIERAALDGSDRVVLVNTSLGWPNGLALDYDEGTIYWGDAKTDKIEVMNTDGTGRRVLVEDKIPHIFGFTLLGDYVYWTDWQRRSIERVHKRSAEREVIIDQLPDLMGLKATSVHRVIGSNPCAEDNGGCSHLCLYRPQGLRCACPIGFELIGDMKTCIVPEAFLLFSRRADIRRISLETNNNNVAIPLTGVKEASALDFDVTDNRIYWTDISLKTISRAFMNGSALEHVVEFGLDYPEGMAVDWLGKNLYWADTGTNRIEVSKLDGQHRQVLVWKDLDSPRALALDPAEGFMYWTEWGGKPKIDRAAMDGSERTTLVPNVGRANGLTIDYAKRRLYWTDLDTNLIESSDMLGLNREVIADDLPHPFGLTQYQDYIYWTDWSRRSIERANKTSGQNRTIIQGHLDYVMDILVFHSSRQAGWNECASSNGHCSHLCLAVPVGGFVCGCPAHYSLNADNRTCSAPSTFLLFSQKSAINRMVIDEQQSPDIILPIHSLRNVRAIDYDPLDKQLYWIDSRQNSIRKAHEDGGQGFNVVANSVANQNLEIQPYDLSIDIYSRYIYWTCEATNVIDVTRLDGRSVGVVLKGEQDRPRAIVVNPEKGYMYFTNLQERSPKIERAALDGTEREVLFFSGLSKPIALALDSKLGKLFWADSDLRRIESSDLSGANRIVLEDSNILQPVGLTVFENWLYWIDKQQQMIEKIDMTGREGRTKVQARIAQLSDIHAVKELNLQEYRQHPCAQDNGGCSHICLVKGDGTTRCSCPMHLVLLQDELSCGEPPTCSPQQFTCFTGDIDCIPVAWRCDGFTECEDHSDELNCPVCSESQFQCASGQCIDGALRCNGDANCQDKSDEKNCEVLCLIDQFRCANGQCVGKHKKCDHSVDCSDRSDELDCYPTEEPAPQATNTVGSVIGVIVTIFVSGTIYFICQRMLCPRMKGDGETMTNDYVVHSPASVPLGYVPHPSSLSGSLPGMSRGKSMISSLSIMGGSSGPPYDRAHVTGASSSSSSSTKGTYFPAILNPPPSPATERSHYTMEFGYSSNSPSTHRSYSYRPYSYRHFAPPTTPCSTDVCDSDYAPSRRMTSVATAKGYTSDVNYDSEPVPPPPTPRSQYLSAEENYESCPPSPYTERSYSHHLYPPPPSPCTDSS krem1_human Kremen protein 1 KREMEN1 KREMEN KRM1 Dickkopf receptor Kringle domain-containing transmembrane protein 1 Kringle-containing protein marking the eye and the nose protein protein human Homo sapiens Human MAPPAARLALLSAAALTLAARPAPSPGLGPECFTANGADYRGTQNWTALQGGKPCLFWNETFQHPYNTLKYPNGEGGLGEHNYCRNPDGDVSPWCYVAEHEDGVYWKYCEIPACQMPGNLGCYKDHGNPPPLTGTSKTSNKLTIQTCISFCRSQRFKFAGMESGYACFCGNNPDYWKYGEAASTECNSVCFGDHTQPCGGDGRIILFDTLVGACGGNYSAMSSVVYSPDFPDTYATGRVCYWTIRVPGASHIHFSFPLFDIRDSADMVELLDGYTHRVLARFHGRSRPPLSFNVSLDFVILYFFSDRINQAQGFAVLYQAVKEELPQERPAVNQTVAEVITEQANLSVSAARSSKVLYVITTSPSHPPQTVPGSNSWAPPMGAGSHRVEGWTVYGLATLLILTVTAIVAKILLHVTFKSHRVPASGDLRDCHQPGTSGEIWSIFYKPSTSISIFKKKLKGQSQQDDRNPLVSD dkk1-lrp6-2 3160717 N/A 3160720 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3160721 unspecified role unspecified role unspecified role unspecified role mouse Mus musculus Mouse direct interaction direct interaction false false false kremen1-lrp6-1 3160718 N/A 3160722 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3160721 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false kremen1-dkk1-1 3160719 N/A 3160720 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3160722 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false