DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mitin-2007-1 in vitro In vitro gdp_gtp exchange gdp/gtp exchange assay gtp/gdp exchange guanine nucleotide exchange assay gtp/gdp exchange guanine nucleotide exchange assay gdp_gtp exchange predetermined predetermined participant predetermined Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. DIP original experiment: DIP-255319X Nature structural & molecular biology 2007 IMEx Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression. Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. Only protein-protein interactions imex curation mitin-2007-2 in vitro In vitro gdp_gtp exchange gdp/gtp exchange assay gtp/gdp exchange guanine nucleotide exchange assay gtp/gdp exchange guanine nucleotide exchange assay gdp_gtp exchange predetermined predetermined participant predetermined Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. DIP original experiment: DIP-255317X Nature structural & molecular biology 2007 IMEx Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression. Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. Only protein-protein interactions imex curation mitin-2007-3 in vitro In vitro gdp_gtp exchange gdp/gtp exchange assay gtp/gdp exchange guanine nucleotide exchange assay gtp/gdp exchange guanine nucleotide exchange assay gdp_gtp exchange predetermined predetermined participant predetermined Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. DIP original experiment: DIP-255321X Nature structural & molecular biology 2007 IMEx Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression. Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. Only protein-protein interactions imex curation mitin-2007-4 in vitro In vitro gdp_gtp exchange gdp/gtp exchange assay gtp/gdp exchange guanine nucleotide exchange assay gtp/gdp exchange guanine nucleotide exchange assay gdp_gtp exchange predetermined predetermined participant predetermined Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. DIP original experiment: DIP-255320X Nature structural & molecular biology 2007 IMEx Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression. Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. Only protein-protein interactions imex curation mitin-2007-5 in vitro In vitro gdp_gtp exchange gdp/gtp exchange assay gtp/gdp exchange guanine nucleotide exchange assay gtp/gdp exchange guanine nucleotide exchange assay gdp_gtp exchange predetermined predetermined participant predetermined Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. DIP original experiment: DIP-255318X Nature structural & molecular biology 2007 IMEx Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression. Mitin N., Betts L., Yohe ME., Der CJ., Sondek J., Rossman KL. Only protein-protein interactions imex curation mcf2l_mouse Guanine nucleotide exchange factor DBS DBL's big sister MCF2-transforming sequence-like protein Mcf2l Dbs protein protein mouse Mus musculus Mouse MSDCWCFIFCKEHVRSNPLSPQHDGASREEADHQVDVSDGIRLVPDKAEATAATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALKEGNSILESLREPLAESAAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRRRGLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRNIPAGLESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSSTSPTKGNTRNVKKLEDRKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVPGKYTVVMDDEKGGPDTLAMRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGKIHCARQLCPEPAEILSPEPV p60953-2 Cell division control protein 42 homolog CDC42 G25K GTP-binding protein Placental protein protein human Homo sapiens Human MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL q9nr80-1 Rho guanine nucleotide exchange factor 4 ARHGEF4 KIAA1112 APC-stimulated guanine nucleotide exchange factor 1 protein protein human Homo sapiens Human MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK rhoa_human Transforming protein RhoA Rho cDNA clone 12 ARHA RHO12 RHOA ARH12 protein protein human Homo sapiens Human MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL p63000-1 Ras-related C3 botulinum toxin substrate 1 RAC1 Cell migration-inducing gene 5 protein Ras-like protein TC25 TC25 p21-Rac1 MIG5 Rac1A protein protein human Homo sapiens Human MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL mint tiam1_mouse Rho guanine nucleotide exchange factor TIAM1 T-lymphoma invasion and metastasis-inducing protein 1 Tiam1 Tiam-1 protein protein mouse Mus musculus Mouse MGNAESQNVDHEFYGEKHASLGRKHTSRSLRLSHKTRRTRHASSGKAIHRNSEVSTRSSSTPSIPQSLAENGLEPFSQEGALDDFGDPIWVDRVDMGLRPVSYTDSSVTPSVDGSIVLTAASVQSMPDSEESRLYGDDATYLAEGGRRQCPYTSNGPTFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARGMETEASPRQLSTCQRANSLGDLYAQKNSGVKANGGPRNRFSSYCRNLVSDIPDLAKHKMPPAAAEETPPYSNYNTLPCRKSHCLSEGATNPQISLSKSMQGRRAKTTQDVNTGEGSEFADSGIEGATTDTDLLSRRSNATNSSYSPPTGRAFVGSDSGSSSTGDRARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYETDGRSGIDHNSVPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGEIGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGRPTINQVFGEGTDAVKRSLEGIFDDTVPDGKREKEVVLPSVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKTHQLDHSAHYLRLKFLMENRVQFYIPQPEEDIYELLYKEIEICPKVTQNIHIEKSDAAADNYGFLLSSVDEDGIRRLYVNSVKETGLASKKGLKAGDEILEINNRAAGTLNSSMLKDFLSQPSLGLLVRTYPEPEGGVELLENPPHRVDGPVDLGESPLAFLTSNPGHSLSSEQGSSAETAPEEGEGPDLESSDETDHSSKSTEQVAAFCRSLHEMSPSDSSPSPQDATSPQLATTRQLSDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDTAFKAFLDAQNPRQQHSSTLESYLIKPIQRVLKYPLLLRELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTSVIWLNPPASLGKWKKEPELAAFVFKTAVVLVYKDGSKQKKKLVGSHRLSIYEEWDPFRFRHMIPTEALQVRALPSADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKSVHSILRDKHRRQLLKTESLPSAQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAISASSPEKEPQQPAGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESLKGASVDRDLQEQLQAASISQRARGRRTLDSHASRMTQLKKQAALSGINGGLESASEEVIWVRREDFAPSRKLNTEI p60953-1 Cell division control protein 42 homolog CDC42 G25K GTP-binding protein Brain protein protein human Homo sapiens Human MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQRGLKNVFDEAILAALEPPETQPKRKCCIF cdc42-mcf2l-1 3160080 N/A 3160085 unspecified role unspecified role unspecified role unspecified role Region 623-967 Region 623-967 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160086 unspecified role unspecified role unspecified role unspecified role Region 1-188 Region 1-188 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false arhgef4-cdc42-2 3160081 N/A 3160087 unspecified role unspecified role unspecified role unspecified role Region 271-632 Region 271-632 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160086 unspecified role unspecified role unspecified role unspecified role Region 1-188 Region 1-188 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mcf2l-rhoa-1 3160082 N/A 3160088 unspecified role unspecified role unspecified role unspecified role Region 1-190 Region 1-190 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160085 unspecified role unspecified role unspecified role unspecified role Region 623-967 Region 623-967 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rac1-tiam1-3 3160083 N/A 3160089 unspecified role unspecified role unspecified role unspecified role Region 1-189 Region 1-189 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160090 unspecified role unspecified role unspecified role unspecified role Region 1033-1406 Region 1033-1406 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false arhgef4-cdc42-3 3160084 N/A 3160091 unspecified role unspecified role unspecified role unspecified role Region 1-188 Region 1-188 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3160087 unspecified role unspecified role unspecified role unspecified role Region 271-632 Region 271-632 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false