DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sirajuddin-2007-1 ecolx Escherichia coli 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. DIP original experiment: DIP-253087X Nature 2007 IMEx Structural insight into filament formation by mammalian septins. Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. Only protein-protein interactions imex curation sirajuddin-2007-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. DIP original experiment: DIP-253083X Nature 2007 IMEx Structural insight into filament formation by mammalian septins. Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. Only protein-protein interactions imex curation sirajuddin-2007-3 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. DIP original experiment: DIP-253089X Nature 2007 IMEx Structural insight into filament formation by mammalian septins. Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. Only protein-protein interactions imex curation sirajuddin-2007-4 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. DIP original experiment: DIP-253090X Nature 2007 IMEx Structural insight into filament formation by mammalian septins. Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. Only protein-protein interactions imex curation sirajuddin-2007-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. DIP original experiment: DIP-253086X Nature 2007 IMEx Structural insight into filament formation by mammalian septins. Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. Only protein-protein interactions imex curation sirajuddin-2007-6 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. DIP original experiment: DIP-253084X Nature 2007 IMEx Structural insight into filament formation by mammalian septins. Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. Only protein-protein interactions imex curation sirajuddin-2007-7 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. DIP original experiment: DIP-253088X Nature 2007 IMEx Structural insight into filament formation by mammalian septins. Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. Only protein-protein interactions imex curation sirajuddin-2007-8 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. DIP original experiment: DIP-253085X Nature 2007 IMEx Structural insight into filament formation by mammalian septins. Sirajuddin M., Farkasovsky M., Hauer F., Kühlmann D., Macara IG., Weyand M., Stark H., Wittinghofer A. Only protein-protein interactions imex curation sept7_human Septin-7 SEPTIN7 CDC10 CDC10 protein homolog SEPT7 protein protein human Homo sapiens Human MSVSARSAAAEERSVNSSTMVAQQKNLEGYVGFANLPNQVYRKSVKRGFEFTLMVVGESGLGKSTLINSLFLTDLYSPEYPGPSHRIKKTVQVEQSKVLIKEGGVQLLLTIVDTPGFGDAVDNSNCWQPVIDYIDSKFEDYLNAESRVNRRQMPDNRVQCCLYFIAPSGHGLKPLDIEFMKRLHEKVNIIPLIAKADTLTPEECQQFKKQIMKEIQEHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENGEHCDFTILRNMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNKNKGQLTKSPLAQMEEERREHVAKMKKMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRQFEDEKANWEAQQRILEQQNSSRTLEKNKKKGKIF sept6_human Septin-6 SEPTIN6 KIAA0128 SEP2 SEPT6 protein protein human Homo sapiens Human MAATDIARQVGEGCRTVPLAGHVGFDSLPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPATHTQPGVQLQSNTYDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE q15019-1 Septin-2 SEPTIN2 DIFF6 KIAA0158 NEDD5 Neural precursor cell expressed developmentally down-regulated protein 5 SEPT2 protein protein human Homo sapiens Human MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV cdc12_yeast Cell division control protein 12 CDC12 CLA10 PSL7 YHR107C Septin protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAATATAAPVPPPVGISNLPNQRYKIVNEEGGTFTVMLCGESGLGKTTFINTLFQTVLKRADGQQHRQEPIRKTVEIDITRALLEEKHFELRVNVIDTPGFGDNVNNNKAWQPLVDFIDDQHDSYMRQEQQPYRTKKFDLRVHAVLYFIRPTGHGLKPIDIETMKRLSTRANLIPVIAKADTLTAQELQQFKSRIRQVIEAQEIRIFTPPLDADSKEDAKSGSNPDSAAVEHARQLIEAMPFAIVGSEKKFDNGQGTQVVARKYPWGLVEIENDSHCDFRKLRALLLRTYLLDLISTTQEMHYETYRRLRLEGHENTGEGNEDFTLPAIAPARKLSHNPRYKEEENALKKYFTDQVKAEEQRFRQWEQNIVNERIRLNGDLEEIQGKVKKLEEQVKSLQVKKSHLK A72DC393A6DBF924 cdc11_yeast Cell division control protein 11 CDC11 PSL9 YJR076C J1833 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGIIDASSALRKRKHLKRGITFTVMIVGQSGSGRSTFINTLCGQQVVDTSTTILLPTDTSTEIDLQLREETVELEDDEGVKIQLNIIDTPGFGDSLDNSPSFEIISDYIRHQYDEILLEESRVRRNPRFKDGRVHCCLYLINPTGHGLKEIDVEFIRQLGSLVNIIPVISKSDSLTRDELKLNKKLIMEDIDRWNLPIYNFPFDEDEISDEDYETNMYLRTLLPFAIIGSNEVYEMGGDVGTIRGRKYPWGILDVEDSSISDFVILRNALLISHLHDLKNYTHEILYERYRTEALSGESVAAESIRPNLTKLNGSSSSSTTTRRNTNPFKQSNNINNDVLNPASDMHGQSTGENNETYMTREEQIRLEEERLKAFEERVQQELLLKRQELLQREKELREIEARLEKEAKIKQEE 