DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ taddeo-2007-1 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Taddeo B., Sciortino MT., Zhang W., Roizman B. DIP original experiment: DIP-261880X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Interaction of herpes simplex virus RNase with VP16 and VP22 is required for the accumulation of the protein but not for accumulation of mRNA. Taddeo B., Sciortino MT., Zhang W., Roizman B. Only protein-protein interactions imex curation taddeo-2007-2 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Taddeo B., Sciortino MT., Zhang W., Roizman B. DIP original experiment: DIP-261878X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Interaction of herpes simplex virus RNase with VP16 and VP22 is required for the accumulation of the protein but not for accumulation of mRNA. Taddeo B., Sciortino MT., Zhang W., Roizman B. Only protein-protein interactions imex curation taddeo-2007-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Taddeo B., Sciortino MT., Zhang W., Roizman B. DIP original experiment: DIP-261881X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Interaction of herpes simplex virus RNase with VP16 and VP22 is required for the accumulation of the protein but not for accumulation of mRNA. Taddeo B., Sciortino MT., Zhang W., Roizman B. Only protein-protein interactions imex curation taddeo-2007-4 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Taddeo B., Sciortino MT., Zhang W., Roizman B. DIP original experiment: DIP-261879X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Interaction of herpes simplex virus RNase with VP16 and VP22 is required for the accumulation of the protein but not for accumulation of mRNA. Taddeo B., Sciortino MT., Zhang W., Roizman B. Only protein-protein interactions imex curation vp22_hhv11 Tegument protein VP22 UL49 protein protein hhv11 Human herpesvirus 1 (strain 17) (HHV-1) Human herpes simplex virus 1 HHV-1 MTSRRSVKSGPREVPRDEYEDLYYTPSSGMASPDSPPDTSRRGALQTRSRQRGEVRFVQYDESDYALYGGSSSEDDEHPEVPRTRRPVSGAVLSGPGPARAPPPPAGSGGAGRTPTTAPRAPRTQRVATKAPAAPAAETTRGRKSAQPESAALPDAPASTAPTRSKTPAQGLARKLHFSTAPPNPDAPWTPRVAGFNKRVFCAAVGRLAAMHARMAAVQLWDMSRPRTDEDLNELLGITTIRVTVCEGKNLLQRANELVNPDVVQDVDAATATRGRSAASRPTERPRAPARSASRPRRPVE mint vp16_hhv11 Tegument protein VP16 Vmw65 ICP25 Alpha-TIF Alpha trans-inducing protein UL48 protein protein hhv11 Human herpesvirus 1 (strain 17) (HHV-1) Human herpes simplex virus 1 HHV-1 MDLLVDELFADMNADGASPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAALFNRLLDDLGFSAGPALCTMLDTWNEDLFSALPTNADLYRECKFLSTLPSDVVEWGDAYVPERTQIDIRAHGDVAFPTLPATRDGLGLYYEALSRFFHAELRAREESYRTVLANFCSALYRYLRASVRQLHRQAHMRGRDRDLGEMLRATIADRYYRETARLARVLFLHLYLFLTREILWAAYAEQMMRPDLFDCLCCDLESWRQLAGLFQPFMFVNGALTVRGVPIEARRLRELNHIREHLNLPLVRSAATEEPGAPLTTPPTLHGNQARASGYFMVLIRAKLDSYSSFTTSPSEAVMREHAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG mint Responsible for transcriptional activation of immediate early promoters (alpha genes). Activation requires the formation of a heteromeric complex with the host cell factor. These two proteins then assemble with the octamer motif-binding protein Oct-1 on the cis-acting target sequence: 5'-TAATGARAT-3' shut_hhv11 Virion host shutoff protein UL41 protein protein hhv11 Human herpesvirus 1 (strain 17) (HHV-1) Human herpes simplex virus 1 HHV-1 MGLFGMMKFAHTHHLVKRRGLGAPAGYFTPIAVDLWNVMYTLVVKYQRRYPSYDREAITLHCLCRLLKVFTQKSLFPIFVTDRGVNCMEPVVFGAKAILARTTAQCRTDEEASDVDASPPPSPITDSRPSSAFSNMRRRGTSLASGTRGTAGSGAALPSAAPSKPALRLAHLFCIRVLRALGYAYINSGQLEADDACANLYHTNTVAYVYTTDTDLLLMGCDIVLDISACYIPTINCRDILKYFKMSYPQFLALFVRCHTDLHPNNTYASVEDVLRECHWTPPSRSQTRRAIRREHTSSRSTETRPPLPPAAGGTETRVSWTEILTQQIAGGYEDDEDLPLDPRDVTGGHPGPRSSSSEILTPPELVQVPNAQLLEEHRSYVANPRRHVIHDAPESLDWLPDPMTITELVEHRYIKYVISLIGPKERGPWTLLKRLPIYQDIRDENLARSIVTRHITAPDIADRFLEQLRTQAPPPAFYKDVLAKFWDE vp16_hhv11-vp22_hhv11-1 3158656 N/A 3158660 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158661 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false ul41-vp16_hhv11-1 3158657 N/A 3158660 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158661 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158662 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false ul41-vp16_hhv11-2 3158658 N/A 3158660 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158661 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158662 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false ul41-vp16_hhv11-3 3158659 N/A 3158661 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3158662 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false