DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ scherr-2007-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. DIP original experiment: DIP-261769X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. Only protein-protein interactions imex curation scherr-2007-2 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. DIP original experiment: DIP-261770X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. Only protein-protein interactions imex curation scherr-2007-3 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. DIP original experiment: DIP-261771X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. Only protein-protein interactions imex curation pkng_myctu Serine/threonine-protein kinase PknG MTCY22G10.06c Rv0410c pknG protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MAKASETERSGPGTQPADAQTATSATVRPLSTQAVFRPDFGDEDNFPHPTLGPDTEPQDRMATTSRVRPPVRRLGGGLVEIPRAPDIDPLEALMTNPVVPESKRFCWNCGRPVGRSDSETKGASEGWCPYCGSPYSFLPQLNPGDIVAGQYEVKGCIAHGGLGWIYLALDRNVNGRPVVLKGLVHSGDAEAQAMAMAERQFLAEVVHPSIVQIFNFVEHTDRHGDPVGYIVMEYVGGQSLKRSKGQKLPVAEAIAYLLEILPALSYLHSIGLVYNDLKPENIMLTEEQLKLIDLGAVSRINSFGYLYGTPGFQAPEIVRTGPTVATDIYTVGRTLAALTLDLPTRNGRYVDGLPEDDPVLKTYDSYGRLLRRAIDPDPRQRFTTAEEMSAQLTGVLREVVAQDTGVPRPGLSTIFSPSRSTFGVDLLVAHTDVYLDGQVHAEKLTANEIVTALSVPLVDPTDVAASVLQATVLSQPVQTLDSLRAARHGALDADGVDFSESVELPLMEVRALLDLGDVAKATRKLDDLAERVGWRWRLVWYRAVAELLTGDYDSATKHFTEVLDTFPGELAPKLALAATAELAGNTDEHKFYQTVWSTNDGVISAAFGLARARSAEGDRVGAVRTLDEVPPTSRHFTTARLTSAVTLLSGRSTSEVTEEQIRDAARRVEALPPTEPRVLQIRALVLGGALDWLKDNKASTNHILGFPFTSHGLRLGVEASLRSLARVAPTQRHRYTLVDMANKVRPTSTF pkng-1 3158611 N/A 3158614 unspecified role unspecified role unspecified role unspecified role Region 74-750 Region 74-750 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pkng-pkng-1 3158612 N/A 3158614 enzyme target enzyme target substrate substrate unspecified role unspecified role P9WI73:p.Lys181Met K181M mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3158614 enzyme enzyme unspecified role unspecified role Region 74-750 Region 74-750 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false pkng-2 3158613 N/A 3158614 putative self putative self unspecified role unspecified role P9WI73:p.[Cys106Ser;Cys109Ser;Cys128Ser;Cys131Ser] C106/109/128/131S mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false