5CFFD4CD1F6C57D4 cdc10_yeast Cell division control protein 10 CDC10 YCR2C YCR002C YCR022 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDPLSSVQPASYVGFDTITNQIEHRLLKKGFQFNIMVVGQSGLGKSTLINTLFASHLIDSATGDDISALPVTKTTEMKISTHTLVEDRVRLNINVIDTPGFGDFIDNSKAWEPIVKYIKEQHSQYLRKELTAQRERFITDTRVHAILYFLQPNGKELSRLDVEALKRLTEIANVIPVIGKSDTLTLDERTEFRELIQNEFEKYNFKIYPYDSEELTDEELELNRSVRSIIPFAVVGSENEIEINGETFRGRKTRWSAINVEDINQCDFVYLREFLIRTHLQDLIETTSYIHYEGFRARQLIALKENANSRSSAHMSSNAIQR 672467301D4D2697 cdc3_yeast Cell division control protein 3 CDC3 L8543.7 YLR314C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLKEEQVSIKQDPEQEERQHDQFNDVQIKQESQDHDGVDSQYTNGTQNDDSERFEAAESDVKVEPGLGMGITSSQSEKGQVLPDQPEIKFIRRQINGYVGFANLPKQWHRRSIKNGFSFNLLCVGPDGIGKTTLMKTLFNNDDIEANLVKDYEEELANDQEEEEGQGEGHENQSQEQRHKVKIKSYESVIEENGVKLNLNVIDTEGFGDFLNNDQKSWDPIIKEIDSRFDQYLDAENKINRHSINDKRIHACLYFIEPTGHYLKPLDLKFMQSVYEKCNLIPVIAKSDILTDEEILSFKKTIMNQLIQSNIELFKPPIYSNDDAENSHLSERLFSSLPYAVIGSNDIVENYSGNQVRGRSYPWGVIEVDNDNHSDFNLLKNLLIKQFMEELKERTSKILYENYRSSKLAKLGIKQDNSVFKEFDPISKQQEEKTLHEAKLAKLEIEMKTVFQQKVSEKEKKLQKSETELFARHKEMKEKLTKQLKALEDKKKQLELSINSASPNVNHSPVPTKKKGFLR DCCD4C8C16AA5E25 sept2-sept6-4 3158999 N/A 3159007 unspecified role unspecified role unspecified role unspecified role Region 1-305 Region 1-305 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3159008 unspecified role unspecified role unspecified role unspecified role Region 1-310 Region 1-310 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3159009 unspecified role unspecified role unspecified role unspecified role Region 1-308 Region 1-308 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false sept2-1 3159000 N/A 3159009 unspecified role unspecified role unspecified role unspecified role Region 1-315 Region 1-315 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q15019-1:p.Phe156Asp F156D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q15019-1:p.Trp260Ala W260A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q15019-1:p.His270Asp H270D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sept2-sept6-5 3159001 N/A 3159007 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-305 Region 1-305 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3159008 unspecified role unspecified role prey prey Region 1-310 Region 1-310 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3159009 unspecified role unspecified role bait bait MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-308 Region 1-308 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false sept7-sept2-1 3159002 N/A 3159007 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-305 Region 1-305 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3159008 unspecified role unspecified role prey prey Region 1-310 Region 1-310 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3159009 unspecified role unspecified role prey prey MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-308 Region 1-308 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false sept2-sept6-6 3159003 N/A 3159007 unspecified role unspecified role unspecified role unspecified role Region 1-305 Region 1-305 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3159008 unspecified role unspecified role unspecified role unspecified role Region 1-310 Region 1-310 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3159009 unspecified role unspecified role unspecified role unspecified role Region 1-308 Region 1-308 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false sept2-2 3159004 N/A 3159009 unspecified role unspecified role unspecified role unspecified role Region 1-315 Region 1-315 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sept2-sept6-7 3159005 N/A 3159007 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3159008 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3159009 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cdc10-cdc11-5 3159006 N/A 3159010 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3159011 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3159012 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3159013 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false